##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccIII AgeI Aor51HI AscI AspI
AviII AvrII BbrPI BcgI BlnI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrFI BssHII Bst1107I BstAPI
BstBI BstZ17I Cfr10I ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoT22I FauI FseI FspI
KspI MroI NaeI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PvuI RsrII SacII SalI SapI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SphI SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 4108 GACGTC GACGT^C
Acc65I 2397 GGTACC G^GTACC
AccI 4026 GTCTAC GT^[AC][GT]AC
AccII 149 CGCG CG^CG
AflII 4200 CTTAAG C^TTAAG
Alw44I 4042 GTGCAC G^TGCAC
AocI 787 CCTCAGG CC^T[ACGT]AGG
ApaI 3307 GGGCCC GGGCC^C
ApaLI 4042 GTGCAC G^TGCAC
AseI 321 ATTAAT AT^TAAT
AsnI 321 ATTAAT AT^TAAT
Asp718I 2397 GGTACC G^GTACC
BalI 2434 TGGCCA TGG^CCA
BbeI 137 GGCGCC GGCGC^C
BbvCI 1948 CCTCAGC CCTCAGC(-5/-2)
BfrI 4200 CTTAAG C^TTAAG
BlpI 3742 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3742 GCTTAGC GC^T[ACGT]AGC
BsaBI 3348 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 447 GAGACC GGTCTC(1/5)
Bsp1407I 4243 TGTACA T^GTACA
BspLU11I 4837 ACATGT A^CATGT
BspMI 4573 ACCTGC ACCTGC(4/8)
BsrBI 3689 GAGCGG CCGCTC(-3/-3)
BsrGI 4243 TGTACA T^GTACA
BstEII 3394 GGTTACC G^GT[ACGT]ACC
BstPI 3394 GGTTACC G^GT[ACGT]ACC
BstUI 149 CGCG CG^CG
Bsu36I 787 CCTCAGG CC^T[ACGT]AGG
CelII 3742 GCTTAGC GC^T[ACGT]AGC
EaeI 2434 TGGCCA [CT]^GGCC[GA]
Eco81I 787 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3394 GGTTACC G^GT[ACGT]ACC
EcoRI 4090 GAATTC G^AATTC
EcoRV 546 GATATC GAT^ATC
HaeII 137 GGCGCC [GA]GCGC^[CT]
HindIII 4999 AAGCTT A^AGCTT
HpaI 3968 GTTAAC GTT^AAC
KasI 137 GGCGCC G^GCGCC
KpnI 2397 GGTACC GGTAC^C
MamI 3348 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 148 ACGCGT A^CGCGT
MluNI 2434 TGGCCA TGG^CCA
MscI 2434 TGGCCA TGG^CCA
MvnI 149 CGCG CG^CG
NarI 137 GGCGCC GG^CGCC
NcoI 1282 CCATGG C^CATGG
PaeR7I 4408 CTCGAG C^TCGAG
PflMI 3170 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4980 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 321 ATTAAT AT^TAAT
SacI 96 GAGCTC GAGCT^C
SbfI 631 CCTGCAGG CCTGCA^GG
SfoI 137 GGCGCC GGC^GCC
SpeI 1095 ACTAGT A^CTAGT
Sse8387I 631 CCTGCAGG CCTGCA^GG
SspBI 4243 TGTACA T^GTACA
SspI 1581 AATATT AAT^ATT
Van91I 3170 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 4408 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AclI 1162 AACGTT AA^CGTT
3231 AACGTT
AhdI 1039 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1986 GACAGCCAGTC
AlwNI 3330 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
4898 CAGAGTCTG
AspEI 1039 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1986 GACAGCCAGTC
BamHI 999 GGATCC G^GATCC
1974 GGATCC
BclI 253 TGATCA T^GATCA
4582 TGATCA
BglI 2362 GCCTCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5008 GCCGAGATGGC
BglII 1872 AGATCT A^GATCT
3467 AGATCT
BsaAI 3045 TACGTG [CT]AC^GT[GA]
5299 CACGTA
BsgI 607 CTGCAC GTGCAG(16/14)
904 CTGCAC
BsmI 3031 GAATGC GAATGC(1/-1)
3423 GAATGC
BsrDI 294 GCAATG GCAATG(2/0)
3622 GCAATG
BssSI 2088 CACGAG CACGAG(-5/-1)
3904 CTCGTG
CfoI 138 GCGC GCG^C
5245 GCGC
DsaI 81 CCACGG C^C[GA][CT]GG
1282 CCATGG
Eam1105I 1039 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1986 GACAGCCAGTC
FbaI 253 TGATCA T^GATCA
4582 TGATCA
HhaI 138 GCGC GCG^C
5245 GCGC
HinP1I 138 GCGC G^CGC
5245 GCGC
NheI 1466 GCTAGC G^CTAGC
2240 GCTAGC
NspI 4514 GCATGT [GA]CATG^[CT]
4837 ACATGT
Psp1406I 1162 AACGTT AA^CGTT
3231 AACGTT
PstI 632 CTGCAG CTGCA^G
2866 CTGCAG
StuI 850 AGGCCT AGG^CCT
2369 AGGCCT
XcmI 38 CCATTTTTCCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3113 CCAGAGAGAAATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbsI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI Hin1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MspI MunI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII XmnI