##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
Acc65I AccIII AclI AgeI Alw44I
ApaLI AseI AsnI Asp718I AspI
AviII BbrPI BciVI BclI BsaAI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI Bst1107I BstBI BstZ17I Cfr10I
ClaI CpoI DraI DrdI EagI
EclXI Eco52I EcoRI EcoRV EcoT22I
FbaI FseI FspI KpnI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 21 enzymes) #
###################################
AatII 3768 GACGTC GACGT^C
AccI 824 GTCTAC GT^[AC][GT]AC
AflIII 5321 ACGTGT A^C[GA][CT]GT
Aor51HI 3061 AGCGCT AGC^GCT
AscI 76 GGCGCGCC GG^CGCGCC
Asp700I 2031 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 166 GCAACTGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 649 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BssHII 77 GCGCGC G^CGCGC
BstAPI 5239 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eco47III 3061 AGCGCT AGC^GCT
HpaI 2459 GTTAAC GTT^AAC
KspI 45 CCGCGG CCGC^GG
MamI 649 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 2246 GCTAGC G^CTAGC
PaeR7I 4673 CTCGAG C^TCGAG
PshAI 4273 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 45 CCGCGG CCGC^GG
ScaI 1574 AGTACT AGT^ACT
XhoI 4673 CTCGAG C^TCGAG
XmnI 2031 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflII 1616 CTTAAG C^TTAAG
4514 CTTAAG
BamHI 2082 GGATCC G^GATCC
4703 GGATCC
BbsI 2901 GAAGAC GAAGAC(2/6)
3765 GAAGAC
BfrI 1616 CTTAAG C^TTAAG
4514 CTTAAG
BglII 648 AGATCT A^GATCT
753 AGATCT
BpuAI 2901 GAAGAC GAAGAC(2/6)
3765 GAAGAC
BsmBI 3190 CGTCTC CGTCTC(1/5)
1037 GAGACG
BsrBI 3242 CCGCTC CCGCTC(-3/-3)
149 GAGCGG
EarI 1564 CTCTTC CTCTTC(1/4)
1686 CTCTTC
HgaI 555 GCGTC GACGC(5/10)
2796 GCGTC
HindIII 1964 AAGCTT A^AGCTT
4511 AAGCTT
Ksp632I 1564 CTCTTC CTCTTC(1/4)
1686 CTCTTC
MaeII 3769 ACGT A^CGT
5321 ACGT
NspI 1846 GCATGT [GA]CATG^[CT]
2748 ACATGC
SacI 778 GAGCTC GAGCT^C
1417 GAGCTC
SfiI 932 GGCCACAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2974 GGCCCTTTGGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI AlwNI AocI
ApaI ApoI AspEI AspHI AvaI
AvaII AvrII BalI BanI BanII
BbeI BbvCI BbvI BcnI BfaI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BssSI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI Eam1105I Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI