##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
BcgI BciVI BclI BfrI BglII
BsaBI BsiWI Bsp1407I BspDI BspHI
BsrDI BsrGI Bst1107I BstBI BstZ17I
ClaI DraI DrdI Eam1105I Eco47III
EcoRI EcoRV EcoT22I FbaI HincII
HindII HindIII HpaI KpnI MamI
MfeI MluI MunI NdeI NheI
NotI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI SalI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI Tsp509I XmnI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AatII 4694 GACGTC GACGT^C
AccIII 269 TCCGGA T^CCGGA
AspI 3699 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1212 GGATCC G^GATCC
BlpI 3195 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3195 GCTCAGC GC^T[ACGT]AGC
BseAI 269 TCCGGA T^CCGGA
BsmI 2798 GCATTC GAATGC(1/-1)
BspEI 269 TCCGGA T^CCGGA
BspLU11I 2207 ACATGT A^CATGT
BssHII 378 GCGCGC G^CGCGC
BssSI 4340 CACGAG CACGAG(-5/-1)
CelII 3195 GCTCAGC GC^T[ACGT]AGC
CpoI 4438 CGGACCG CG^G[AT]CCG
DraIII 2933 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
FseI 2555 GGCCGGCC GGCCGG^CC
MroI 269 TCCGGA T^CCGGA
NruI 1499 TCGCGA TCG^CGA
PflFI 3699 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 4438 CGGACCG CG^G[AT]CCG
SbfI 625 CCTGCAGG CCTGCA^GG
SfiI 2784 GGCCTAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 625 CCTGCAGG CCTGCA^GG
Tth111I 3699 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2471 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Alw44I 1870 GTGCAC G^TGCAC
4186 GTGCAC
AocI 978 CCTGAGG CC^T[ACGT]AGG
2525 CCTCAGG
ApaLI 1870 GTGCAC G^TGCAC
4186 GTGCAC
AviII 16 TGCGCA TGC^GCA
780 TGCGCA
AvrII 2786 CCTAGG C^CTAGG
4951 CCTAGG
BbeI 47 GGCGCC GGCGC^C
4038 GGCGCC
BbrPI 2936 CACGTG CAC^GTG
4909 CACGTG
BlnI 2786 CCTAGG C^CTAGG
4951 CCTAGG
BsaAI 2936 CACGTG [CT]AC^GT[GA]
4909 CACGTG
BsaWI 269 TCCGGA [AT]^CCGG[AT]
4436 ACCGGA
BsiEI 3822 CGGCCG CG[GA][CT]^CG
4680 CGGCCG
Bsu36I 978 CCTGAGG CC^T[ACGT]AGG
2525 CCTCAGG
EagI 3822 CGGCCG C^GGCCG
4680 CGGCCG
EclXI 3822 CGGCCG C^GGCCG
4680 CGGCCG
Eco52I 3822 CGGCCG C^GGCCG
4680 CGGCCG
Eco81I 978 CCTGAGG CC^T[ACGT]AGG
2525 CCTCAGG
FspI 16 TGCGCA TGC^GCA
780 TGCGCA
KasI 47 GGCGCC G^GCGCC
4038 GGCGCC
KspI 4006 CCGCGG CCGC^GG
5131 CCGCGG
MseI 543 TTAA T^TAA
2674 TTAA
NarI 47 GGCGCC GG^CGCC
4038 GGCGCC
PaeR7I 1985 CTCGAG C^TCGAG
4526 CTCGAG
PmaCI 2936 CACGTG CAC^GTG
4909 CACGTG
PmlI 2936 CACGTG CAC^GTG
4909 CACGTG
SacII 4006 CCGCGG CCGC^GG
5131 CCGCGG
SfoI 47 GGCGCC GGC^GCC
4038 GGCGCC
Tru9I 543 TTAA T^TAA
2674 TTAA
XhoI 1985 CTCGAG C^TCGAG
4526 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI