################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## Acc65I AccI AclI AflII AgeI AhdI Alw44I ApaLI AscI Asp718I AspEI BamHI BbrPI BfrI BglII BsaAI BsiEI BsiWI Bsp1407I BspDI BspHI BsrGI BssHII Bst1107I BstBI BstYI BstZ17I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoRV FseI HindIII HpaI KpnI KspI MfeI MflI MluI MunI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SexAI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AatII 3690 GACGTC GACGT^C AccIII 3951 TCCGGA T^CCGGA AflIII 4941 ACATGT A^C[GA][CT]GT AseI 1726 ATTAAT AT^TAAT AsnI 1726 ATTAAT AT^TAAT Asp700I 685 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BcgI 213 GCATGAAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2111 GTATCC GTATCC(6/5) BsaBI 2764 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 3951 TCCGGA [AT]^CCGG[AT] BseAI 3951 TCCGGA T^CCGGA BspEI 3951 TCCGGA T^CCGGA BspLU11I 4941 ACATGT A^CATGT BssSI 777 CACGAG CACGAG(-5/-1) BstEII 343 GGTCACC G^GT[ACGT]ACC BstPI 343 GGTCACC G^GT[ACGT]ACC DraI 5929 TTTAAA TTT^AAA EcoO65I 343 GGTCACC G^GT[ACGT]ACC EcoT22I 687 ATGCAT ATGCA^T HincII 1487 GTCAAC GT[CT]^[GA]AC HindII 1487 GTCAAC GT[CT]^[GA]AC MamI 2764 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 3951 TCCGGA T^CCGGA NsiI 687 ATGCAT ATGCA^T PshBI 1726 ATTAAT AT^TAAT SacI 1923 GAGCTC GAGCT^C SbfI 2966 CCTGCAGG CCTGCA^GG SfiI 2893 GGCCAGGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 2966 CCTGCAGG CCTGCA^GG XmnI 685 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccII 3813 CGCG CG^CG 4591 CGCG AviII 4899 TGCGCA TGC^GCA 4969 TGCGCA AvrII 4202 CCTAGG C^CTAGG 5139 CCTAGG BbeI 4448 GGCGCC GGCGC^C 5073 GGCGCC BclI 1622 TGATCA T^GATCA 4060 TGATCA BlnI 4202 CCTAGG C^CTAGG 5139 CCTAGG BsmBI 3723 GAGACG CGTCTC(1/5) 4570 GAGACG BsrDI 1422 CATTGC GCAATG(2/0) 2213 CATTGC BstUI 3813 CGCG CG^CG 4591 CGCG FbaI 1622 TGATCA T^GATCA 4060 TGATCA FspI 4899 TGCGCA TGC^GCA 4969 TGCGCA KasI 4448 GGCGCC G^GCGCC 5073 GGCGCC MvnI 3813 CGCG CG^CG 4591 CGCG NaeI 941 GCCGGC GCC^GGC 3182 GCCGGC NarI 4448 GGCGCC GG^CGCC 5073 GGCGCC NcoI 5360 CCATGG C^CATGG 5573 CCATGG NgoMIV 941 GCCGGC G^CCGGC 3182 GCCGGC PshAI 337 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1037 GACTGGGGTC SfoI 4448 GGCGCC GGC^GCC 5073 GGCGCC SphI 4141 GCATGC GCATG^C 4575 GCATGC TaqI 5441 TCGA T^CGA 5770 TCGA TthHB8I 5441 TCGA T^CGA 5770 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI Aor51HI ApaI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I Van91I XcmI XmaCI XmaI