################################## # NO CUT ENZYMES ( 46 enzymes) # ################################## AccI AclI AgeI AscI AseI AsnI BbeI BsaBI BspDI BsrDI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DrdI EcoRV FseI HpaI KasI MamI MluI NarI NdeI NheI NotI NruI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RsrII SalI SfiI SfoI SfuI SgrAI SnaBI SpeI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 2417 TCCGGA T^CCGGA AflII 4793 CTTAAG C^TTAAG Alw44I 4156 GTGCAC G^TGCAC ApaLI 4156 GTGCAC G^TGCAC AviII 4338 TGCGCA TGC^GCA AvrII 4330 CCTAGG C^CTAGG BamHI 2380 GGATCC G^GATCC BfrI 4793 CTTAAG C^TTAAG BglI 5003 GCCAACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3005 AGATCT A^GATCT BlnI 4330 CCTAGG C^CTAGG BsaWI 2417 TCCGGA [AT]^CCGG[AT] BseAI 2417 TCCGGA T^CCGGA BsiEI 496 CGGCCG CG[GA][CT]^CG Bsp1407I 2177 TGTACA T^GTACA BspEI 2417 TCCGGA T^CCGGA BspHI 3197 TCATGA T^CATGA BsrGI 2177 TGTACA T^GTACA BssSI 759 CACGAG CACGAG(-5/-1) DraI 3235 TTTAAA TTT^AAA EagI 496 CGGCCG C^GGCCG EclXI 496 CGGCCG C^GGCCG Eco52I 496 CGGCCG C^GGCCG EcoT22I 109 ATGCAT ATGCA^T FspI 4338 TGCGCA TGC^GCA HincII 4144 GTCAAC GT[CT]^[GA]AC HindII 4144 GTCAAC GT[CT]^[GA]AC HindIII 2623 AAGCTT A^AGCTT KspI 946 CCGCGG CCGC^GG MfeI 3797 CAATTG C^AATTG MroI 2417 TCCGGA T^CCGGA MunI 3797 CAATTG C^AATTG NsiI 109 ATGCAT ATGCA^T PshAI 2515 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 3197 TCATGA T^CATGA SacII 946 CCGCGG CCGC^GG SapI 1885 GAAGAGC GCTCTTC(1/4) SbfI 972 CCTGCAGG CCTGCA^GG ScaI 3317 AGTACT AGT^ACT Sse8387I 972 CCTGCAGG CCTGCA^GG SspBI 2177 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AatII 1963 GACGTC GACGT^C 4222 GACGTC Acc65I 62 GGTACC G^GTACC 1484 GGTACC AhdI 2101 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4351 GACATGGGGTC Aor51HI 1158 AGCGCT AGC^GCT 2112 AGCGCT Asp718I 62 GGTACC G^GTACC 1484 GGTACC AspEI 2101 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4351 GACATGGGGTC BbrPI 1735 CACGTG CAC^GTG 2048 CACGTG BbvCI 23 CCTCAGC CCTCAGC(-5/-2) 5091 GCTGAGG BciVI 3882 GGATAC GTATCC(6/5) 3713 GTATCC BlpI 3333 GCTCAGC GC^T[ACGT]AGC 4723 GCTGAGC Bpu1102I 3333 GCTCAGC GC^T[ACGT]AGC 4723 GCTGAGC BsaI 1696 GAGACC GGTCTC(1/5) 4991 GAGACC BsiWI 2330 CGTACG C^GTACG 2450 CGTACG BspMI 470 ACCTGC ACCTGC(4/8) 2556 GCAGGT CelII 3333 GCTCAGC GC^T[ACGT]AGC 4723 GCTGAGC Eam1105I 2101 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4351 GACATGGGGTC Eco47III 1158 AGCGCT AGC^GCT 2112 AGCGCT EcoRI 1016 GAATTC G^AATTC 3134 GAATTC KpnI 62 GGTACC GGTAC^C 1484 GGTACC PaeR7I 1801 CTCGAG C^TCGAG 3475 CTCGAG PmaCI 1735 CACGTG CAC^GTG 2048 CACGTG PmlI 1735 CACGTG CAC^GTG 2048 CACGTG SexAI 3416 ACCAGGT A^CC[AT]GGT 3886 ACCAGGT XhoI 1801 CTCGAG C^TCGAG 3475 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 147 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI Asp700I AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvI BcgI BclI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspLU11I BsrBI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI