##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Aor51HI AscI Asp718I AviII AvrII
BbeI BfrI BlnI BsiWI BspDI
BspHI BssHII Bst1107I BstAPI BstZ17I
ClaI CpoI DrdI Eco47III EcoRV
EcoT22I FseI FspI HaeII HpaI
KasI KpnI KspI NarI NheI
NotI NruI NsiI PacI PinAI
PmeI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SexAI SfoI
SgrAI SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AhdI 1380 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1380 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 6029 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 5085 GGATCC G^GATCC
BglII 425 AGATCT A^GATCT
BspLU11I 2670 ACATGT A^CATGT
BsrBI 1655 CCGCTC CCGCTC(-3/-3)
BssSI 1988 CACGAG CACGAG(-5/-1)
BstEII 2683 GGTCACC G^GT[ACGT]ACC
BstPI 2683 GGTCACC G^GT[ACGT]ACC
EagI 190 CGGCCG C^GGCCG
Eam1105I 1380 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 190 CGGCCG C^GGCCG
Eco52I 190 CGGCCG C^GGCCG
EcoO65I 2683 GGTCACC G^GT[ACGT]ACC
HincII 1644 GTTGAC GT[CT]^[GA]AC
HindII 1644 GTTGAC GT[CT]^[GA]AC
HindIII 2390 AAGCTT A^AGCTT
MfeI 5201 CAATTG C^AATTG
MluI 2756 ACGCGT A^CGCGT
MunI 5201 CAATTG C^AATTG
NdeI 357 CATATG CA^TATG
PaeR7I 436 CTCGAG C^TCGAG
PflFI 6029 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2610 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2234 GAGCTC GAGCT^C
SbfI 4000 CCTGCAGG CCTGCA^GG
SfiI 1854 GGCCTGATAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 128 CCCGGG CCC^GGG
SphI 6152 GCATGC GCATG^C
Sse8387I 4000 CCTGCAGG CCTGCA^GG
Tth111I 6029 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2639 TCTAGA T^CTAGA
XhoI 436 CTCGAG C^TCGAG
XmaCI 128 CCCGGG C^CCGGG
XmaI 128 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 993 GTCTAC GT^[AC][GT]AC
1518 GTAGAC
AccIII 100 TCCGGA T^CCGGA
2321 TCCGGA
AflIII 2670 ACATGT A^C[GA][CT]GT
2756 ACGCGT
Alw44I 3199 GTGCAC G^TGCAC
3665 GTGCAC
ApaLI 3199 GTGCAC G^TGCAC
3665 GTGCAC
Asp700I 1037 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3098 GAAACATTTC
BbrPI 2572 CACGTG CAC^GTG
5622 CACGTG
BciVI 5853 GTATCC GTATCC(6/5)
5973 GTATCC
BclI 418 TGATCA T^GATCA
4394 TGATCA
BlpI 332 GCTGAGC GC^T[ACGT]AGC
4085 GCTAAGC
Bpu1102I 332 GCTGAGC GC^T[ACGT]AGC
4085 GCTAAGC
BsaBI 3245 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4395 GATCAGTATC
BseAI 100 TCCGGA T^CCGGA
2321 TCCGGA
BsiEI 190 CGGCCG CG[GA][CT]^CG
1021 CGGTCG
BsmBI 3704 GAGACG CGTCTC(1/5)
6027 GAGACG
BsmI 2557 GAATGC GAATGC(1/-1)
4734 GAATGC
Bsp1407I 1803 TGTACA T^GTACA
2709 TGTACA
BspEI 100 TCCGGA T^CCGGA
2321 TCCGGA
BsrFI 280 GCCGGC [GA]^CCGG[CT]
2457 GCCGGC
BsrGI 1803 TGTACA T^GTACA
2709 TGTACA
BstBI 3095 TTCGAA TT^CGAA
4260 TTCGAA
CelII 332 GCTGAGC GC^T[ACGT]AGC
4085 GCTAAGC
Cfr10I 280 GCCGGC [GA]^CCGG[CT]
2457 GCCGGC
DraIII 1255 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4883 CACACTGTG
EcoRI 2199 GAATTC G^AATTC
2939 GAATTC
FbaI 418 TGATCA T^GATCA
4394 TGATCA
MamI 3245 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4395 GATCAGTATC
MroI 100 TCCGGA T^CCGGA
2321 TCCGGA
NaeI 280 GCCGGC GCC^GGC
2457 GCCGGC
NgoMIV 280 GCCGGC G^CCGGC
2457 GCCGGC
NspV 3095 TTCGAA TT^CGAA
4260 TTCGAA
PmaCI 2572 CACGTG CAC^GTG
5622 CACGTG
PmlI 2572 CACGTG CAC^GTG
5622 CACGTG
SfuI 3095 TTCGAA TT^CGAA
4260 TTCGAA
SspBI 1803 TGTACA T^GTACA
2709 TGTACA
TfiI 587 GATTC G^A[AT]TC
5557 GAATC
XmnI 1037 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3098 GAAACATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AseI AsnI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PshBI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII