##################################
# NO CUT ENZYMES ( 37 enzymes) #
##################################
Acc65I AflII AgeI AscI Asp718I
AvrII BfrI BlnI BspLU11I BssHII
BstBI EcoRV EcoT22I FseI HpaI
KpnI KspI MfeI MluI MunI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshAI PvuI
SacII SalI SbfI SfuI SnaBI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AclI 4248 AACGTT AA^CGTT
AhdI 378 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 5129 CCTGAGG CC^T[ACGT]AGG
Aor51HI 469 AGCGCT AGC^GCT
AspEI 378 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 6225 GTATCC GTATCC(6/5)
BsiWI 3193 CGTACG C^GTACG
BsmBI 4095 GAGACG CGTCTC(1/5)
BspDI 5902 ATCGAT AT^CGAT
BspHI 3385 TCATGA T^CATGA
Bsu36I 5129 CCTGAGG CC^T[ACGT]AGG
ClaI 5902 ATCGAT AT^CGAT
CpoI 2554 CGGTCCG CG^G[AT]CCG
DraI 5567 TTTAAA TTT^AAA
DraIII 5772 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 258 GACTCCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 378 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 469 AGCGCT AGC^GCT
Eco81I 5129 CCTGAGG CC^T[ACGT]AGG
EcoRI 3891 GAATTC G^AATTC
HindIII 6701 AAGCTT A^AGCTT
NdeI 5846 CATATG CA^TATG
Psp1406I 4248 AACGTT AA^CGTT
RcaI 3385 TCATGA T^CATGA
RsrII 2554 CGGTCCG CG^G[AT]CCG
SapI 3395 GCTCTTC GCTCTTC(1/4)
ScaI 1690 AGTACT AGT^ACT
SexAI 1249 ACCAGGT A^CC[AT]GGT
SfiI 2909 GGCCAAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 25 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 5712 ACTAGT A^CTAGT
SphI 1507 GCATGC GCATG^C
SspI 5348 AATATT AAT^ATT
XbaI 6811 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1929 GACGTC GACGT^C
4097 GACGTC
AccIII 3457 TCCGGA T^CCGGA
3895 TCCGGA
AflIII 3130 ACACGT A^C[GA][CT]GT
6347 ACGTGT
Alw44I 587 GTGCAC G^TGCAC
1670 GTGCAC
ApaLI 587 GTGCAC G^TGCAC
1670 GTGCAC
AseI 4619 ATTAAT AT^TAAT
5411 ATTAAT
AsnI 4619 ATTAAT AT^TAAT
5411 ATTAAT
AspI 297 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4115 GACCCTGTC
BamHI 2671 GGATCC G^GATCC
3784 GGATCC
BbrPI 1299 CACGTG CAC^GTG
6346 CACGTG
BcgI 905 CGAAGGATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2846 CGACGGGCCTGC
BglII 2716 AGATCT A^GATCT
6339 AGATCT
BlpI 106 GCTCAGC GC^T[ACGT]AGC
5766 GCTAAGC
Bpu1102I 106 GCTCAGC GC^T[ACGT]AGC
5766 GCTAAGC
BsaBI 3506 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5444 GATGAGGATC
BseAI 3457 TCCGGA T^CCGGA
3895 TCCGGA
BsmI 6560 GAATGC GAATGC(1/-1)
3338 GCATTC
BspEI 3457 TCCGGA T^CCGGA
3895 TCCGGA
BssSI 804 CTCGTG CACGAG(-5/-1)
2311 CTCGTG
Bst1107I 4741 GTATAC GTA^TAC
4759 GTATAC
BstEII 1167 GGTGACC G^GT[ACGT]ACC
6256 GGTGACC
BstPI 1167 GGTGACC G^GT[ACGT]ACC
6256 GGTGACC
BstZ17I 4741 GTATAC GTA^TAC
4759 GTATAC
CelII 106 GCTCAGC GC^T[ACGT]AGC
5766 GCTAAGC
EagI 388 CGGCCG C^GGCCG
3197 CGGCCG
EclXI 388 CGGCCG C^GGCCG
3197 CGGCCG
Eco52I 388 CGGCCG C^GGCCG
3197 CGGCCG
EcoO65I 1167 GGTGACC G^GT[ACGT]ACC
6256 GGTGACC
MamI 3506 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5444 GATGAGGATC
MroI 3457 TCCGGA T^CCGGA
3895 TCCGGA
PaeR7I 2646 CTCGAG C^TCGAG
3974 CTCGAG
PflFI 297 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4115 GACCCTGTC
PmaCI 1299 CACGTG CAC^GTG
6346 CACGTG
PmlI 1299 CACGTG CAC^GTG
6346 CACGTG
PshBI 4619 ATTAAT AT^TAAT
5411 ATTAAT
SmaI 88 CCCGGG CCC^GGG
373 CCCGGG
Tth111I 297 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4115 GACCCTGTC
XhoI 2646 CTCGAG C^TCGAG
3974 CTCGAG
XmaCI 88 CCCGGG C^CCGGG
373 CCCGGG
XmaI 88 CCCGGG C^CCGGG
373 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApaI
ApoI Asp700I AspHI AvaI AvaII
AviII BalI BanI BanII BbeI
BbsI BbvCI BbvI BclI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI