##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
AhdI AscI AseI AsnI Asp700I
Asp718I AspEI AviII BamHI BbrPI
BciVI BsiWI BsmI BspDI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
Eam1105I EcoRI EcoT22I FseI FspI
HindIII HpaI KpnI MfeI MluI
MunI NdeI NheI NotI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SalI SfuI SgrAI SnaBI SpeI
SphI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 18 TCCGGA T^CCGGA
AflII 865 CTTAAG C^TTAAG
Alw44I 3991 GTGCAC G^TGCAC
ApaLI 3991 GTGCAC G^TGCAC
AvrII 4443 CCTAGG C^CTAGG
BcgI 6520 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 865 CTTAAG C^TTAAG
BglII 946 AGATCT A^GATCT
BlnI 4443 CCTAGG C^CTAGG
BsaAI 6562 TACGTG [CT]AC^GT[GA]
BseAI 18 TCCGGA T^CCGGA
Bsp1407I 2539 TGTACA T^GTACA
BspEI 18 TCCGGA T^CCGGA
BspHI 4840 TCATGA T^CATGA
BsrGI 2539 TGTACA T^GTACA
DraI 2220 TTTAAA TTT^AAA
DraIII 5128 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 923 GACGGCCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 5582 GATATC GAT^ATC
HincII 1329 GTCAAC GT[CT]^[GA]AC
HindII 1329 GTCAAC GT[CT]^[GA]AC
MroI 18 TCCGGA T^CCGGA
NcoI 4329 CCATGG C^CATGG
PshAI 4327 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4840 TCATGA T^CATGA
SbfI 968 CCTGCAGG CCTGCA^GG
ScaI 5680 AGTACT AGT^ACT
SfiI 1543 GGCCTCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 968 CCTGCAGG CCTGCA^GG
SspBI 2539 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AocI 103 CCTTAGG CC^T[ACGT]AGG
6645 CCTCAGG
Aor51HI 26 AGCGCT AGC^GCT
861 AGCGCT
BalI 2830 TGGCCA TGG^CCA
5332 TGGCCA
BbeI 1256 GGCGCC GGCGC^C
2768 GGCGCC
BlpI 3323 GCTGAGC GC^T[ACGT]AGC
5012 GCTGAGC
Bpu1102I 3323 GCTGAGC GC^T[ACGT]AGC
5012 GCTGAGC
BsaBI 1866 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6743 GATCCACATC
BsrBI 48 GAGCGG CCGCTC(-3/-3)
1107 GAGCGG
BssSI 4400 CACGAG CACGAG(-5/-1)
2251 CTCGTG
BstXI 2884 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4030 CCAGGGATCTGG
Bsu36I 103 CCTTAGG CC^T[ACGT]AGG
6645 CCTCAGG
CelII 3323 GCTGAGC GC^T[ACGT]AGC
5012 GCTGAGC
Eco47III 26 AGCGCT AGC^GCT
861 AGCGCT
Eco81I 103 CCTTAGG CC^T[ACGT]AGG
6645 CCTCAGG
KasI 1256 GGCGCC G^GCGCC
2768 GGCGCC
KspI 43 CCGCGG CCGC^GG
56 CCGCGG
MamI 1866 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6743 GATCCACATC
MluNI 2830 TGGCCA TGG^CCA
5332 TGGCCA
MscI 2830 TGGCCA TGG^CCA
5332 TGGCCA
NarI 1256 GGCGCC GG^CGCC
2768 GGCGCC
NruI 2664 TCGCGA TCG^CGA
3764 TCGCGA
PaeR7I 5341 CTCGAG C^TCGAG
5512 CTCGAG
SacII 43 CCGCGG CCGC^GG
56 CCGCGG
SexAI 4316 ACCAGGT A^CC[AT]GGT
5662 ACCTGGT
SfoI 1256 GGCGCC GGC^GCC
2768 GGCGCC
XbaI 4574 TCTAGA T^CTAGA
5729 TCTAGA
XhoI 5341 CTCGAG C^TCGAG
5512 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AspI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BclI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspLU11I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI