##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccI AccIII AgeI Alw44I
Aor51HI ApaI ApaLI AscI AspI
AviII BcgI BclI BglI BseAI
BsiEI BsiWI BspDI BspEI BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoT22I FbaI FseI
FspI KspI MluI MroI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfuI SgrAI SnaBI Sse8387I
SspI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 831 GGTACC G^GTACC
AccII 1518 CGCG CG^CG
AflII 2480 CTTAAG C^TTAAG
AhdI 1187 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 3298 ATTAAT AT^TAAT
AsnI 3298 ATTAAT AT^TAAT
Asp718I 831 GGTACC G^GTACC
AspEI 1187 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 4426 GGATCC G^GATCC
BbeI 1377 GGCGCC GGCGC^C
BbrPI 284 CACGTG CAC^GTG
BfrI 2480 CTTAAG C^TTAAG
BglII 4236 AGATCT A^GATCT
BsaAI 284 CACGTG [CT]AC^GT[GA]
BsgI 911 CTGCAC GTGCAG(16/14)
BsmI 5460 GAATGC GAATGC(1/-1)
Bsp1407I 3240 TGTACA T^GTACA
BsrBI 997 CCGCTC CCGCTC(-3/-3)
BsrFI 1081 GCCGGC [GA]^CCGG[CT]
BsrGI 3240 TGTACA T^GTACA
BstEII 757 GGTCACC G^GT[ACGT]ACC
BstPI 757 GGTCACC G^GT[ACGT]ACC
BstUI 1518 CGCG CG^CG
Cfr10I 1081 GCCGGC [GA]^CCGG[CT]
DraIII 372 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1187 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 757 GGTCACC G^GT[ACGT]ACC
EcoRV 3374 GATATC GAT^ATC
HindIII 3831 AAGCTT A^AGCTT
HpaI 5632 GTTAAC GTT^AAC
KasI 1377 GGCGCC G^GCGCC
KpnI 831 GGTACC GGTAC^C
MfeI 394 CAATTG C^AATTG
MunI 394 CAATTG C^AATTG
MvnI 1518 CGCG CG^CG
NaeI 1081 GCCGGC GCC^GGC
NarI 1377 GGCGCC GG^CGCC
NcoI 729 CCATGG C^CATGG
NdeI 4075 CATATG CA^TATG
NgoMIV 1081 GCCGGC G^CCGGC
NheI 2397 GCTAGC G^CTAGC
PmaCI 284 CACGTG CAC^GTG
PmlI 284 CACGTG CAC^GTG
PshAI 5498 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3298 ATTAAT AT^TAAT
SacI 4173 GAGCTC GAGCT^C
SapI 3030 GCTCTTC GCTCTTC(1/4)
SexAI 222 ACCTGGT A^CC[AT]GGT
SfoI 1377 GGCGCC GGC^GCC
SmaI 1674 CCCGGG CCC^GGG
SpeI 1950 ACTAGT A^CTAGT
SspBI 3240 TGTACA T^GTACA
XmaCI 1674 CCCGGG C^CCGGG
XmaI 1674 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AclI 333 AACGTT AA^CGTT
854 AACGTT
AcyI 1078 GACGCC G[GA]^CG[CT]C
1377 GGCGCC
AvaI 1674 CCCGGG C^[CT]CG[GA]G
2315 CCCGAG
BbsI 6 GAAGAC GAAGAC(2/6)
4509 GTCTTC
BpuAI 6 GAAGAC GAAGAC(2/6)
4509 GTCTTC
BsaHI 1078 GACGCC G[GA]^CG[CT]C
1377 GGCGCC
BsmBI 1076 GAGACG CGTCTC(1/5)
1514 GAGACG
BsoBI 1674 CCCGGG C^[CT]CG[GA]G
2315 CCCGAG
BspHI 571 TCATGA T^CATGA
3910 TCATGA
DsaI 729 CCATGG C^C[GA][CT]GG
2201 CCGTGG
EcoNI 64 CCTGTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1025 CCTCAGCGAGG
EcoRI 4293 GAATTC G^AATTC
5678 GAATTC
HaeII 549 AGCGCC [GA]GCGC^[CT]
1377 GGCGCC
Hin1I 1078 GACGCC G[GA]^CG[CT]C
1377 GGCGCC
Psp1406I 333 AACGTT AA^CGTT
854 AACGTT
RcaI 571 TCATGA T^CATGA
3910 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaII AvrII BalI BanI
BanII BbvCI BbvI BciVI BcnI
BfaI BlnI BlpI BmyI BpmI
Bpu1102I BsaBI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI