##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AccIII AclI AflIII Alw44I
Aor51HI ApaLI AscI Asp700I Asp718I
AviII BbrPI BclI BsaBI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssSI Bst1107I BstBI
BstXI BstZ17I ClaI CpoI EagI
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI HpaI
KpnI MamI MluI MroI NdeI
NheI NotI NruI NsiI NspV
PacI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SbfI SexAI SfiI
SfuI SgrAI SpeI Sse8387I SspBI
SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 3537 GACGTC GACGT^C
AhdI 4964 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1638 CCTTAGG CC^T[ACGT]AGG
ApaI 1437 GGGCCC GGGCC^C
AspEI 4964 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2213 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1209 CCTAGG C^CTAGG
BalI 10 TGGCCA TGG^CCA
BbeI 149 GGCGCC GGCGC^C
BciVI 976 GGATAC GTATCC(6/5)
BlnI 1209 CCTAGG C^CTAGG
BsiEI 122 CGACCG CG[GA][CT]^CG
BspHI 2928 TCATGA T^CATGA
BssHII 174 GCGCGC G^CGCGC
BstEII 3286 GGTAACC G^GT[ACGT]ACC
BstPI 3286 GGTAACC G^GT[ACGT]ACC
Bsu36I 1638 CCTTAGG CC^T[ACGT]AGG
DraIII 2134 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1554 GACACGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4964 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1638 CCTTAGG CC^T[ACGT]AGG
EcoO65I 3286 GGTAACC G^GT[ACGT]ACC
HgaI 18 GCGTC GACGC(5/10)
HindIII 4884 AAGCTT A^AGCTT
KasI 149 GGCGCC G^GCGCC
KspI 3800 CCGCGG CCGC^GG
MfeI 2524 CAATTG C^AATTG
MluNI 10 TGGCCA TGG^CCA
MscI 10 TGGCCA TGG^CCA
MunI 2524 CAATTG C^AATTG
NarI 149 GGCGCC GG^CGCC
PaeR7I 2719 CTCGAG C^TCGAG
PflFI 2213 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2938 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 513 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2928 TCATGA T^CATGA
SacII 3800 CCGCGG CCGC^GG
SalI 4429 GTCGAC G^TCGAC
SfoI 149 GGCGCC GGC^GCC
SnaBI 1394 TACGTA TAC^GTA
SphI 2971 GCATGC GCATG^C
Tth111I 2213 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2938 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2719 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 1379 GTCTAC GT^[AC][GT]AC
4429 GTCGAC
AgeI 124 ACCGGT A^CCGGT
3290 ACCGGT
AseI 3338 ATTAAT AT^TAAT
5173 ATTAAT
AsnI 3338 ATTAAT AT^TAAT
5173 ATTAAT
BamHI 341 GGATCC G^GATCC
772 GGATCC
BbsI 702 GAAGAC GAAGAC(2/6)
4189 GTCTTC
BcgI 4431 CGACCTCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4392 GCACTGCGCTCG
BlpI 3756 GCTAAGC GC^T[ACGT]AGC
5072 GCTCAGC
Bpu1102I 3756 GCTAAGC GC^T[ACGT]AGC
5072 GCTCAGC
BpuAI 702 GAAGAC GAAGAC(2/6)
4189 GTCTTC
BsaAI 1394 TACGTA [CT]AC^GT[GA]
3696 CACGTA
BsaWI 124 ACCGGT [AT]^CCGG[AT]
3290 ACCGGT
BstAPI 272 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
446 GCAGGCGCTGC
CelII 3756 GCTAAGC GC^T[ACGT]AGC
5072 GCTCAGC
NaeI 1069 GCCGGC GCC^GGC
3615 GCCGGC
NgoMIV 1069 GCCGGC G^CCGGC
3615 GCCGGC
PinAI 124 ACCGGT A^CCGGT
3290 ACCGGT
PpuMI 1561 AGGTCCT [GA]G^G[AT]CC[CT]
2601 AGGTCCT
PshBI 3338 ATTAAT AT^TAAT
5173 ATTAAT
SacI 1462 GAGCTC GAGCT^C
2401 GAGCTC
SapI 1459 GAAGAGC GCTCTTC(1/4)
1493 GCTCTTC
ScaI 2302 AGTACT AGT^ACT
2490 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BanI BanII
BbvCI BbvI BcnI BfaI BfrI
BglI BglII BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI