################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## Acc65I AccIII AclI AflIII Alw44I Aor51HI ApaLI AscI Asp700I Asp718I AviII BbrPI BclI BsaBI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrGI BssSI Bst1107I BstBI BstXI BstZ17I ClaI CpoI EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HpaI KpnI MamI MluI MroI NdeI NheI NotI NruI NsiI NspV PacI PmaCI PmeI PmlI Psp1406I PvuI RsrII SbfI SexAI SfiI SfuI SgrAI SpeI Sse8387I SspBI SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 3537 GACGTC GACGT^C AhdI 4964 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1638 CCTTAGG CC^T[ACGT]AGG ApaI 1437 GGGCCC GGGCC^C AspEI 4964 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2213 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1209 CCTAGG C^CTAGG BalI 10 TGGCCA TGG^CCA BbeI 149 GGCGCC GGCGC^C BciVI 976 GGATAC GTATCC(6/5) BlnI 1209 CCTAGG C^CTAGG BsiEI 122 CGACCG CG[GA][CT]^CG BspHI 2928 TCATGA T^CATGA BssHII 174 GCGCGC G^CGCGC BstEII 3286 GGTAACC G^GT[ACGT]ACC BstPI 3286 GGTAACC G^GT[ACGT]ACC Bsu36I 1638 CCTTAGG CC^T[ACGT]AGG DraIII 2134 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1554 GACACGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 4964 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1638 CCTTAGG CC^T[ACGT]AGG EcoO65I 3286 GGTAACC G^GT[ACGT]ACC HgaI 18 GCGTC GACGC(5/10) HindIII 4884 AAGCTT A^AGCTT KasI 149 GGCGCC G^GCGCC KspI 3800 CCGCGG CCGC^GG MfeI 2524 CAATTG C^AATTG MluNI 10 TGGCCA TGG^CCA MscI 10 TGGCCA TGG^CCA MunI 2524 CAATTG C^AATTG NarI 149 GGCGCC GG^CGCC PaeR7I 2719 CTCGAG C^TCGAG PflFI 2213 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2938 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 513 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 2928 TCATGA T^CATGA SacII 3800 CCGCGG CCGC^GG SalI 4429 GTCGAC G^TCGAC SfoI 149 GGCGCC GGC^GCC SnaBI 1394 TACGTA TAC^GTA SphI 2971 GCATGC GCATG^C Tth111I 2213 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2938 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 2719 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 1379 GTCTAC GT^[AC][GT]AC 4429 GTCGAC AgeI 124 ACCGGT A^CCGGT 3290 ACCGGT AseI 3338 ATTAAT AT^TAAT 5173 ATTAAT AsnI 3338 ATTAAT AT^TAAT 5173 ATTAAT BamHI 341 GGATCC G^GATCC 772 GGATCC BbsI 702 GAAGAC GAAGAC(2/6) 4189 GTCTTC BcgI 4431 CGACCTCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4392 GCACTGCGCTCG BlpI 3756 GCTAAGC GC^T[ACGT]AGC 5072 GCTCAGC Bpu1102I 3756 GCTAAGC GC^T[ACGT]AGC 5072 GCTCAGC BpuAI 702 GAAGAC GAAGAC(2/6) 4189 GTCTTC BsaAI 1394 TACGTA [CT]AC^GT[GA] 3696 CACGTA BsaWI 124 ACCGGT [AT]^CCGG[AT] 3290 ACCGGT BstAPI 272 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 446 GCAGGCGCTGC CelII 3756 GCTAAGC GC^T[ACGT]AGC 5072 GCTCAGC NaeI 1069 GCCGGC GCC^GGC 3615 GCCGGC NgoMIV 1069 GCCGGC G^CCGGC 3615 GCCGGC PinAI 124 ACCGGT A^CCGGT 3290 ACCGGT PpuMI 1561 AGGTCCT [GA]G^G[AT]CC[CT] 2601 AGGTCCT PshBI 3338 ATTAAT AT^TAAT 5173 ATTAAT SacI 1462 GAGCTC GAGCT^C 2401 GAGCTC SapI 1459 GAAGAGC GCTCTTC(1/4) 1493 GCTCTTC ScaI 2302 AGTACT AGT^ACT 2490 AGTACT ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbvCI BbvI BcnI BfaI BfrI BglI BglII BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI