##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Aor51HI AscI Asp700I AspEI AspI
AviII BalI BbeI BbrPI BcgI
BlpI Bpu1102I BsaAI BsaI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraIII
Eam1105I Eco47III EcoNI EcoO65I EcoRV
FseI FspI HaeII HhaI HinP1I
KasI MluI MluNI MroI MscI
NaeI NarI NcoI NgoMIV NheI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmlI Psp1406I PvuI
RsrII SacI SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I Tth111I XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 1828 GGTACC G^GTACC
AccII 270 CGCG CG^CG
AcyI 2695 GACGCC G[GA]^CG[CT]C
AflII 576 CTTAAG C^TTAAG
Alw44I 1796 GTGCAC G^TGCAC
AlwNI 3731 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1796 GTGCAC G^TGCAC
Asp718I 1828 GGTACC G^GTACC
AvrII 142 CCTAGG C^CTAGG
BamHI 1095 GGATCC G^GATCC
BanI 1828 GGTACC G^G[CT][GA]CC
BbvCI 3124 CCTCAGC CCTCAGC(-5/-2)
BciVI 4591 GGATAC GTATCC(6/5)
BfrI 576 CTTAAG C^TTAAG
BglII 4039 AGATCT A^GATCT
BlnI 142 CCTAGG C^CTAGG
BsaHI 2695 GACGCC G[GA]^CG[CT]C
BsgI 5468 GTGCAG GTGCAG(16/14)
BsrBI 477 GAGCGG CCGCTC(-3/-3)
BstUI 270 CGCG CG^CG
DrdI 3183 GACTGATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 74 GCGGG CCCGC(4/6)
Hin1I 2695 GACGCC G[GA]^CG[CT]C
HindIII 2712 AAGCTT A^AGCTT
KpnI 1828 GGTACC GGTAC^C
KspI 269 CCGCGG CCGC^GG
MfeI 1833 CAATTG C^AATTG
MunI 1833 CAATTG C^AATTG
MvnI 270 CGCG CG^CG
NotI 265 GCGGCCGC GC^GGCCGC
PflMI 4198 CCACGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 4905 GTTTAAAC GTTT^AAAC
PstI 3140 CTGCAG CTGCA^G
SacII 269 CCGCGG CCGC^GG
StuI 2704 AGGCCT AGG^CCT
SwaI 5342 ATTTAAAT ATTT^AAAT
Van91I 4198 CCACGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AocI 296 CCTGAGG CC^T[ACGT]AGG
1052 CCTCAGG
ApaI 124 GGGCCC GGGCC^C
243 GGGCCC
AspHI 614 GTGCTC G[AT]GC[AT]^C
1796 GTGCAC
BclI 2564 TGATCA T^GATCA
5281 TGATCA
BsaBI 2797 GATAGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2959 GATATTAATC
BsiHKAI 614 GTGCTC G[AT]GC[AT]^C
1796 GTGCAC
BsmI 1939 GAATGC GAATGC(1/-1)
2142 GCATTC
Bsp1407I 1552 TGTACA T^GTACA
4933 TGTACA
BspHI 2030 TCATGA T^CATGA
5483 TCATGA
BspLU11I 2408 ACATGT A^CATGT
4557 ACATGT
BsrGI 1552 TGTACA T^GTACA
4933 TGTACA
BstXI 386 CCAACAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3051 CCAAAACGATGG
Bsu36I 296 CCTGAGG CC^T[ACGT]AGG
1052 CCTCAGG
EagI 205 CGGCCG C^GGCCG
266 CGGCCG
EclXI 205 CGGCCG C^GGCCG
266 CGGCCG
Eco52I 205 CGGCCG C^GGCCG
266 CGGCCG
Eco81I 296 CCTGAGG CC^T[ACGT]AGG
1052 CCTCAGG
EcoRI 933 GAATTC G^AATTC
3870 GAATTC
EcoT22I 1337 ATGCAT ATGCA^T
5636 ATGCAT
FbaI 2564 TGATCA T^GATCA
5281 TGATCA
HgaI 170 GACGC GACGC(5/10)
2695 GACGC
HpaI 4526 GTTAAC GTT^AAC
5120 GTTAAC
MamI 2797 GATAGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2959 GATATTAATC
NsiI 1337 ATGCAT ATGCA^T
5636 ATGCAT
PshAI 2826 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4133 GACCCTTGTC
PvuII 791 CAGCTG CAG^CTG
802 CAGCTG
RcaI 2030 TCATGA T^CATGA
5483 TCATGA
SapI 1306 GAAGAGC GCTCTTC(1/4)
1531 GAAGAGC
ScaI 2135 AGTACT AGT^ACT
4820 AGTACT
SmlI 576 CTTAAG C^T[CT][GA]AG
3196 CTTGAG
SspBI 1552 TGTACA T^GTACA
4933 TGTACA
SspI 4967 AATATT AAT^ATT
5314 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaJI BseRI BsiEI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeI
NdeII NlaIII NlaIV NspI PleI
PpuMI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII
XmaCI XmaI