##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AscI Asp700I BbeI BbrPI
BfrI BglII BsaAI BseAI BsiWI
BspDI BspEI BspLU11I BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
EcoRI EcoRV HpaI KasI KspI
MluI MroI NarI NdeI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI ScaI SfiI SfoI
SfuI SgrAI SnaBI SphI SspI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 5823 GTAGAC GT^[AC][GT]AC
AhdI 6618 GACTCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 510 ATTAAT AT^TAAT
AsnI 510 ATTAAT AT^TAAT
AspEI 6618 GACTCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3786 CCTAGG C^CTAGG
BbsI 1277 GAAGAC GAAGAC(2/6)
BciVI 5931 GGATAC GTATCC(6/5)
BlnI 3786 CCTAGG C^CTAGG
BpuAI 1277 GAAGAC GAAGAC(2/6)
BsaBI 6093 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 794 TGTACA T^GTACA
BspHI 4002 TCATGA T^CATGA
BsrBI 3426 GAGCGG CCGCTC(-3/-3)
BsrGI 794 TGTACA T^GTACA
DraI 4796 TTTAAA TTT^AAA
DraIII 3614 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 6618 GACTCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2693 CCTGGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 6094 ATGCAT ATGCA^T
FseI 5223 GGCCGGCC GGCCGG^CC
MamI 6093 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 6086 CAATTG C^AATTG
MunI 6086 CAATTG C^AATTG
NaeI 5224 GCCGGC GCC^GGC
NgoMIV 5224 GCCGGC G^CCGGC
NsiI 6094 ATGCAT ATGCA^T
PshAI 5532 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 510 ATTAAT AT^TAAT
RcaI 4002 TCATGA T^CATGA
SacI 3072 GAGCTC GAGCT^C
SbfI 327 CCTGCAGG CCTGCA^GG
SpeI 5 ACTAGT A^CTAGT
Sse8387I 327 CCTGCAGG CCTGCA^GG
SspBI 794 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 2427 AAATTC [GA]^AATT[CT]
3357 GAATTT
Alw44I 2287 GTGCAC G^TGCAC
5376 GTGCAC
Aor51HI 4738 AGCGCT AGC^GCT
4874 AGCGCT
ApaLI 2287 GTGCAC G^TGCAC
5376 GTGCAC
ApoI 2427 AAATTC [GA]^AATT[CT]
3357 GAATTT
AspI 1379 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2811 GACCTTGTC
AviII 920 TGCGCA TGC^GCA
4750 TGCGCA
BbvCI 5710 CCTCAGC CCTCAGC(-5/-2)
5675 GCTGAGG
BcgI 5446 CGAAGGACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2077 GCAGCCCCTTCG
BclI 899 TGATCA T^GATCA
2049 TGATCA
BsaI 4926 GGTCTC GGTCTC(1/5)
6194 GGTCTC
BsiEI 5222 CGGCCG CG[GA][CT]^CG
5226 CGGCCG
BsmBI 5078 CGTCTC CGTCTC(1/5)
5436 GAGACG
BsrFI 1925 ACCGGC [GA]^CCGG[CT]
5224 GCCGGC
BstAPI 923 GCATCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2528 GCAGGCCATGC
BstEII 5019 GGTTACC G^GT[ACGT]ACC
6114 GGTAACC
BstPI 5019 GGTTACC G^GT[ACGT]ACC
6114 GGTAACC
Cfr10I 1925 ACCGGC [GA]^CCGG[CT]
5224 GCCGGC
DrdI 1696 GACTCTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6186 GACAGCTGGGTC
EagI 5222 CGGCCG C^GGCCG
5226 CGGCCG
EclXI 5222 CGGCCG C^GGCCG
5226 CGGCCG
Eco47III 4738 AGCGCT AGC^GCT
4874 AGCGCT
Eco52I 5222 CGGCCG C^GGCCG
5226 CGGCCG
EcoO65I 5019 GGTTACC G^GT[ACGT]ACC
6114 GGTAACC
FbaI 899 TGATCA T^GATCA
2049 TGATCA
FspI 920 TGCGCA TGC^GCA
4750 TGCGCA
HindIII 3209 AAGCTT A^AGCTT
5900 AAGCTT
NcoI 1737 CCATGG C^CATGG
5524 CCATGG
NheI 634 GCTAGC G^CTAGC
2930 GCTAGC
PaeR7I 2590 CTCGAG C^TCGAG
6649 CTCGAG
PflFI 1379 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2811 GACCTTGTC
SapI 1685 GCTCTTC GCTCTTC(1/4)
6145 GCTCTTC
SmlI 2590 CTCGAG C^T[CT][GA]AG
6649 CTCGAG
Tth111I 1379 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2811 GACCTTGTC
XhoI 2590 CTCGAG C^TCGAG
6649 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
Asp718I AspHI AvaI AvaII BalI
BamHI BanI BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmaI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI