##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AgeI AhdI AscI
Asp718I AspEI AviII AvrII BlnI
BsiEI BsiWI BspDI BsrBI BsrFI
BssHII BstBI Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoRI
FseI FspI HgaI HincII HindII
HpaI KpnI KspI MfeI MluI
MunI NaeI NgoMIV NheI NotI
NruI NspV PacI PinAI PmeI
PvuI RsrII SacI SacII SalI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SwaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 2269 CGCG CG^CG
AccIII 1429 TCCGGA T^CCGGA
AclI 6380 AACGTT AA^CGTT
AcyI 1683 GGCGCC G[GA]^CG[CT]C
AflII 4801 CTTAAG C^TTAAG
AocI 2712 CCTTAGG CC^T[ACGT]AGG
BbeI 1683 GGCGCC GGCGC^C
BfrI 4801 CTTAAG C^TTAAG
BglII 5881 AGATCT A^GATCT
BlpI 306 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 306 GCTCAGC GC^T[ACGT]AGC
BsaBI 829 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1683 GGCGCC G[GA]^CG[CT]C
BseAI 1429 TCCGGA T^CCGGA
BsmBI 1955 CGTCTC CGTCTC(1/5)
BspEI 1429 TCCGGA T^CCGGA
Bst1107I 1465 GTATAC GTA^TAC
BstUI 2269 CGCG CG^CG
BstZ17I 1465 GTATAC GTA^TAC
Bsu36I 2712 CCTTAGG CC^T[ACGT]AGG
CelII 306 GCTCAGC GC^T[ACGT]AGC
DraIII 4327 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2712 CCTTAGG CC^T[ACGT]AGG
EcoRV 5317 GATATC GAT^ATC
Hin1I 1683 GGCGCC G[GA]^CG[CT]C
HindIII 604 AAGCTT A^AGCTT
KasI 1683 GGCGCC G^GCGCC
MamI 829 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1429 TCCGGA T^CCGGA
MvnI 2269 CGCG CG^CG
NarI 1683 GGCGCC GG^CGCC
NcoI 4041 CCATGG C^CATGG
PaeR7I 4668 CTCGAG C^TCGAG
PflMI 3174 CCAATGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 6380 AACGTT AA^CGTT
SapI 6439 GAAGAGC GCTCTTC(1/4)
SfoI 1683 GGCGCC GGC^GCC
SmaI 5422 CCCGGG CCC^GGG
Van91I 3174 CCAATGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5884 TCTAGA T^CTAGA
XhoI 4668 CTCGAG C^TCGAG
XmaCI 5422 CCCGGG C^CCGGG
XmaI 5422 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Alw44I 1962 GTGCAC G^TGCAC
4762 GTGCAC
Aor51HI 2970 AGCGCT AGC^GCT
6130 AGCGCT
ApaLI 1962 GTGCAC G^TGCAC
4762 GTGCAC
Asp700I 227 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5713 GAAGATGTTC
AspI 1244 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2006 GACTTTGTC
AvaI 4668 CTCGAG C^[CT]CG[GA]G
5422 CCCGGG
BamHI 1426 GGATCC G^GATCC
1785 GGATCC
BbvCI 2093 CCTCAGC CCTCAGC(-5/-2)
1153 GCTGAGG
BcgI 97 GCAGGCTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4982 GCAAGTACATCG
BciVI 4210 GGATAC GTATCC(6/5)
6004 GGATAC
BclI 2407 TGATCA T^GATCA
6271 TGATCA
BsaWI 1429 TCCGGA [AT]^CCGG[AT]
5564 ACCGGA
BsoBI 4668 CTCGAG C^[CT]CG[GA]G
5422 CCCGGG
BspLU11I 785 ACATGT A^CATGT
4679 ACATGT
BstEII 3025 GGTCACC G^GT[ACGT]ACC
5125 GGTGACC
BstPI 3025 GGTCACC G^GT[ACGT]ACC
5125 GGTGACC
DrdI 5019 GACAGTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5791 GACTGTATAGTC
Eco47III 2970 AGCGCT AGC^GCT
6130 AGCGCT
EcoNI 3685 CCTGTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4199 CCTCGTAGAGG
EcoO65I 3025 GGTCACC G^GT[ACGT]ACC
5125 GGTGACC
EcoT22I 3177 ATGCAT ATGCA^T
5983 ATGCAT
FbaI 2407 TGATCA T^GATCA
6271 TGATCA
NdeI 540 CATATG CA^TATG
3859 CATATG
NsiI 3177 ATGCAT ATGCA^T
5983 ATGCAT
PflFI 1244 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2006 GACTTTGTC
PshAI 2630 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3469 GACTTGAGTC
SbfI 94 CCTGCAGG CCTGCA^GG
4190 CCTGCAGG
Sse8387I 94 CCTGCAGG CCTGCA^GG
4190 CCTGCAGG
Tth111I 1244 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2006 GACTTTGTC
XcmI 1772 CCACAATACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2963 CCAGAGCAGCGCTGG
XmnI 227 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5713 GAAGATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AseI AsnI AspHI AvaII BalI
BanI BanII BbrPI BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspHI
BspMI BsrDI BsrGI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PmaCI PmlI
PpuMI PshBI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SpeI SphI SspBI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII