##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AccIII AflII AgeI AhdI Aor51HI
ApaI AscI AspEI AviII BbvCI
BfrI BglI BsaWI BseAI BsiWI
BspDI BspEI BsrBI BssHII BstAPI
ClaI CpoI Eam1105I Eco47III EcoNI
EcoRI EcoT22I FspI HgaI KspI
MfeI MluI MroI MunI NdeI
NotI NruI NsiI PaeR7I PinAI
PmeI PvuI RsrII SacI SacII
SalI SapI SbfI SfiI SgrAI
SnaBI Sse8387I StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AatII 2263 GACGTC GACGT^C
Acc65I 1175 GGTACC G^GTACC
AclI 2709 AACGTT AA^CGTT
Alw44I 69 GTGCAC G^TGCAC
AocI 1570 CCTTAGG CC^T[ACGT]AGG
ApaLI 69 GTGCAC G^TGCAC
Asp718I 1175 GGTACC G^GTACC
AspHI 69 GTGCAC G[AT]GC[AT]^C
AspI 905 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1617 CCTAGG C^CTAGG
BamHI 3862 GGATCC G^GATCC
BbrPI 3640 CACGTG CAC^GTG
BcgI 883 CGATTACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1617 CCTAGG C^CTAGG
BsiEI 32 CGGCCG CG[GA][CT]^CG
BsiHKAI 69 GTGCAC G[AT]GC[AT]^C
BsmBI 921 GAGACG CGTCTC(1/5)
BsrFI 34 GCCGGC [GA]^CCGG[CT]
BssSI 3679 CTCGTG CACGAG(-5/-1)
Bst1107I 2013 GTATAC GTA^TAC
BstBI 2401 TTCGAA TT^CGAA
BstEII 2479 GGTCACC G^GT[ACGT]ACC
BstPI 2479 GGTCACC G^GT[ACGT]ACC
BstZ17I 2013 GTATAC GTA^TAC
Bsu36I 1570 CCTTAGG CC^T[ACGT]AGG
Cfr10I 34 GCCGGC [GA]^CCGG[CT]
EagI 32 CGGCCG C^GGCCG
EarI 556 GAAGAG CTCTTC(1/4)
EclXI 32 CGGCCG C^GGCCG
Eco52I 32 CGGCCG C^GGCCG
Eco81I 1570 CCTTAGG CC^T[ACGT]AGG
EcoO65I 2479 GGTCACC G^GT[ACGT]ACC
EcoRV 2643 GATATC GAT^ATC
FseI 33 GGCCGGCC GGCCGG^CC
HpaI 5593 GTTAAC GTT^AAC
KpnI 1175 GGTACC GGTAC^C
Ksp632I 556 GAAGAG CTCTTC(1/4)
NaeI 34 GCCGGC GCC^GGC
NcoI 4072 CCATGG C^CATGG
NgoMIV 34 GCCGGC G^CCGGC
NheI 2429 GCTAGC G^CTAGC
NspV 2401 TTCGAA TT^CGAA
PacI 1032 TTAATTAA TTAAT^TAA
PflFI 905 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3483 CCATAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3640 CACGTG CAC^GTG
PmlI 3640 CACGTG CAC^GTG
PpuMI 863 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1689 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2709 AACGTT AA^CGTT
PstI 2469 CTGCAG CTGCA^G
PvuII 1841 CAGCTG CAG^CTG
ScaI 2618 AGTACT AGT^ACT
SexAI 1260 ACCTGGT A^CC[AT]GGT
SfuI 2401 TTCGAA TT^CGAA
SmaI 211 CCCGGG CCC^GGG
SpeI 2716 ACTAGT A^CTAGT
Tth111I 905 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3483 CCATAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 211 CCCGGG C^CCGGG
XmaI 211 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1331 GTAGAC GT^[AC][GT]AC
2013 GTATAC
AflIII 2009 ACATGT A^C[GA][CT]GT
3929 ACATGT
AvaI 22 CCCGAG C^[CT]CG[GA]G
211 CCCGGG
BalI 1125 TGGCCA TGG^CCA
2935 TGGCCA
BanII 19 GAGCCC G[GA]GC[CT]^C
151 GGGCTC
BglII 3402 AGATCT A^GATCT
4227 AGATCT
BlpI 4274 GCTGAGC GC^T[ACGT]AGC
5331 GCTGAGC
Bpu1102I 4274 GCTGAGC GC^T[ACGT]AGC
5331 GCTGAGC
BsaAI 3348 CACGTA [CT]AC^GT[GA]
3640 CACGTG
BsaBI 1989 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4222 GATGCAGATC
BsmFI 1356 GTCCC GGGAC(10/14)
5300 GTCCC
BsoBI 22 CCCGAG C^[CT]CG[GA]G
211 CCCGGG
Bsp1407I 2984 TGTACA T^GTACA
5415 TGTACA
BspHI 225 TCATGA T^CATGA
1473 TCATGA
BspLU11I 2009 ACATGT A^CATGT
3929 ACATGT
BspMI 2542 ACCTGC ACCTGC(4/8)
3062 ACCTGC
BsrGI 2984 TGTACA T^GTACA
5415 TGTACA
BstXI 793 CCAAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5112 CCATAAACTTGG
CelII 4274 GCTGAGC GC^T[ACGT]AGC
5331 GCTGAGC
DraII 50 AGGCCCC [GA]G^G[ACGT]CC[CT]
863 AGGACCT
DraIII 716 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
1259 CACCTGGTG
DrdI 817 GACCAGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
929 GACTACATCGTC
EcoO109I 50 AGGCCCC [GA]G^G[ACGT]CC[CT]
863 AGGACCT
MamI 1989 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4222 GATGCAGATC
MluNI 1125 TGGCCA TGG^CCA
2935 TGGCCA
MscI 1125 TGGCCA TGG^CCA
2935 TGGCCA
RcaI 225 TCATGA T^CATGA
1473 TCATGA
SphI 3469 GCATGC GCATG^C
3758 GCATGC
SspBI 2984 TGTACA T^GTACA
5415 TGTACA
TaqI 2402 TCGA T^CGA
4106 TCGA
TthHB8I 2402 TCGA T^CGA
4106 TCGA
XbaI 1579 TCTAGA T^CTAGA
3956 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AvaII BanI BbeI
BbsI BbvI BciVI BclI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EaeI EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI KasI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PleI
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII XmnI