##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspI AviII BamHI
BbeI BbrPI BfrI BsaHI BseAI
BsiWI BsmBI BspDI BspEI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
Eco47III FspI Hin1I HindIII HpaI
KasI KpnI KspI MroI NarI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SphI Sse8387I SwaI Tth111I XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AclI 4545 AACGTT AA^CGTT
AhdI 2643 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1431 GTGCAC G^TGCAC
ApaLI 1431 GTGCAC G^TGCAC
AspEI 2643 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 4568 CTCGGG C^[CT]CG[GA]G
BcgI 1205 GCAACCACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 746 GGATAC GTATCC(6/5)
BclI 2509 TGATCA T^GATCA
BglII 1820 AGATCT A^GATCT
BsaAI 749 TACGTA [CT]AC^GT[GA]
BsaBI 4201 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1121 CGGCCG CG[GA][CT]^CG
BsoBI 4568 CTCGGG C^[CT]CG[GA]G
BspHI 285 TCATGA T^CATGA
BsrDI 705 GCAATG GCAATG(2/0)
EagI 1121 CGGCCG C^GGCCG
Eam1105I 2643 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1121 CGGCCG C^GGCCG
Eco52I 1121 CGGCCG C^GGCCG
EcoRI 2351 GAATTC G^AATTC
EcoRV 4117 GATATC GAT^ATC
EcoT22I 5037 ATGCAT ATGCA^T
FbaI 2509 TGATCA T^GATCA
FseI 1118 GGCCGGCC GGCCGG^CC
HgaI 2449 GCGTC GACGC(5/10)
MamI 4201 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 204 CAATTG C^AATTG
MluI 4990 ACGCGT A^CGCGT
MunI 204 CAATTG C^AATTG
NsiI 5037 ATGCAT ATGCA^T
Psp1406I 4545 AACGTT AA^CGTT
RcaI 285 TCATGA T^CATGA
SexAI 3269 ACCAGGT A^CC[AT]GGT
SnaBI 749 TACGTA TAC^GTA
SpeI 2083 ACTAGT A^CTAGT
SspI 2853 AATATT AAT^ATT
XbaI 4110 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1562 GTAGAC GT^[AC][GT]AC
4315 GTAGAC
ApaI 3739 GGGCCC GGGCC^C
4501 GGGCCC
AvrII 3690 CCTAGG C^CTAGG
3696 CCTAGG
BbsI 16 GAAGAC GAAGAC(2/6)
3553 GAAGAC
BglI 1104 GCCCCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4647 GCCTGAGGGGC
BlnI 3690 CCTAGG C^CTAGG
3696 CCTAGG
BpuAI 16 GAAGAC GAAGAC(2/6)
3553 GAAGAC
BsaWI 2752 TCCGGT [AT]^CCGG[AT]
4197 ACCGGA
BsmI 5035 GAATGC GAATGC(1/-1)
1779 GCATTC
Bsp1407I 4032 TGTACA T^GTACA
4746 TGTACA
BspLU11I 3426 ACATGT A^CATGT
3449 ACATGT
BsrFI 1119 GCCGGC [GA]^CCGG[CT]
1545 GCCGGC
BsrGI 4032 TGTACA T^GTACA
4746 TGTACA
BssSI 3094 CACGAG CACGAG(-5/-1)
2988 CTCGTG
BstYI 1820 AGATCT [GA]^GATC[CT]
3771 AGATCC
Cfr10I 1119 GCCGGC [GA]^CCGG[CT]
1545 GCCGGC
DraI 2770 TTTAAA TTT^AAA
5000 TTTAAA
DraIII 1305 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
4750 CACTCAGTG
EcoNI 1149 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1668 CCTCGGTCAGG
HaeII 552 GGCGCT [GA]GCGC^[CT]
573 GGCGCT
MflI 1820 AGATCT [GA]^GATC[CT]
3771 AGATCC
MslI 2876 CATTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4574 CATCGACGTG
NaeI 1119 GCCGGC GCC^GGC
1545 GCCGGC
NcoI 1500 CCATGG C^CATGG
2227 CCATGG
NgoMIV 1119 GCCGGC G^CCGGC
1545 GCCGGC
PflMI 2009 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2137 CCACCACTTGG
PpuMI 1960 GGGACCT [GA]G^G[AT]CC[CT]
3111 AGGACCT
PvuII 4150 CAGCTG CAG^CTG
4443 CAGCTG
SacI 2461 GAGCTC GAGCT^C
4171 GAGCTC
SspBI 4032 TGTACA T^GTACA
4746 TGTACA
Van91I 2009 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2137 CCACCACTTGG
XhoII 1820 AGATCT [GA]^GATC[CT]
3771 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI Eco81I EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI