##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AccI AclI AgeI AscI AseI
AsnI BbeI BsaBI BspDI BsrDI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DrdI EcoRV FseI HpaI
KasI MamI MluI NarI NdeI
NheI NotI NruI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI SfiI SfoI SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 2417 TCCGGA T^CCGGA
AflII 4793 CTTAAG C^TTAAG
Alw44I 4156 GTGCAC G^TGCAC
ApaLI 4156 GTGCAC G^TGCAC
AviII 4338 TGCGCA TGC^GCA
AvrII 4330 CCTAGG C^CTAGG
BamHI 2380 GGATCC G^GATCC
BfrI 4793 CTTAAG C^TTAAG
BglI 5003 GCCAACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3005 AGATCT A^GATCT
BlnI 4330 CCTAGG C^CTAGG
BsaWI 2417 TCCGGA [AT]^CCGG[AT]
BseAI 2417 TCCGGA T^CCGGA
BsiEI 496 CGGCCG CG[GA][CT]^CG
Bsp1407I 2177 TGTACA T^GTACA
BspEI 2417 TCCGGA T^CCGGA
BspHI 3197 TCATGA T^CATGA
BsrGI 2177 TGTACA T^GTACA
BssSI 759 CACGAG CACGAG(-5/-1)
DraI 3235 TTTAAA TTT^AAA
EagI 496 CGGCCG C^GGCCG
EclXI 496 CGGCCG C^GGCCG
Eco52I 496 CGGCCG C^GGCCG
EcoT22I 109 ATGCAT ATGCA^T
FspI 4338 TGCGCA TGC^GCA
HincII 4144 GTCAAC GT[CT]^[GA]AC
HindII 4144 GTCAAC GT[CT]^[GA]AC
HindIII 2623 AAGCTT A^AGCTT
KspI 946 CCGCGG CCGC^GG
MfeI 3797 CAATTG C^AATTG
MroI 2417 TCCGGA T^CCGGA
MunI 3797 CAATTG C^AATTG
NsiI 109 ATGCAT ATGCA^T
PshAI 2515 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3197 TCATGA T^CATGA
SacII 946 CCGCGG CCGC^GG
SapI 1885 GAAGAGC GCTCTTC(1/4)
SbfI 972 CCTGCAGG CCTGCA^GG
ScaI 3317 AGTACT AGT^ACT
Sse8387I 972 CCTGCAGG CCTGCA^GG
SspBI 2177 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 1963 GACGTC GACGT^C
4222 GACGTC
Acc65I 62 GGTACC G^GTACC
1484 GGTACC
AhdI 2101 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4351 GACATGGGGTC
Aor51HI 1158 AGCGCT AGC^GCT
2112 AGCGCT
Asp718I 62 GGTACC G^GTACC
1484 GGTACC
AspEI 2101 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4351 GACATGGGGTC
BbrPI 1735 CACGTG CAC^GTG
2048 CACGTG
BbvCI 23 CCTCAGC CCTCAGC(-5/-2)
5091 GCTGAGG
BciVI 3882 GGATAC GTATCC(6/5)
3713 GTATCC
BlpI 3333 GCTCAGC GC^T[ACGT]AGC
4723 GCTGAGC
Bpu1102I 3333 GCTCAGC GC^T[ACGT]AGC
4723 GCTGAGC
BsaI 1696 GAGACC GGTCTC(1/5)
4991 GAGACC
BsiWI 2330 CGTACG C^GTACG
2450 CGTACG
BspMI 470 ACCTGC ACCTGC(4/8)
2556 GCAGGT
CelII 3333 GCTCAGC GC^T[ACGT]AGC
4723 GCTGAGC
Eam1105I 2101 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4351 GACATGGGGTC
Eco47III 1158 AGCGCT AGC^GCT
2112 AGCGCT
EcoRI 1016 GAATTC G^AATTC
3134 GAATTC
KpnI 62 GGTACC GGTAC^C
1484 GGTACC
PaeR7I 1801 CTCGAG C^TCGAG
3475 CTCGAG
PmaCI 1735 CACGTG CAC^GTG
2048 CACGTG
PmlI 1735 CACGTG CAC^GTG
2048 CACGTG
SexAI 3416 ACCAGGT A^CC[AT]GGT
3886 ACCAGGT
XhoI 1801 CTCGAG C^TCGAG
3475 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI Asp700I AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcgI BclI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspLU11I BsrBI
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI