##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AviII BclI BfrI BsaBI
BsaWI BseAI BsiEI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BsrFI
BssHII BstBI Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV FbaI FseI
FspI HpaI KpnI MamI MfeI
MluI MroI MunI NaeI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1623 GTATAC GT^[AC][GT]AC
Alw44I 3583 GTGCAC G^TGCAC
Aor51HI 4237 AGCGCT AGC^GCT
ApaLI 3583 GTGCAC G^TGCAC
BcgI 990 CGATTCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4500 GGATAC GTATCC(6/5)
BglII 3736 AGATCT A^GATCT
Bsp1407I 560 TGTACA T^GTACA
BsrGI 560 TGTACA T^GTACA
Bst1107I 1623 GTATAC GTA^TAC
BstZ17I 1623 GTATAC GTA^TAC
Eco47III 4237 AGCGCT AGC^GCT
EcoT22I 4799 ATGCAT ATGCA^T
HindIII 3793 AAGCTT A^AGCTT
KspI 15 CCGCGG CCGC^GG
NdeI 2194 CATATG CA^TATG
NheI 453 GCTAGC G^CTAGC
NsiI 4799 ATGCAT ATGCA^T
SacII 15 CCGCGG CCGC^GG
SmaI 978 CCCGGG CCC^GGG
SphI 4471 GCATGC GCATG^C
SspBI 560 TGTACA T^GTACA
XmaCI 978 CCCGGG C^CCGGG
XmaI 978 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AatII 4701 GACGTC GACGT^C
4807 GACGTC
AflIII 584 ACGTGT A^C[GA][CT]GT
5245 ACGTGT
AvrII 3374 CCTAGG C^CTAGG
3501 CCTAGG
BbeI 4747 GGCGCC GGCGC^C
5569 GGCGCC
BbrPI 2103 CACGTG CAC^GTG
3586 CACGTG
BlnI 3374 CCTAGG C^CTAGG
3501 CCTAGG
BsaAI 2103 CACGTG [CT]AC^GT[GA]
3586 CACGTG
BsmBI 905 GAGACG CGTCTC(1/5)
1072 GAGACG
BsrBI 2035 GAGCGG CCGCTC(-3/-3)
4024 GAGCGG
BssSI 3892 CACGAG CACGAG(-5/-1)
5479 CACGAG
HgaI 69 GACGC GACGC(5/10)
3380 GCGTC
HincII 1283 GTCAAC GT[CT]^[GA]AC
4294 GTCAAC
HindII 1283 GTCAAC GT[CT]^[GA]AC
4294 GTCAAC
KasI 4747 GGCGCC G^GCGCC
5569 GGCGCC
NarI 4747 GGCGCC GG^CGCC
5569 GGCGCC
NspI 479 ACATGC [GA]CATG^[CT]
4471 GCATGC
PmaCI 2103 CACGTG CAC^GTG
3586 CACGTG
PmlI 2103 CACGTG CAC^GTG
3586 CACGTG
SfoI 4747 GGCGCC GGC^GCC
5569 GGCGCC
SmlI 421 CTTGAG C^T[CT][GA]AG
4614 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI Asp700I AspHI AspI AvaI
AvaII BalI BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmnI