##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AclI AgeI AscI
AseI AsnI Asp718I AspI AviII
AvrII BbeI BclI BlnI BsaBI
BsiWI BsmBI Bsp1407I BspDI BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
DraIII EcoRI EcoRV FbaI FseI
FspI HindIII HpaI KasI KpnI
MamI MfeI MluI MunI NarI
NdeI NotI NruI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AflII 3643 CTTAAG C^TTAAG
AocI 3187 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3942 AGCGCT AGC^GCT
Asp700I 2731 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1530 GGATCC G^GATCC
BbrPI 3242 CACGTG CAC^GTG
BcgI 325 CGACTACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3643 CTTAAG C^TTAAG
BglI 960 GCCTGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1791 AGATCT A^GATCT
BlpI 3873 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3873 GCTGAGC GC^T[ACGT]AGC
BsrDI 255 GCAATG GCAATG(2/0)
BstEII 4053 GGTCACC G^GT[ACGT]ACC
BstPI 4053 GGTCACC G^GT[ACGT]ACC
Bsu36I 3187 CCTGAGG CC^T[ACGT]AGG
CelII 3873 GCTGAGC GC^T[ACGT]AGC
DrdI 896 GACGGGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3942 AGCGCT AGC^GCT
Eco81I 3187 CCTGAGG CC^T[ACGT]AGG
EcoO65I 4053 GGTCACC G^GT[ACGT]ACC
EcoT22I 4911 ATGCAT ATGCA^T
KspI 97 CCGCGG CCGC^GG
NaeI 1494 GCCGGC GCC^GGC
NgoMIV 1494 GCCGGC G^CCGGC
NheI 5157 GCTAGC G^CTAGC
NsiI 4911 ATGCAT ATGCA^T
PaeR7I 768 CTCGAG C^TCGAG
PmaCI 3242 CACGTG CAC^GTG
PmlI 3242 CACGTG CAC^GTG
PshAI 1283 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 97 CCGCGG CCGC^GG
SapI 1699 GCTCTTC GCTCTTC(1/4)
SexAI 342 ACCAGGT A^CC[AT]GGT
XbaI 4253 TCTAGA T^CTAGA
XhoI 768 CTCGAG C^TCGAG
XmnI 2731 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 181 GTCGAC GT^[AC][GT]AC
1225 GTCGAC
AccIII 1500 TCCGGA T^CCGGA
3602 TCCGGA
AcsI 5007 GAATTT [GA]^AATT[CT]
5619 AAATTT
AcyI 516 GACGCC G[GA]^CG[CT]C
2753 GACGCC
Alw44I 1901 GTGCAC G^TGCAC
4399 GTGCAC
ApaLI 1901 GTGCAC G^TGCAC
4399 GTGCAC
ApoI 5007 GAATTT [GA]^AATT[CT]
5619 AAATTT
BciVI 471 GTATCC GTATCC(6/5)
4969 GTATCC
BsaAI 758 TACGTG [CT]AC^GT[GA]
3242 CACGTG
BsaHI 516 GACGCC G[GA]^CG[CT]C
2753 GACGCC
BsaWI 1500 TCCGGA [AT]^CCGG[AT]
3602 TCCGGA
BseAI 1500 TCCGGA T^CCGGA
3602 TCCGGA
BsiEI 1492 CGGCCG CG[GA][CT]^CG
2422 CGGCCG
BsmI 4157 GAATGC GAATGC(1/-1)
4476 GAATGC
BspEI 1500 TCCGGA T^CCGGA
3602 TCCGGA
BspHI 2493 TCATGA T^CATGA
2640 TCATGA
BssHII 4595 GCGCGC G^CGCGC
4597 GCGCGC
DraI 3529 TTTAAA TTT^AAA
5598 TTTAAA
EagI 1492 CGGCCG C^GGCCG
2422 CGGCCG
EclXI 1492 CGGCCG C^GGCCG
2422 CGGCCG
Eco52I 1492 CGGCCG C^GGCCG
2422 CGGCCG
Hin1I 516 GACGCC G[GA]^CG[CT]C
2753 GACGCC
HincII 181 GTCGAC GT[CT]^[GA]AC
1225 GTCGAC
HindII 181 GTCGAC GT[CT]^[GA]AC
1225 GTCGAC
MroI 1500 TCCGGA T^CCGGA
3602 TCCGGA
RcaI 2493 TCATGA T^CATGA
2640 TCATGA
SalI 181 GTCGAC G^TCGAC
1225 GTCGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AfaI AflIII AhdI
AluI AlwI AlwNI ApaI AspEI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmaI SmlI SphI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI