##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
Aor51HI AscI Asp718I AviII AvrII
BamHI BbeI BcgI BglII BlnI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmI BspDI BspEI BssHII BssSI
BstBI BstEII BstPI ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I FseI FspI HgaI Hin1I
KasI KpnI MluI MroI NarI
NdeI NheI NotI NruI NspV
PinAI PmeI PvuI RsrII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 202 GTATAC GT^[AC][GT]AC
AclI 429 AACGTT AA^CGTT
AhdI 688 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3922 GTGCAC G^TGCAC
AocI 3103 CCTCAGG CC^T[ACGT]AGG
ApaI 1554 GGGCCC GGGCC^C
ApaLI 3922 GTGCAC G^TGCAC
AspEI 688 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 4168 CTCGAG C^[CT]CG[GA]G
BbrPI 2445 CACGTG CAC^GTG
BbvCI 87 GCTGAGG CCTCAGC(-5/-2)
BclI 1178 TGATCA T^GATCA
BcnI 2035 CCGGG CC^[GC]GG
BlpI 2545 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2545 GCTAAGC GC^T[ACGT]AGC
BsaAI 2445 CACGTG [CT]AC^GT[GA]
BsgI 3713 CTGCAC GTGCAG(16/14)
BsoBI 4168 CTCGAG C^[CT]CG[GA]G
BspLU11I 4698 ACATGT A^CATGT
BsrBI 45 GAGCGG CCGCTC(-3/-3)
Bst1107I 202 GTATAC GTA^TAC
BstZ17I 202 GTATAC GTA^TAC
Bsu36I 3103 CCTCAGG CC^T[ACGT]AGG
CelII 2545 GCTAAGC GC^T[ACGT]AGC
DraIII 1938 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 688 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3103 CCTCAGG CC^T[ACGT]AGG
EcoNI 3473 CCTTCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1903 ATGCAT ATGCA^T
FbaI 1178 TGATCA T^GATCA
HpaI 432 GTTAAC GTT^AAC
KspI 25 CCGCGG CCGC^GG
NaeI 104 GCCGGC GCC^GGC
NciI 2035 CCGGG CC^[GC]GG
NgoMIV 104 GCCGGC G^CCGGC
NsiI 1903 ATGCAT ATGCA^T
PacI 949 TTAATTAA TTAAT^TAA
PaeR7I 4168 CTCGAG C^TCGAG
PmaCI 2445 CACGTG CAC^GTG
PmlI 2445 CACGTG CAC^GTG
PshAI 2809 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 429 AACGTT AA^CGTT
PstI 898 CTGCAG CTGCA^G
SacI 2947 GAGCTC GAGCT^C
SacII 25 CCGCGG CCGC^GG
SapI 2874 GCTCTTC GCTCTTC(1/4)
ScaI 380 AGTACT AGT^ACT
SexAI 1317 ACCTGGT A^CC[AT]GGT
XhoI 4168 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 26 CGCG CG^CG
101 CGCG
AseI 952 ATTAAT AT^TAAT
3733 ATTAAT
AsnI 952 ATTAAT AT^TAAT
3733 ATTAAT
Asp700I 484 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
652 GAAGTGATTC
AspI 4293 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4328 GACCCCGTC
BanI 1168 GGCACC G^G[CT][GA]CC
2236 GGCACC
BglI 99 GCCGCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1498 GCCATAGAGGC
BsaBI 748 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4510 GATTCAGATC
BspHI 773 TCATGA T^CATGA
3881 TCATGA
BspMI 583 GCAGGT ACCTGC(4/8)
2993 GCAGGT
BsrDI 4943 CATTGC GCAATG(2/0)
306 GCAATG
BsrFI 104 GCCGGC [GA]^CCGG[CT]
226 ACCGGC
BstAPI 559 GCAAAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3715 GCACCATTTGC
BstUI 26 CGCG CG^CG
101 CGCG
BstXI 713 CCATAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
811 CCAGAGCTATGG
Cfr10I 104 GCCGGC [GA]^CCGG[CT]
226 ACCGGC
HaeII 69 AGCGCC [GA]GCGC^[CT]
1761 GGCGCT
HincII 432 GTTAAC GT[CT]^[GA]AC
587 GTCAAC
HindII 432 GTTAAC GT[CT]^[GA]AC
587 GTCAAC
HindIII 2190 AAGCTT A^AGCTT
4770 AAGCTT
MamI 748 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4510 GATTCAGATC
MfeI 1864 CAATTG C^AATTG
4904 CAATTG
MunI 1864 CAATTG C^AATTG
4904 CAATTG
MvnI 26 CGCG CG^CG
101 CGCG
PflFI 4293 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4328 GACCCCGTC
PflMI 1354 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1569 CCAGGCTTTGG
PshBI 952 ATTAAT AT^TAAT
3733 ATTAAT
RcaI 773 TCATGA T^CATGA
3881 TCATGA
Tth111I 4293 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4328 GACCCCGTC
Van91I 1354 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1569 CCAGGCTTTGG
XcmI 1565 CCACCCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2223 CCAGGAGAAGCCTGG
XmnI 484 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
652 GAAGTGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanII BbsI BbvI
BciVI BfaI BfrI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoRV EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII