##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AspI AvaI AviII AvrII
BbeI BbrPI BbvCI BcgI BglI
BlnI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiWI BsmBI
BsoBI BspEI BsrBI BssSI BstEII
BstPI Bsu36I CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FseI
FspI Hin1I HindIII KasI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SphI Sse8387I Tth111I XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 1154 GTATAC GT^[AC][GT]AC
AclI 4842 AACGTT AA^CGTT
AflII 4928 CTTAAG C^TTAAG
AhdI 470 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 277 GGGCCC GGGCC^C
AseI 3186 ATTAAT AT^TAAT
AsnI 3186 ATTAAT AT^TAAT
AspEI 470 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1796 TGGCCA TGG^CCA
BanI 344 GGCACC G^G[CT][GA]CC
BfrI 4928 CTTAAG C^TTAAG
BlpI 5006 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 5006 GCTGAGC GC^T[ACGT]AGC
BsiEI 318 CGGTCG CG[GA][CT]^CG
BspLU11I 4452 ACATGT A^CATGT
BsrFI 184 ACCGGC [GA]^CCGG[CT]
Bst1107I 1154 GTATAC GTA^TAC
BstAPI 345 GCACCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1913 TTCGAA TT^CGAA
BstZ17I 1154 GTATAC GTA^TAC
CelII 5006 GCTGAGC GC^T[ACGT]AGC
Cfr10I 184 ACCGGC [GA]^CCGG[CT]
EaeI 1796 TGGCCA [CT]^GGCC[GA]
Eam1105I 470 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HgaI 36 GACGC GACGC(5/10)
HpaI 3629 GTTAAC GTT^AAC
MluNI 1796 TGGCCA TGG^CCA
MscI 1796 TGGCCA TGG^CCA
MspA1I 423 CAGCTG C[AC]G^C[GT]G
NcoI 5401 CCATGG C^CATGG
NheI 1855 GCTAGC G^CTAGC
NspV 1913 TTCGAA TT^CGAA
PflMI 2964 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 503 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1504 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3186 ATTAAT AT^TAAT
Psp1406I 4842 AACGTT AA^CGTT
PvuII 423 CAGCTG CAG^CTG
SapI 1074 GCTCTTC GCTCTTC(1/4)
SfuI 1913 TTCGAA TT^CGAA
SpeI 3291 ACTAGT A^CTAGT
SwaI 4914 ATTTAAAT ATTT^AAAT
Van91I 2964 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvaII 504 GGACC G^G[AT]CC
1026 GGTCC
BamHI 1785 GGATCC G^GATCC
4200 GGATCC
BciVI 950 GGATAC GTATCC(6/5)
3196 GGATAC
BclI 1849 TGATCA T^GATCA
2691 TGATCA
BcnI 316 CCCGG CC^[GC]GG
603 CCGGG
BglII 1014 AGATCT A^GATCT
1242 AGATCT
BspDI 3015 ATCGAT AT^CGAT
3513 ATCGAT
BspMI 1023 GCAGGT ACCTGC(4/8)
4425 GCAGGT
BssHII 553 GCGCGC G^CGCGC
758 GCGCGC
BstXI 1695 CCAGGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2795 CCACTGCCCTGG
ClaI 3015 ATCGAT AT^CGAT
3513 ATCGAT
DsaI 4170 CCGTGG C^C[GA][CT]GG
5401 CCATGG
EarI 924 CTCTTC CTCTTC(1/4)
1075 CTCTTC
EcoT14I 3871 CCAAGG C^C[AT][AT]GG
5401 CCATGG
EcoT22I 2552 ATGCAT ATGCA^T
3487 ATGCAT
FbaI 1849 TGATCA T^GATCA
2691 TGATCA
HaeII 51 AGCGCC [GA]GCGC^[CT]
1233 GGCGCT
Ksp632I 924 CTCTTC CTCTTC(1/4)
1075 CTCTTC
NciI 316 CCCGG CC^[GC]GG
603 CCGGG
NsiI 2552 ATGCAT ATGCA^T
3487 ATGCAT
ScaI 2401 AGTACT AGT^ACT
3859 AGTACT
StuI 2049 AGGCCT AGG^CCT
3806 AGGCCT
StyI 3871 CCAAGG C^C[AT][AT]GG
5401 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspHI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PleI PstI RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII XmnI