##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AscI Asp700I Asp718I AspI AviII
AvrII BbrPI BciVI BclI BfrI
BlnI BsaAI BseAI BsiWI BspDI
BspEI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI EcoRI EcoRV FbaI
FseI FspI KpnI MluI MroI
NdeI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PvuI RsrII
SapI SbfI SexAI SfiI SfuI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AclI 1476 AACGTT AA^CGTT
AhdI 2267 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3425 GTGCAC G^TGCAC
AocI 4650 CCTTAGG CC^T[ACGT]AGG
ApaI 3 GGGCCC GGGCC^C
ApaLI 3425 GTGCAC G^TGCAC
AspEI 2267 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2960 TGGCCA TGG^CCA
BbeI 1781 GGCGCC GGCGC^C
BglI 1977 GCCCAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3605 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3605 GCTTAGC GC^T[ACGT]AGC
BsaWI 3242 ACCGGA [AT]^CCGG[AT]
BsiEI 42 CGGCCG CG[GA][CT]^CG
BspLU11I 4799 ACATGT A^CATGT
BspMI 1967 ACCTGC ACCTGC(4/8)
BstEII 4564 GGTAACC G^GT[ACGT]ACC
BstPI 4564 GGTAACC G^GT[ACGT]ACC
Bsu36I 4650 CCTTAGG CC^T[ACGT]AGG
CelII 3605 GCTTAGC GC^T[ACGT]AGC
DrdI 2985 GACCAGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 42 CGGCCG C^GGCCG
Eam1105I 2267 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 42 CGGCCG C^GGCCG
Eco52I 42 CGGCCG C^GGCCG
Eco81I 4650 CCTTAGG CC^T[ACGT]AGG
EcoNI 188 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4564 GGTAACC G^GT[ACGT]ACC
HindIII 3549 AAGCTT A^AGCTT
HpaI 4461 GTTAAC GTT^AAC
KasI 1781 GGCGCC G^GCGCC
MluNI 2960 TGGCCA TGG^CCA
MscI 2960 TGGCCA TGG^CCA
NarI 1781 GGCGCC GG^CGCC
NheI 2191 GCTAGC G^CTAGC
NspI 4799 ACATGT [GA]CATG^[CT]
Psp1406I 1476 AACGTT AA^CGTT
SalI 3178 GTCGAC G^TCGAC
ScaI 5143 AGTACT AGT^ACT
SfoI 1781 GGCGCC GGC^GCC
SgrAI 129 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 7 CCCGGG CCC^GGG
SmlI 312 CTCAAG C^T[CT][GA]AG
SspI 3943 AATATT AAT^ATT
StuI 4752 AGGCCT AGG^CCT
XcmI 2910 CCAGCGTGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 7 CCCGGG C^CCGGG
XmaI 7 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 1919 ACGTGT A^C[GA][CT]GT
4799 ACATGT
Aor51HI 1248 AGCGCT AGC^GCT
1342 AGCGCT
AseI 4161 ATTAAT AT^TAAT
5044 ATTAAT
AsnI 4161 ATTAAT AT^TAAT
5044 ATTAAT
BamHI 2234 GGATCC G^GATCC
3329 GGATCC
BanI 731 GGTGCC G^G[CT][GA]CC
1781 GGCGCC
BbvCI 1953 CCTCAGC CCTCAGC(-5/-2)
2952 GCTGAGG
BcgI 1905 CGAGAGTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3417 CGACTTCAGTGC
BglII 851 AGATCT A^GATCT
1018 AGATCT
BsaBI 1628 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2919 GATTGGCATC
BsmBI 1298 GAGACG CGTCTC(1/5)
2653 GAGACG
BsmI 527 GAATGC GAATGC(1/-1)
1900 GCATTC
Bsp1407I 5012 TGTACA T^GTACA
5083 TGTACA
BsrDI 3308 GCAATG GCAATG(2/0)
3704 GCAATG
BsrGI 5012 TGTACA T^GTACA
5083 TGTACA
BstXI 606 CCAGCGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3125 CCAGGATGCTGG
DraII 3 GGGCCCC [GA]G^G[ACGT]CC[CT]
181 AGGCCCC
DraIII 1137 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
4697 CACACAGTG
Eco47III 1248 AGCGCT AGC^GCT
1342 AGCGCT
EcoO109I 3 GGGCCCC [GA]G^G[ACGT]CC[CT]
181 AGGCCCC
EcoT22I 1208 ATGCAT ATGCA^T
4141 ATGCAT
MamI 1628 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2919 GATTGGCATC
MfeI 3338 CAATTG C^AATTG
4297 CAATTG
MunI 3338 CAATTG C^AATTG
4297 CAATTG
NsiI 1208 ATGCAT ATGCA^T
4141 ATGCAT
PflMI 1330 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4930 CCATGGCGTGG
PshBI 4161 ATTAAT AT^TAAT
5044 ATTAAT
PstI 1430 CTGCAG CTGCA^G
4358 CTGCAG
SspBI 5012 TGTACA T^GTACA
5083 TGTACA
Tsp45I 980 GTGAC ^GT[GC]AC
3649 GTGAC
Van91I 1330 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4930 CCATGGCGTGG
XbaI 2127 TCTAGA T^CTAGA
3010 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DsaI EaeI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PleI PvuII RcaI RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII