################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## Acc65I AccI AclI AcsI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI BcgI BciVI BclI BfrI BglII BsaBI BsiWI Bsp1407I BspDI BspHI BsrDI BsrGI Bst1107I BstBI BstZ17I ClaI DraI DrdI Eam1105I Eco47III EcoRI EcoRV EcoT22I FbaI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MunI NdeI NheI NotI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI SalI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Tsp509I XmnI ################################### # ONE CUT ENZYMES ( 25 enzymes) # ################################### AatII 4694 GACGTC GACGT^C AccIII 269 TCCGGA T^CCGGA AspI 3699 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1212 GGATCC G^GATCC BlpI 3195 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3195 GCTCAGC GC^T[ACGT]AGC BseAI 269 TCCGGA T^CCGGA BsmI 2798 GCATTC GAATGC(1/-1) BspEI 269 TCCGGA T^CCGGA BspLU11I 2207 ACATGT A^CATGT BssHII 378 GCGCGC G^CGCGC BssSI 4340 CACGAG CACGAG(-5/-1) CelII 3195 GCTCAGC GC^T[ACGT]AGC CpoI 4438 CGGACCG CG^G[AT]CCG DraIII 2933 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG FseI 2555 GGCCGGCC GGCCGG^CC MroI 269 TCCGGA T^CCGGA NruI 1499 TCGCGA TCG^CGA PflFI 3699 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC RsrII 4438 CGGACCG CG^G[AT]CCG SbfI 625 CCTGCAGG CCTGCA^GG SfiI 2784 GGCCTAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 625 CCTGCAGG CCTGCA^GG Tth111I 3699 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2471 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Alw44I 1870 GTGCAC G^TGCAC 4186 GTGCAC AocI 978 CCTGAGG CC^T[ACGT]AGG 2525 CCTCAGG ApaLI 1870 GTGCAC G^TGCAC 4186 GTGCAC AviII 16 TGCGCA TGC^GCA 780 TGCGCA AvrII 2786 CCTAGG C^CTAGG 4951 CCTAGG BbeI 47 GGCGCC GGCGC^C 4038 GGCGCC BbrPI 2936 CACGTG CAC^GTG 4909 CACGTG BlnI 2786 CCTAGG C^CTAGG 4951 CCTAGG BsaAI 2936 CACGTG [CT]AC^GT[GA] 4909 CACGTG BsaWI 269 TCCGGA [AT]^CCGG[AT] 4436 ACCGGA BsiEI 3822 CGGCCG CG[GA][CT]^CG 4680 CGGCCG Bsu36I 978 CCTGAGG CC^T[ACGT]AGG 2525 CCTCAGG EagI 3822 CGGCCG C^GGCCG 4680 CGGCCG EclXI 3822 CGGCCG C^GGCCG 4680 CGGCCG Eco52I 3822 CGGCCG C^GGCCG 4680 CGGCCG Eco81I 978 CCTGAGG CC^T[ACGT]AGG 2525 CCTCAGG FspI 16 TGCGCA TGC^GCA 780 TGCGCA KasI 47 GGCGCC G^GCGCC 4038 GGCGCC KspI 4006 CCGCGG CCGC^GG 5131 CCGCGG MseI 543 TTAA T^TAA 2674 TTAA NarI 47 GGCGCC GG^CGCC 4038 GGCGCC PaeR7I 1985 CTCGAG C^TCGAG 4526 CTCGAG PmaCI 2936 CACGTG CAC^GTG 4909 CACGTG PmlI 2936 CACGTG CAC^GTG 4909 CACGTG SacII 4006 CCGCGG CCGC^GG 5131 CCGCGG SfoI 47 GGCGCC GGC^GCC 4038 GGCGCC Tru9I 543 TTAA T^TAA 2674 TTAA XhoI 1985 CTCGAG C^TCGAG 4526 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI