##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AflII AgeI AhdI ApaI AscI
AspEI AviII AvrII BalI BamHI
BciVI BfrI BlnI BsaBI BsiWI
BspDI BspHI BssHII BssSI BstAPI
BstBI BstEII BstPI ClaI CpoI
DrdI Eam1105I EcoO65I EcoRV EcoT22I
FseI FspI HindIII HpaI MamI
MfeI MluI MluNI MscI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PvuI RcaI
RsrII SalI SfiI SfuI SgrAI
SnaBI SpeI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 4029 GACGTC GACGT^C
Acc65I 4107 GGTACC G^GTACC
AccIII 4126 TCCGGA T^CCGGA
AclI 599 AACGTT AA^CGTT
AocI 1257 CCTCAGG CC^T[ACGT]AGG
Asp718I 4107 GGTACC G^GTACC
BbeI 1607 GGCGCC GGCGC^C
BbrPI 3773 CACGTG CAC^GTG
BbvCI 962 CCTCAGC CCTCAGC(-5/-2)
BclI 4050 TGATCA T^GATCA
BglII 5024 AGATCT A^GATCT
BlpI 3398 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3398 GCTAAGC GC^T[ACGT]AGC
BseAI 4126 TCCGGA T^CCGGA
BsmBI 3870 CGTCTC CGTCTC(1/5)
BsmI 5056 GCATTC GAATGC(1/-1)
BspEI 4126 TCCGGA T^CCGGA
BspLU11I 384 ACATGT A^CATGT
BsrBI 3880 CCGCTC CCGCTC(-3/-3)
Bst1107I 2663 GTATAC GTA^TAC
BstZ17I 2663 GTATAC GTA^TAC
Bsu36I 1257 CCTCAGG CC^T[ACGT]AGG
CelII 3398 GCTAAGC GC^T[ACGT]AGC
EagI 2482 CGGCCG C^GGCCG
EclXI 2482 CGGCCG C^GGCCG
Eco52I 2482 CGGCCG C^GGCCG
Eco81I 1257 CCTCAGG CC^T[ACGT]AGG
FbaI 4050 TGATCA T^GATCA
HincII 3829 GTCAAC GT[CT]^[GA]AC
HindII 3829 GTCAAC GT[CT]^[GA]AC
KasI 1607 GGCGCC G^GCGCC
KpnI 4107 GGTACC GGTAC^C
KspI 102 CCGCGG CCGC^GG
MroI 4126 TCCGGA T^CCGGA
NarI 1607 GGCGCC GG^CGCC
PflMI 1785 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3773 CACGTG CAC^GTG
PmlI 3773 CACGTG CAC^GTG
Psp1406I 599 AACGTT AA^CGTT
SacII 102 CCGCGG CCGC^GG
SapI 5091 GAAGAGC GCTCTTC(1/4)
SbfI 2273 CCTGCAGG CCTGCA^GG
ScaI 5272 AGTACT AGT^ACT
SfoI 1607 GGCGCC GGC^GCC
SmaI 2248 CCCGGG CCC^GGG
SphI 2218 GCATGC GCATG^C
Sse8387I 2273 CCTGCAGG CCTGCA^GG
SspI 1167 AATATT AAT^ATT
StuI 1715 AGGCCT AGG^CCT
Van91I 1785 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2553 TCTAGA T^CTAGA
XmaCI 2248 CCCGGG C^CCGGG
XmaI 2248 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 1607 GGCGCC G[GA]^CG[CT]C
4029 GACGTC
Alw44I 1153 GTGCAC G^TGCAC
2672 GTGCAC
AlwNI 925 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
4036 CAGGGCCTG
Aor51HI 1367 AGCGCT AGC^GCT
1535 AGCGCT
ApaLI 1153 GTGCAC G^TGCAC
2672 GTGCAC
AseI 350 ATTAAT AT^TAAT
621 ATTAAT
AsnI 350 ATTAAT AT^TAAT
621 ATTAAT
Asp700I 425 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2804 GAATGAATTC
BbsI 1842 GAAGAC GAAGAC(2/6)
1887 GTCTTC
BcgI 2258 CGACGTGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1133 GCATATTCTTCG
BpuAI 1842 GAAGAC GAAGAC(2/6)
1887 GTCTTC
BsaAI 1323 TACGTG [CT]AC^GT[GA]
3773 CACGTG
BsaHI 1607 GGCGCC G[GA]^CG[CT]C
4029 GACGTC
BsaWI 1816 ACCGGA [AT]^CCGG[AT]
4126 TCCGGA
BsrDI 630 CATTGC GCAATG(2/0)
741 GCAATG
BsrFI 56 ACCGGC [GA]^CCGG[CT]
112 GCCGGT
BstXI 1351 CCAAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1703 CCAGGGTCCTGG
BstYI 3855 AGATCC [GA]^GATC[CT]
5024 AGATCT
Cfr10I 56 ACCGGC [GA]^CCGG[CT]
112 GCCGGT
Eco47III 1367 AGCGCT AGC^GCT
1535 AGCGCT
EcoNI 364 CCTATGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
935 CCTCAAGGAGG
Hin1I 1607 GGCGCC G[GA]^CG[CT]C
4029 GACGTC
MflI 3855 AGATCC [GA]^GATC[CT]
5024 AGATCT
NcoI 907 CCATGG C^CATGG
3379 CCATGG
PshBI 350 ATTAAT AT^TAAT
621 ATTAAT
SacI 4095 GAGCTC GAGCT^C
5094 GAGCTC
XcmI 2347 CCATGACGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2599 CCAAAACTACCTTGG
XhoII 3855 AGATCC [GA]^GATC[CT]
5024 AGATCT
XmnI 425 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2804 GAATGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI AspHI
AspI AvaI AvaII BanI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I