##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I AocI ApaLI AscI AseI
AsnI Asp718I AspEI AvrII BamHI
BciVI BglII BlnI BsaBI BsaWI
BseAI BsiEI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssSI Bst1107I
BstZ17I Bsu36I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoRI EcoT22I FseI HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NdeI NotI NsiI PacI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI ScaI
SgrAI SnaBI SpeI SspBI SspI
SwaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 5522 GACGTC GACGT^C
AccI 189 GTCTAC GT^[AC][GT]AC
AflII 1924 CTTAAG C^TTAAG
AflIII 193 ACACGT A^C[GA][CT]GT
Aor51HI 3034 AGCGCT AGC^GCT
AspI 6918 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 194 CACGTG CAC^GTG
BclI 1539 TGATCA T^GATCA
BfrI 1924 CTTAAG C^TTAAG
BsiWI 4585 CGTACG C^GTACG
BsmI 374 GAATGC GAATGC(1/-1)
BssHII 26 GCGCGC G^CGCGC
BstBI 1508 TTCGAA TT^CGAA
BstEII 2275 GGTCACC G^GT[ACGT]ACC
BstPI 2275 GGTCACC G^GT[ACGT]ACC
DraI 5257 TTTAAA TTT^AAA
DraIII 1790 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3034 AGCGCT AGC^GCT
EcoO65I 2275 GGTCACC G^GT[ACGT]ACC
EcoRV 758 GATATC GAT^ATC
FbaI 1539 TGATCA T^GATCA
HincII 1624 GTTGAC GT[CT]^[GA]AC
HindII 1624 GTTGAC GT[CT]^[GA]AC
NruI 8 TCGCGA TCG^CGA
NspV 1508 TTCGAA TT^CGAA
PflFI 6918 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 194 CACGTG CAC^GTG
PmlI 194 CACGTG CAC^GTG
SbfI 1246 CCTGCAGG CCTGCA^GG
SfuI 1508 TTCGAA TT^CGAA
Sse8387I 1246 CCTGCAGG CCTGCA^GG
Tth111I 6918 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 6831 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AviII 2907 TGCGCA TGC^GCA
5178 TGCGCA
BbvCI 1570 CCTCAGC CCTCAGC(-5/-2)
4613 GCTGAGG
BsaAI 194 CACGTG [CT]AC^GT[GA]
4587 TACGTG
BsmBI 787 GAGACG CGTCTC(1/5)
5520 GAGACG
FspI 2907 TGCGCA TGC^GCA
5178 TGCGCA
HgaI 3009 GACGC GACGC(5/10)
5388 GCGTC
HindIII 2828 AAGCTT A^AGCTT
3341 AAGCTT
NspI 4452 GCATGC [GA]CATG^[CT]
6045 GCATGC
PshAI 2482 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5534 GACTCGGGTC
SphI 4452 GCATGC GCATG^C
6045 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvI
BcgI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NheI NlaIII NlaIV PaeR7I PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SfiI SfoI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoI
XhoII XmaCI XmaI XmnI