################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII Acc65I AccIII AcyI AflII AgeI Aor51HI AscI AseI AsnI Asp700I Asp718I AspI AviII BamHI BbeI BbrPI BfrI BsaHI BseAI BsiWI BsmBI BspDI BspEI BssHII Bst1107I BstBI BstZ17I ClaI CpoI Eco47III FspI Hin1I HindIII HpaI KasI KpnI KspI MroI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SphI Sse8387I SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AclI 4545 AACGTT AA^CGTT AhdI 2643 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1431 GTGCAC G^TGCAC ApaLI 1431 GTGCAC G^TGCAC AspEI 2643 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 4568 CTCGGG C^[CT]CG[GA]G BcgI 1205 GCAACCACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 746 GGATAC GTATCC(6/5) BclI 2509 TGATCA T^GATCA BglII 1820 AGATCT A^GATCT BsaAI 749 TACGTA [CT]AC^GT[GA] BsaBI 4201 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 1121 CGGCCG CG[GA][CT]^CG BsoBI 4568 CTCGGG C^[CT]CG[GA]G BspHI 285 TCATGA T^CATGA BsrDI 705 GCAATG GCAATG(2/0) EagI 1121 CGGCCG C^GGCCG Eam1105I 2643 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1121 CGGCCG C^GGCCG Eco52I 1121 CGGCCG C^GGCCG EcoRI 2351 GAATTC G^AATTC EcoRV 4117 GATATC GAT^ATC EcoT22I 5037 ATGCAT ATGCA^T FbaI 2509 TGATCA T^GATCA FseI 1118 GGCCGGCC GGCCGG^CC HgaI 2449 GCGTC GACGC(5/10) MamI 4201 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 204 CAATTG C^AATTG MluI 4990 ACGCGT A^CGCGT MunI 204 CAATTG C^AATTG NsiI 5037 ATGCAT ATGCA^T Psp1406I 4545 AACGTT AA^CGTT RcaI 285 TCATGA T^CATGA SexAI 3269 ACCAGGT A^CC[AT]GGT SnaBI 749 TACGTA TAC^GTA SpeI 2083 ACTAGT A^CTAGT SspI 2853 AATATT AAT^ATT XbaI 4110 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 1562 GTAGAC GT^[AC][GT]AC 4315 GTAGAC ApaI 3739 GGGCCC GGGCC^C 4501 GGGCCC AvrII 3690 CCTAGG C^CTAGG 3696 CCTAGG BbsI 16 GAAGAC GAAGAC(2/6) 3553 GAAGAC BglI 1104 GCCCCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4647 GCCTGAGGGGC BlnI 3690 CCTAGG C^CTAGG 3696 CCTAGG BpuAI 16 GAAGAC GAAGAC(2/6) 3553 GAAGAC BsaWI 2752 TCCGGT [AT]^CCGG[AT] 4197 ACCGGA BsmI 5035 GAATGC GAATGC(1/-1) 1779 GCATTC Bsp1407I 4032 TGTACA T^GTACA 4746 TGTACA BspLU11I 3426 ACATGT A^CATGT 3449 ACATGT BsrFI 1119 GCCGGC [GA]^CCGG[CT] 1545 GCCGGC BsrGI 4032 TGTACA T^GTACA 4746 TGTACA BssSI 3094 CACGAG CACGAG(-5/-1) 2988 CTCGTG BstYI 1820 AGATCT [GA]^GATC[CT] 3771 AGATCC Cfr10I 1119 GCCGGC [GA]^CCGG[CT] 1545 GCCGGC DraI 2770 TTTAAA TTT^AAA 5000 TTTAAA DraIII 1305 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG 4750 CACTCAGTG EcoNI 1149 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1668 CCTCGGTCAGG HaeII 552 GGCGCT [GA]GCGC^[CT] 573 GGCGCT MflI 1820 AGATCT [GA]^GATC[CT] 3771 AGATCC MslI 2876 CATTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 4574 CATCGACGTG NaeI 1119 GCCGGC GCC^GGC 1545 GCCGGC NcoI 1500 CCATGG C^CATGG 2227 CCATGG NgoMIV 1119 GCCGGC G^CCGGC 1545 GCCGGC PflMI 2009 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2137 CCACCACTTGG PpuMI 1960 GGGACCT [GA]G^G[AT]CC[CT] 3111 AGGACCT PvuII 4150 CAGCTG CAG^CTG 4443 CAGCTG SacI 2461 GAGCTC GAGCT^C 4171 GAGCTC SspBI 4032 TGTACA T^GTACA 4746 TGTACA Van91I 2009 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2137 CCACCACTTGG XhoII 1820 AGATCT [GA]^GATC[CT] 3771 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI Eco81I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI