##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AclI AgeI Alw44I
ApaI ApaLI AscI AviII AvrII
BamHI BclI BglI BglII BlnI
BlpI Bpu1102I BseAI BsiWI BspEI
BspHI BssHII BstEII BstPI CelII
CpoI DraIII DrdI EagI EclXI
Eco52I EcoO65I EcoRV FbaI FseI
FspI HpaI KspI MluI MroI
NaeI NdeI NgoMIV NheI NotI
NruI PacI PinAI PmeI Psp1406I
RcaI RsrII SacII SalI ScaI
SexAI SfiI SgrAI SmaI SpeI
SphI StuI SwaI XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 4408 GGTACC G^GTACC
AccI 2656 GTATAC GT^[AC][GT]AC
AhdI 2399 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4848 AGCGCT AGC^GCT
AseI 4546 ATTAAT AT^TAAT
AsnI 4546 ATTAAT AT^TAAT
Asp718I 4408 GGTACC G^GTACC
AspEI 2399 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 4700 CCTCAGC CCTCAGC(-5/-2)
BsaBI 3346 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3994 TCCGGT [AT]^CCGG[AT]
BsiEI 1195 CGATCG CG[GA][CT]^CG
BsmI 2620 GCATTC GAATGC(1/-1)
Bsp1407I 4236 TGTACA T^GTACA
BspDI 1211 ATCGAT AT^CGAT
BspLU11I 1147 ACATGT A^CATGT
BsrFI 336 ACCGGC [GA]^CCGG[CT]
BsrGI 4236 TGTACA T^GTACA
Bst1107I 2656 GTATAC GTA^TAC
BstBI 3573 TTCGAA TT^CGAA
BstYI 3906 AGATCC [GA]^GATC[CT]
BstZ17I 2656 GTATAC GTA^TAC
Cfr10I 336 ACCGGC [GA]^CCGG[CT]
ClaI 1211 ATCGAT AT^CGAT
Eam1105I 2399 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4848 AGCGCT AGC^GCT
EcoNI 490 CCTTGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1688 ATGCAT ATGCA^T
HincII 524 GTTGAC GT[CT]^[GA]AC
HindII 524 GTTGAC GT[CT]^[GA]AC
HindIII 2096 AAGCTT A^AGCTT
KpnI 4408 GGTACC GGTAC^C
MamI 3346 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3936 CAATTG C^AATTG
MflI 3906 AGATCC [GA]^GATC[CT]
MunI 3936 CAATTG C^AATTG
NcoI 3438 CCATGG C^CATGG
NsiI 1688 ATGCAT ATGCA^T
NspV 3573 TTCGAA TT^CGAA
PaeR7I 477 CTCGAG C^TCGAG
PflMI 4176 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 285 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4546 ATTAAT AT^TAAT
PvuI 1195 CGATCG CGAT^CG
SacI 1833 GAGCTC GAGCT^C
SbfI 3485 CCTGCAGG CCTGCA^GG
SfuI 3573 TTCGAA TT^CGAA
SnaBI 4441 TACGTA TAC^GTA
Sse8387I 3485 CCTGCAGG CCTGCA^GG
SspBI 4236 TGTACA T^GTACA
Van91I 4176 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1479 CCAAGGTTCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 477 CTCGAG C^TCGAG
XhoII 3906 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 466 CTTAAG C^TTAAG
2969 CTTAAG
AflIII 1147 ACATGT A^C[GA][CT]GT
1444 ACACGT
AocI 179 CCTGAGG CC^T[ACGT]AGG
4008 CCTCAGG
AspHI 270 GAGCAC G[AT]GC[AT]^C
1833 GAGCTC
AspI 887 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3212 GACAATGTC
AvaI 477 CTCGAG C^[CT]CG[GA]G
1986 CCCGAG
BbeI 2778 GGCGCC GGCGC^C
4729 GGCGCC
BbrPI 1445 CACGTG CAC^GTG
2393 CACGTG
BfaI 833 CTAG C^TAG
908 CTAG
BfrI 466 CTTAAG C^TTAAG
2969 CTTAAG
BsiHKAI 270 GAGCAC G[AT]GC[AT]^C
1833 GAGCTC
BsoBI 477 CTCGAG C^[CT]CG[GA]G
1986 CCCGAG
BssSI 1268 CACGAG CACGAG(-5/-1)
4819 CTCGTG
BstAPI 249 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
410 GCAAAACCTGC
BstXI 3462 CCAGTAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4844 CCAAAGCGCTGG
Bsu36I 179 CCTGAGG CC^T[ACGT]AGG
4008 CCTCAGG
Eco81I 179 CCTGAGG CC^T[ACGT]AGG
4008 CCTCAGG
EcoRI 3789 GAATTC G^AATTC
3855 GAATTC
KasI 2778 GGCGCC G^GCGCC
4729 GGCGCC
MaeI 833 CTAG C^TAG
908 CTAG
NarI 2778 GGCGCC GG^CGCC
4729 GGCGCC
PflFI 887 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3212 GACAATGTC
PmaCI 1445 CACGTG CAC^GTG
2393 CACGTG
PmlI 1445 CACGTG CAC^GTG
2393 CACGTG
PvuII 1916 CAGCTG CAG^CTG
3190 CAGCTG
SapI 2152 GAAGAGC GCTCTTC(1/4)
944 GCTCTTC
SfoI 2778 GGCGCC GGC^GCC
4729 GGCGCC
SspI 119 AATATT AAT^ATT
1670 AATATT
Tth111I 887 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3212 GACAATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AvaII BalI BanI BanII BbsI
BbvI BcgI BciVI BcnI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmnI