##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI Aor51HI AscI
Asp718I AspEI AviII AvrII BanI
BbeI BbrPI BcgI BlnI BsaHI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspEI BspLU11I BsrBI BsrGI BstEII
BstPI CpoI DraIII DrdI Eam1105I
Eco47III EcoNI EcoO65I FseI FspI
HaeII HgaI Hin1I KasI KpnI
MluI MroI NarI NcoI NotI
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI Sse8387I SspBI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflII 3758 CTTAAG C^TTAAG
Alw44I 4216 GTGCAC G^TGCAC
AocI 1899 CCTGAGG CC^T[ACGT]AGG
ApaLI 4216 GTGCAC G^TGCAC
AspI 458 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 743 GGATCC G^GATCC
BbsI 2810 GTCTTC GAAGAC(2/6)
BbvCI 5333 CCTCAGC CCTCAGC(-5/-2)
BciVI 2254 GGATAC GTATCC(6/5)
BfrI 3758 CTTAAG C^TTAAG
BlpI 334 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 334 GCTGAGC GC^T[ACGT]AGC
BpuAI 2810 GTCTTC GAAGAC(2/6)
BsaAI 4219 CACGTA [CT]AC^GT[GA]
BsmI 3999 GCATTC GAATGC(1/-1)
BspDI 1173 ATCGAT AT^CGAT
BssHII 93 GCGCGC G^CGCGC
BssSI 3187 CTCGTG CACGAG(-5/-1)
BstBI 5589 TTCGAA TT^CGAA
Bsu36I 1899 CCTGAGG CC^T[ACGT]AGG
CelII 334 GCTGAGC GC^T[ACGT]AGC
ClaI 1173 ATCGAT AT^CGAT
Eco81I 1899 CCTGAGG CC^T[ACGT]AGG
EcoRI 4154 GAATTC G^AATTC
EcoT14I 5132 CCAAGG C^C[AT][AT]GG
MfeI 5875 CAATTG C^AATTG
MunI 5875 CAATTG C^AATTG
NheI 215 GCTAGC G^CTAGC
NruI 145 TCGCGA TCG^CGA
NspV 5589 TTCGAA TT^CGAA
PflFI 458 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 713 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 5650 GACGGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1963 GAGCTC GAGCT^C
SapI 4742 GCTCTTC GCTCTTC(1/4)
ScaI 2617 AGTACT AGT^ACT
SfuI 5589 TTCGAA TT^CGAA
StuI 893 AGGCCT AGG^CCT
StyI 5132 CCAAGG C^C[AT][AT]GG
SwaI 3770 ATTTAAAT ATTT^AAAT
Tth111I 458 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 713 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 3639 GTATAC GT^[AC][GT]AC
3703 GTATAC
AlwNI 239 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
672 CAGATTCTG
Asp700I 1048 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5843 GAACAACTTC
AvaI 167 CTCGGG C^[CT]CG[GA]G
273 CCCGAG
BalI 1498 TGGCCA TGG^CCA
5766 TGGCCA
BclI 1877 TGATCA T^GATCA
2571 TGATCA
BglI 1 GCCTCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
102 GCCTCGCAGGC
BsgI 26 CTGCAC GTGCAG(16/14)
5112 CTGCAC
BsiEI 40 CGGCCG CG[GA][CT]^CG
298 CGGCCG
BsoBI 167 CTCGGG C^[CT]CG[GA]G
273 CCCGAG
BspHI 2682 TCATGA T^CATGA
3429 TCATGA
BspMI 796 ACCTGC ACCTGC(4/8)
1924 ACCTGC
Bst1107I 3639 GTATAC GTA^TAC
3703 GTATAC
BstAPI 238 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1790 GCACAGTATGC
BstZ17I 3639 GTATAC GTA^TAC
3703 GTATAC
DsaI 5 CCGCGG C^C[GA][CT]GG
37 CCGCGG
EagI 40 CGGCCG C^GGCCG
298 CGGCCG
EclXI 40 CGGCCG C^GGCCG
298 CGGCCG
Eco52I 40 CGGCCG C^GGCCG
298 CGGCCG
EcoRV 1556 GATATC GAT^ATC
2490 GATATC
EcoT22I 1316 ATGCAT ATGCA^T
1320 ATGCAT
FbaI 1877 TGATCA T^GATCA
2571 TGATCA
HpaI 1257 GTTAAC GTT^AAC
5883 GTTAAC
KspI 5 CCGCGG CCGC^GG
37 CCGCGG
MluNI 1498 TGGCCA TGG^CCA
5766 TGGCCA
MscI 1498 TGGCCA TGG^CCA
5766 TGGCCA
NsiI 1316 ATGCAT ATGCA^T
1320 ATGCAT
PstI 780 CTGCAG CTGCA^G
5235 CTGCAG
RcaI 2682 TCATGA T^CATGA
3429 TCATGA
SacII 5 CCGCGG CCGC^GG
37 CCGCGG
SmlI 3758 CTTAAG C^T[CT][GA]AG
5810 CTTGAG
SpeI 2824 ACTAGT A^CTAGT
5321 ACTAGT
XbaI 2333 TCTAGA T^CTAGA
5153 TCTAGA
XmnI 1048 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5843 GAACAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApaI ApoI AseI AsnI
AspHI AvaII BanII BbvI BcnI
BfaI BglII BmyI BpmI BsaBI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PshBI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SphI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII