##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI Asp718I
AspI AvaI AviII AvrII BalI
BanI BbeI BfrI BlnI BlpI
Bpu1102I BsaBI BsiEI BsiWI BsmBI
BsoBI BspDI BssHII Bst1107I BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EaeI EagI EclXI Eco47III
Eco52I EcoNI EcoT22I FseI FspI
HaeII KasI KpnI KspI MamI
MluI MluNI MscI NaeI NarI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SphI Sse8387I SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 2408 GTAGAC GT^[AC][GT]AC
AccII 3729 CGCG CG^CG
AccIII 3686 TCCGGA T^CCGGA
AcyI 1302 GGCGTC G[GA]^CG[CT]C
AlwNI 4383 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 822 GGGCCC GGGCC^C
BamHI 4112 GGATCC G^GATCC
BbrPI 2714 CACGTG CAC^GTG
BbvCI 461 CCTCAGC CCTCAGC(-5/-2)
BcgI 515 CGATGGTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3676 TGATCA T^GATCA
BglI 3606 GCCTGTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4772 AGATCT A^GATCT
BsaHI 1302 GGCGTC G[GA]^CG[CT]C
BseAI 3686 TCCGGA T^CCGGA
BspEI 3686 TCCGGA T^CCGGA
BspHI 4003 TCATGA T^CATGA
BsrBI 181 CCGCTC CCGCTC(-3/-3)
BssSI 4760 CTCGTG CACGAG(-5/-1)
BstEII 364 GGTGACC G^GT[ACGT]ACC
BstPI 364 GGTGACC G^GT[ACGT]ACC
BstUI 3729 CGCG CG^CG
BstXI 306 CCAACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoO65I 364 GGTGACC G^GT[ACGT]ACC
FauI 180 CCCGC CCCGC(4/6)
FbaI 3676 TGATCA T^GATCA
Hin1I 1302 GGCGTC G[GA]^CG[CT]C
HincII 835 GTTAAC GT[CT]^[GA]AC
HindII 835 GTTAAC GT[CT]^[GA]AC
HpaI 835 GTTAAC GTT^AAC
MroI 3686 TCCGGA T^CCGGA
MvnI 3729 CGCG CG^CG
NdeI 2928 CATATG CA^TATG
PmaCI 2714 CACGTG CAC^GTG
PmlI 2714 CACGTG CAC^GTG
RcaI 4003 TCATGA T^CATGA
SacI 2454 GAGCTC GAGCT^C
SpeI 254 ACTAGT A^CTAGT
StuI 4077 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 2020 ACATGT A^C[GA][CT]GT
4334 ACATGT
AhdI 1562 GACTTAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2234 GACGTGAAGTC
AocI 33 CCTCAGG CC^T[ACGT]AGG
3376 CCTGAGG
AspEI 1562 GACTTAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2234 GACGTGAAGTC
BciVI 246 GTATCC GTATCC(6/5)
4849 GTATCC
BcnI 1402 CCGGG CC^[GC]GG
2430 CCCGG
BsaAI 2645 CACGTA [CT]AC^GT[GA]
2714 CACGTG
BsaI 4544 GAGACC GGTCTC(1/5)
2119 GGTCTC
Bsp1407I 2861 TGTACA T^GTACA
4612 TGTACA
BspLU11I 2020 ACATGT A^CATGT
4334 ACATGT
BspMI 2398 ACCTGC ACCTGC(4/8)
4547 ACCTGC
BsrFI 1311 GCCGGT [GA]^CCGG[CT]
2845 ACCGGC
BsrGI 2861 TGTACA T^GTACA
4612 TGTACA
BstAPI 2396 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2606 GCAACACTTGC
Bsu36I 33 CCTCAGG CC^T[ACGT]AGG
3376 CCTGAGG
Cfr10I 1311 GCCGGT [GA]^CCGG[CT]
2845 ACCGGC
Eam1105I 1562 GACTTAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2234 GACGTGAAGTC
Eco81I 33 CCTCAGG CC^T[ACGT]AGG
3376 CCTGAGG
HindIII 639 AAGCTT A^AGCTT
1598 AAGCTT
MfeI 903 CAATTG C^AATTG
3803 CAATTG
MunI 903 CAATTG C^AATTG
3803 CAATTG
NciI 1402 CCGGG CC^[GC]GG
2430 CCCGG
PpuMI 49 GGGTCCC [GA]G^G[AT]CC[CT]
952 GGGACCT
PvuII 4065 CAGCTG CAG^CTG
4626 CAGCTG
SmlI 1824 CTCAAG C^T[CT][GA]AG
2903 CTTGAG
SspBI 2861 TGTACA T^GTACA
4612 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I AspHI
AvaII BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrDI
BsrI BssKI BstBI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRI EcoRII EcoRV EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI NspV
PleI PshBI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfuI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI