################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflIII AgeI AhdI Aor51HI AscI Asp718I AspEI AviII AvrII BanI BbeI BbrPI BcgI BlnI BsaHI BsaWI BseAI BsiWI BsmBI Bsp1407I BspEI BspLU11I BsrBI BsrGI BstEII BstPI CpoI DraIII DrdI Eam1105I Eco47III EcoNI EcoO65I FseI FspI HaeII HgaI Hin1I KasI KpnI MluI MroI NarI NcoI NotI PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SspBI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AflII 3758 CTTAAG C^TTAAG Alw44I 4216 GTGCAC G^TGCAC AocI 1899 CCTGAGG CC^T[ACGT]AGG ApaLI 4216 GTGCAC G^TGCAC AspI 458 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 743 GGATCC G^GATCC BbsI 2810 GTCTTC GAAGAC(2/6) BbvCI 5333 CCTCAGC CCTCAGC(-5/-2) BciVI 2254 GGATAC GTATCC(6/5) BfrI 3758 CTTAAG C^TTAAG BlpI 334 GCTGAGC GC^T[ACGT]AGC Bpu1102I 334 GCTGAGC GC^T[ACGT]AGC BpuAI 2810 GTCTTC GAAGAC(2/6) BsaAI 4219 CACGTA [CT]AC^GT[GA] BsmI 3999 GCATTC GAATGC(1/-1) BspDI 1173 ATCGAT AT^CGAT BssHII 93 GCGCGC G^CGCGC BssSI 3187 CTCGTG CACGAG(-5/-1) BstBI 5589 TTCGAA TT^CGAA Bsu36I 1899 CCTGAGG CC^T[ACGT]AGG CelII 334 GCTGAGC GC^T[ACGT]AGC ClaI 1173 ATCGAT AT^CGAT Eco81I 1899 CCTGAGG CC^T[ACGT]AGG EcoRI 4154 GAATTC G^AATTC EcoT14I 5132 CCAAGG C^C[AT][AT]GG MfeI 5875 CAATTG C^AATTG MunI 5875 CAATTG C^AATTG NheI 215 GCTAGC G^CTAGC NruI 145 TCGCGA TCG^CGA NspV 5589 TTCGAA TT^CGAA PflFI 458 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 713 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 5650 GACGGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1963 GAGCTC GAGCT^C SapI 4742 GCTCTTC GCTCTTC(1/4) ScaI 2617 AGTACT AGT^ACT SfuI 5589 TTCGAA TT^CGAA StuI 893 AGGCCT AGG^CCT StyI 5132 CCAAGG C^C[AT][AT]GG SwaI 3770 ATTTAAAT ATTT^AAAT Tth111I 458 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 713 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccI 3639 GTATAC GT^[AC][GT]AC 3703 GTATAC AlwNI 239 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG 672 CAGATTCTG Asp700I 1048 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5843 GAACAACTTC AvaI 167 CTCGGG C^[CT]CG[GA]G 273 CCCGAG BalI 1498 TGGCCA TGG^CCA 5766 TGGCCA BclI 1877 TGATCA T^GATCA 2571 TGATCA BglI 1 GCCTCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 102 GCCTCGCAGGC BsgI 26 CTGCAC GTGCAG(16/14) 5112 CTGCAC BsiEI 40 CGGCCG CG[GA][CT]^CG 298 CGGCCG BsoBI 167 CTCGGG C^[CT]CG[GA]G 273 CCCGAG BspHI 2682 TCATGA T^CATGA 3429 TCATGA BspMI 796 ACCTGC ACCTGC(4/8) 1924 ACCTGC Bst1107I 3639 GTATAC GTA^TAC 3703 GTATAC BstAPI 238 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1790 GCACAGTATGC BstZ17I 3639 GTATAC GTA^TAC 3703 GTATAC DsaI 5 CCGCGG C^C[GA][CT]GG 37 CCGCGG EagI 40 CGGCCG C^GGCCG 298 CGGCCG EclXI 40 CGGCCG C^GGCCG 298 CGGCCG Eco52I 40 CGGCCG C^GGCCG 298 CGGCCG EcoRV 1556 GATATC GAT^ATC 2490 GATATC EcoT22I 1316 ATGCAT ATGCA^T 1320 ATGCAT FbaI 1877 TGATCA T^GATCA 2571 TGATCA HpaI 1257 GTTAAC GTT^AAC 5883 GTTAAC KspI 5 CCGCGG CCGC^GG 37 CCGCGG MluNI 1498 TGGCCA TGG^CCA 5766 TGGCCA MscI 1498 TGGCCA TGG^CCA 5766 TGGCCA NsiI 1316 ATGCAT ATGCA^T 1320 ATGCAT PstI 780 CTGCAG CTGCA^G 5235 CTGCAG RcaI 2682 TCATGA T^CATGA 3429 TCATGA SacII 5 CCGCGG CCGC^GG 37 CCGCGG SmlI 3758 CTTAAG C^T[CT][GA]AG 5810 CTTGAG SpeI 2824 ACTAGT A^CTAGT 5321 ACTAGT XbaI 2333 TCTAGA T^CTAGA 5153 TCTAGA XmnI 1048 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5843 GAACAACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApaI ApoI AseI AsnI AspHI AvaII BanII BbvI BcnI BfaI BglII BmyI BpmI BsaBI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI PshBI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SphI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII