##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AccIII AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AspEI AspI
AviII AvrII BamHI BbrPI BcgI
BfrI BlnI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BssHII BstBI
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
FseI FspI HincII HindII HpaI
MluI MroI NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PvuI RsrII
SalI SapI SbfI SfuI SgrAI
Sse8387I SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 3307 GACGTC GACGT^C
Acc65I 714 GGTACC G^GTACC
AccI 4625 GTATAC GT^[AC][GT]AC
AclI 3874 AACGTT AA^CGTT
ApaI 781 GGGCCC GGGCC^C
Asp718I 714 GGTACC G^GTACC
AvaI 4883 CCCGGG C^[CT]CG[GA]G
BbeI 2518 GGCGCC GGCGC^C
BclI 825 TGATCA T^GATCA
BglII 4998 AGATCT A^GATCT
BlpI 3742 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3742 GCTGAGC GC^T[ACGT]AGC
BsaBI 2252 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 92 GAGACG CGTCTC(1/5)
BsoBI 4883 CCCGGG C^[CT]CG[GA]G
BssSI 3854 CTCGTG CACGAG(-5/-1)
Bst1107I 4625 GTATAC GTA^TAC
BstZ17I 4625 GTATAC GTA^TAC
CelII 3742 GCTGAGC GC^T[ACGT]AGC
EcoRV 3334 GATATC GAT^ATC
EcoT22I 1115 ATGCAT ATGCA^T
FbaI 825 TGATCA T^GATCA
KasI 2518 GGCGCC G^GCGCC
KpnI 714 GGTACC GGTAC^C
MamI 2252 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 2518 GGCGCC GG^CGCC
NsiI 1115 ATGCAT ATGCA^T
PshAI 4924 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3874 AACGTT AA^CGTT
SacI 5321 GAGCTC GAGCT^C
SexAI 3990 ACCTGGT A^CC[AT]GGT
SfiI 965 GGCCCAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2518 GGCGCC GGC^GCC
SmaI 4883 CCCGGG CCC^GGG
SnaBI 5225 TACGTA TAC^GTA
SpeI 4241 ACTAGT A^CTAGT
SphI 616 GCATGC GCATG^C
TaqI 935 TCGA T^CGA
TthHB8I 935 TCGA T^CGA
XmaCI 4883 CCCGGG C^CCGGG
XmaI 4883 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AocI 3667 CCTGAGG CC^T[ACGT]AGG
4658 CCTGAGG
AseI 1085 ATTAAT AT^TAAT
5376 ATTAAT
AsnI 1085 ATTAAT AT^TAAT
5376 ATTAAT
BbsI 2421 GTCTTC GAAGAC(2/6)
5183 GTCTTC
BbvCI 2055 GCTGAGG CCTCAGC(-5/-2)
2146 GCTGAGG
BpuAI 2421 GTCTTC GAAGAC(2/6)
5183 GTCTTC
Bsp1407I 2263 TGTACA T^GTACA
3034 TGTACA
BsrFI 183 GCCGGC [GA]^CCGG[CT]
2061 GCCGGC
BsrGI 2263 TGTACA T^GTACA
3034 TGTACA
BstAPI 336 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
893 GCAGGCCTTGC
BstEII 668 GGTCACC G^GT[ACGT]ACC
2592 GGTAACC
BstPI 668 GGTCACC G^GT[ACGT]ACC
2592 GGTAACC
Bsu36I 3667 CCTGAGG CC^T[ACGT]AGG
4658 CCTGAGG
Cfr10I 183 GCCGGC [GA]^CCGG[CT]
2061 GCCGGC
Eco81I 3667 CCTGAGG CC^T[ACGT]AGG
4658 CCTGAGG
EcoNI 651 CCTCCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4889 CCTGCAGAAGG
EcoO65I 668 GGTCACC G^GT[ACGT]ACC
2592 GGTAACC
HaeII 980 AGCGCC [GA]GCGC^[CT]
2518 GGCGCC
HindIII 1142 AAGCTT A^AGCTT
3463 AAGCTT
KspI 177 CCGCGG CCGC^GG
219 CCGCGG
MfeI 2946 CAATTG C^AATTG
5280 CAATTG
MunI 2946 CAATTG C^AATTG
5280 CAATTG
NaeI 183 GCCGGC GCC^GGC
2061 GCCGGC
NdeI 1183 CATATG CA^TATG
3486 CATATG
NgoMIV 183 GCCGGC G^CCGGC
2061 GCCGGC
PflMI 2031 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5237 CCAAGGAATGG
PshBI 1085 ATTAAT AT^TAAT
5376 ATTAAT
SacII 177 CCGCGG CCGC^GG
219 CCGCGG
ScaI 2614 AGTACT AGT^ACT
2741 AGTACT
SspBI 2263 TGTACA T^GTACA
3034 TGTACA
SspI 2665 AATATT AAT^ATT
3644 AATATT
Van91I 2031 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5237 CCAAGGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaII BalI BanI
BanII BbvI BciVI BcnI BfaI
BglI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BspLU11I BspMI BsrBI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII XmnI