##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI Alw44I ApaLI AscI Asp718I
AspEI BamHI BbrPI BfrI BglII
BsaAI BsiEI BsiWI Bsp1407I BspDI
BspHI BsrGI BssHII Bst1107I BstBI
BstYI BstZ17I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoRV FseI HindIII HpaI KpnI
KspI MfeI MflI MluI MunI
NdeI NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacII SalI SapI ScaI SexAI
SfuI SgrAI SnaBI SpeI SspBI
SspI SwaI XbaI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AatII 3690 GACGTC GACGT^C
AccIII 3951 TCCGGA T^CCGGA
AflIII 4941 ACATGT A^C[GA][CT]GT
AseI 1726 ATTAAT AT^TAAT
AsnI 1726 ATTAAT AT^TAAT
Asp700I 685 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 213 GCATGAAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2111 GTATCC GTATCC(6/5)
BsaBI 2764 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3951 TCCGGA [AT]^CCGG[AT]
BseAI 3951 TCCGGA T^CCGGA
BspEI 3951 TCCGGA T^CCGGA
BspLU11I 4941 ACATGT A^CATGT
BssSI 777 CACGAG CACGAG(-5/-1)
BstEII 343 GGTCACC G^GT[ACGT]ACC
BstPI 343 GGTCACC G^GT[ACGT]ACC
DraI 5929 TTTAAA TTT^AAA
EcoO65I 343 GGTCACC G^GT[ACGT]ACC
EcoT22I 687 ATGCAT ATGCA^T
HincII 1487 GTCAAC GT[CT]^[GA]AC
HindII 1487 GTCAAC GT[CT]^[GA]AC
MamI 2764 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3951 TCCGGA T^CCGGA
NsiI 687 ATGCAT ATGCA^T
PshBI 1726 ATTAAT AT^TAAT
SacI 1923 GAGCTC GAGCT^C
SbfI 2966 CCTGCAGG CCTGCA^GG
SfiI 2893 GGCCAGGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 2966 CCTGCAGG CCTGCA^GG
XmnI 685 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 3813 CGCG CG^CG
4591 CGCG
AviII 4899 TGCGCA TGC^GCA
4969 TGCGCA
AvrII 4202 CCTAGG C^CTAGG
5139 CCTAGG
BbeI 4448 GGCGCC GGCGC^C
5073 GGCGCC
BclI 1622 TGATCA T^GATCA
4060 TGATCA
BlnI 4202 CCTAGG C^CTAGG
5139 CCTAGG
BsmBI 3723 GAGACG CGTCTC(1/5)
4570 GAGACG
BsrDI 1422 CATTGC GCAATG(2/0)
2213 CATTGC
BstUI 3813 CGCG CG^CG
4591 CGCG
FbaI 1622 TGATCA T^GATCA
4060 TGATCA
FspI 4899 TGCGCA TGC^GCA
4969 TGCGCA
KasI 4448 GGCGCC G^GCGCC
5073 GGCGCC
MvnI 3813 CGCG CG^CG
4591 CGCG
NaeI 941 GCCGGC GCC^GGC
3182 GCCGGC
NarI 4448 GGCGCC GG^CGCC
5073 GGCGCC
NcoI 5360 CCATGG C^CATGG
5573 CCATGG
NgoMIV 941 GCCGGC G^CCGGC
3182 GCCGGC
PshAI 337 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1037 GACTGGGGTC
SfoI 4448 GGCGCC GGC^GCC
5073 GGCGCC
SphI 4141 GCATGC GCATG^C
4575 GCATGC
TaqI 5441 TCGA T^CGA
5770 TCGA
TthHB8I 5441 TCGA T^CGA
5770 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
ApoI AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstXI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I Van91I XcmI XmaCI
XmaI