################################## # NO CUT ENZYMES ( 37 enzymes) # ################################## Acc65I AflII AgeI AscI Asp718I AvrII BfrI BlnI BspLU11I BssHII BstBI EcoRV EcoT22I FseI HpaI KpnI KspI MfeI MluI MunI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PvuI SacII SalI SbfI SfuI SnaBI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AclI 4248 AACGTT AA^CGTT AhdI 378 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 5129 CCTGAGG CC^T[ACGT]AGG Aor51HI 469 AGCGCT AGC^GCT AspEI 378 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BciVI 6225 GTATCC GTATCC(6/5) BsiWI 3193 CGTACG C^GTACG BsmBI 4095 GAGACG CGTCTC(1/5) BspDI 5902 ATCGAT AT^CGAT BspHI 3385 TCATGA T^CATGA Bsu36I 5129 CCTGAGG CC^T[ACGT]AGG ClaI 5902 ATCGAT AT^CGAT CpoI 2554 CGGTCCG CG^G[AT]CCG DraI 5567 TTTAAA TTT^AAA DraIII 5772 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 258 GACTCCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 378 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 469 AGCGCT AGC^GCT Eco81I 5129 CCTGAGG CC^T[ACGT]AGG EcoRI 3891 GAATTC G^AATTC HindIII 6701 AAGCTT A^AGCTT NdeI 5846 CATATG CA^TATG Psp1406I 4248 AACGTT AA^CGTT RcaI 3385 TCATGA T^CATGA RsrII 2554 CGGTCCG CG^G[AT]CCG SapI 3395 GCTCTTC GCTCTTC(1/4) ScaI 1690 AGTACT AGT^ACT SexAI 1249 ACCAGGT A^CC[AT]GGT SfiI 2909 GGCCAAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 25 CGCCGGCG C[GA]^CCGG[CT]G SpeI 5712 ACTAGT A^CTAGT SphI 1507 GCATGC GCATG^C SspI 5348 AATATT AAT^ATT XbaI 6811 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AatII 1929 GACGTC GACGT^C 4097 GACGTC AccIII 3457 TCCGGA T^CCGGA 3895 TCCGGA AflIII 3130 ACACGT A^C[GA][CT]GT 6347 ACGTGT Alw44I 587 GTGCAC G^TGCAC 1670 GTGCAC ApaLI 587 GTGCAC G^TGCAC 1670 GTGCAC AseI 4619 ATTAAT AT^TAAT 5411 ATTAAT AsnI 4619 ATTAAT AT^TAAT 5411 ATTAAT AspI 297 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 4115 GACCCTGTC BamHI 2671 GGATCC G^GATCC 3784 GGATCC BbrPI 1299 CACGTG CAC^GTG 6346 CACGTG BcgI 905 CGAAGGATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2846 CGACGGGCCTGC BglII 2716 AGATCT A^GATCT 6339 AGATCT BlpI 106 GCTCAGC GC^T[ACGT]AGC 5766 GCTAAGC Bpu1102I 106 GCTCAGC GC^T[ACGT]AGC 5766 GCTAAGC BsaBI 3506 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5444 GATGAGGATC BseAI 3457 TCCGGA T^CCGGA 3895 TCCGGA BsmI 6560 GAATGC GAATGC(1/-1) 3338 GCATTC BspEI 3457 TCCGGA T^CCGGA 3895 TCCGGA BssSI 804 CTCGTG CACGAG(-5/-1) 2311 CTCGTG Bst1107I 4741 GTATAC GTA^TAC 4759 GTATAC BstEII 1167 GGTGACC G^GT[ACGT]ACC 6256 GGTGACC BstPI 1167 GGTGACC G^GT[ACGT]ACC 6256 GGTGACC BstZ17I 4741 GTATAC GTA^TAC 4759 GTATAC CelII 106 GCTCAGC GC^T[ACGT]AGC 5766 GCTAAGC EagI 388 CGGCCG C^GGCCG 3197 CGGCCG EclXI 388 CGGCCG C^GGCCG 3197 CGGCCG Eco52I 388 CGGCCG C^GGCCG 3197 CGGCCG EcoO65I 1167 GGTGACC G^GT[ACGT]ACC 6256 GGTGACC MamI 3506 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5444 GATGAGGATC MroI 3457 TCCGGA T^CCGGA 3895 TCCGGA PaeR7I 2646 CTCGAG C^TCGAG 3974 CTCGAG PflFI 297 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 4115 GACCCTGTC PmaCI 1299 CACGTG CAC^GTG 6346 CACGTG PmlI 1299 CACGTG CAC^GTG 6346 CACGTG PshBI 4619 ATTAAT AT^TAAT 5411 ATTAAT SmaI 88 CCCGGG CCC^GGG 373 CCCGGG Tth111I 297 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 4115 GACCCTGTC XhoI 2646 CTCGAG C^TCGAG 3974 CTCGAG XmaCI 88 CCCGGG C^CCGGG 373 CCCGGG XmaI 88 CCCGGG C^CCGGG 373 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 147 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI Asp700I AspHI AvaI AvaII AviII BalI BanI BanII BbeI BbsI BbvCI BbvI BclI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI