##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AclI AhdI
Aor51HI AscI Asp718I AspEI AspI
AviII BalI BbrPI BglI BlpI
Bpu1102I BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BssHII BssSI BstBI
CelII ClaI CpoI DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV FseI FspI HpaI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI NheI NotI NruI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII RsrII SacII SalI SbfI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 85 CGCG CG^CG
AcyI 1182 GGCGCC G[GA]^CG[CT]C
AgeI 1499 ACCGGT A^CCGGT
Alw44I 1815 GTGCAC G^TGCAC
AlwNI 2018 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 4569 GGGCCC GGGCC^C
ApaLI 1815 GTGCAC G^TGCAC
AvaI 4363 CCCGAG C^[CT]CG[GA]G
BbeI 1182 GGCGCC GGCGC^C
BcgI 5872 CGAGGTTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 413 GTATCC GTATCC(6/5)
BcnI 1241 CCGGG CC^[GC]GG
BsaBI 565 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1182 GGCGCC G[GA]^CG[CT]C
BsaI 4596 GAGACC GGTCTC(1/5)
BsoBI 4363 CCCGAG C^[CT]CG[GA]G
BstAPI 981 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3861 GGTTACC G^GT[ACGT]ACC
BstPI 3861 GGTTACC G^GT[ACGT]ACC
BstUI 85 CGCG CG^CG
BstXI 3448 CCATGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 3446 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 3861 GGTTACC G^GT[ACGT]ACC
HaeII 1182 GGCGCC [GA]GCGC^[CT]
Hin1I 1182 GGCGCC G[GA]^CG[CT]C
HindIII 4606 AAGCTT A^AGCTT
KasI 1182 GGCGCC G^GCGCC
MamI 565 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 5890 CCGCTG C[AC]G^C[GT]G
MvnI 85 CGCG CG^CG
NaeI 1039 GCCGGC GCC^GGC
NarI 1182 GGCGCC GG^CGCC
NciI 1241 CCGGG CC^[GC]GG
NdeI 2205 CATATG CA^TATG
NgoMIV 1039 GCCGGC G^CCGGC
PflMI 4641 CCAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1499 ACCGGT A^CCGGT
SfoI 1182 GGCGCC GGC^GCC
StuI 5906 AGGCCT AGG^CCT
TaqI 2328 TCGA T^CGA
TthHB8I 2328 TCGA T^CGA
Van91I 4641 CCAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AflII 3506 CTTAAG C^TTAAG
5068 CTTAAG
AseI 2551 ATTAAT AT^TAAT
5601 ATTAAT
AsnI 2551 ATTAAT AT^TAAT
5601 ATTAAT
AvrII 2752 CCTAGG C^CTAGG
5318 CCTAGG
BbsI 3878 GAAGAC GAAGAC(2/6)
5313 GTCTTC
BbvCI 4354 CCTCAGC CCTCAGC(-5/-2)
4712 CCTCAGC
BclI 747 TGATCA T^GATCA
2395 TGATCA
BfrI 3506 CTTAAG C^TTAAG
5068 CTTAAG
BglII 373 AGATCT A^GATCT
2137 AGATCT
BlnI 2752 CCTAGG C^CTAGG
5318 CCTAGG
BpuAI 3878 GAAGAC GAAGAC(2/6)
5313 GTCTTC
BsaAI 3271 TACGTG [CT]AC^GT[GA]
4562 CACGTA
BsaWI 1255 ACCGGA [AT]^CCGG[AT]
1499 ACCGGT
BseRI 545 CTCCTC GAGGAG(10/8)
4147 CTCCTC
BsmBI 1457 CGTCTC CGTCTC(1/5)
4259 GAGACG
BsmI 2970 GAATGC GAATGC(1/-1)
2245 GCATTC
Bsp1407I 2319 TGTACA T^GTACA
2446 TGTACA
BspHI 744 TCATGA T^CATGA
1925 TCATGA
BspMI 1225 GCAGGT ACCTGC(4/8)
3265 GCAGGT
BsrBI 34 GAGCGG CCGCTC(-3/-3)
40 GAGCGG
BsrFI 1039 GCCGGC [GA]^CCGG[CT]
1499 ACCGGT
BsrGI 2319 TGTACA T^GTACA
2446 TGTACA
Bst1107I 3145 GTATAC GTA^TAC
4061 GTATAC
BstZ17I 3145 GTATAC GTA^TAC
4061 GTATAC
CfoI 1183 GCGC GCG^C
1337 GCGC
Cfr10I 1039 GCCGGC [GA]^CCGG[CT]
1499 ACCGGT
EcoNI 1290 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2295 CCTTAAAAAGG
FauI 36 GCGGG CCCGC(4/6)
42 GCGGG
FbaI 747 TGATCA T^GATCA
2395 TGATCA
HgaI 83 GACGC GACGC(5/10)
1756 GCGTC
HhaI 1183 GCGC GCG^C
1337 GCGC
HinP1I 1183 GCGC G^CGC
1337 GCGC
HincII 681 GTTGAC GT[CT]^[GA]AC
5968 GTTGAC
HindII 681 GTTGAC GT[CT]^[GA]AC
5968 GTTGAC
NcoI 950 CCATGG C^CATGG
5915 CCATGG
PshBI 2551 ATTAAT AT^TAAT
5601 ATTAAT
PstI 785 CTGCAG CTGCA^G
988 CTGCAG
RcaI 744 TCATGA T^CATGA
1925 TCATGA
SapI 1667 GCTCTTC GCTCTTC(1/4)
4785 GCTCTTC
SexAI 1295 ACCAGGT A^CC[AT]GGT
3088 ACCTGGT
SspBI 2319 TGTACA T^GTACA
2446 TGTACA
XcmI 399 CCAGATTCTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1431 CCAGAGCACACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AocI ApoI Asp700I
AspHI AvaII BamHI BanI BanII
BbvI BfaI BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EarI Eco81I EcoO109I EcoRI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PleI
PpuMI RsaI SacI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI