##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI BamHI BcgI BciVI
BclI BsiEI BsiWI BspDI BssHII
BssSI BstEII BstPI ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRV EcoT22I FbaI FseI HpaI
KpnI KspI MfeI MluI MunI
NaeI NgoMIV NheI NotI NruI
NsiI PacI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI Sse8387I SspI
StuI SwaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 2258 GTATAC GT^[AC][GT]AC
AccII 1785 CGCG CG^CG
AcyI 1621 GGCGCC G[GA]^CG[CT]C
AlwNI 3299 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 3106 AGCGCT AGC^GCT
AspHI 4378 GAGCTC G[AT]GC[AT]^C
AvrII 3259 CCTAGG C^CTAGG
BbeI 1621 GGCGCC GGCGC^C
BbrPI 847 CACGTG CAC^GTG
BglI 3684 GCCAACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3259 CCTAGG C^CTAGG
BsaBI 2673 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1621 GGCGCC G[GA]^CG[CT]C
BsgI 3836 CTGCAC GTGCAG(16/14)
BsiHKAI 4378 GAGCTC G[AT]GC[AT]^C
BsmBI 2947 GAGACG CGTCTC(1/5)
BsmI 886 GAATGC GAATGC(1/-1)
BsrBI 867 GAGCGG CCGCTC(-3/-3)
BsrFI 1618 ACCGGC [GA]^CCGG[CT]
Bst1107I 2258 GTATAC GTA^TAC
BstBI 1111 TTCGAA TT^CGAA
BstUI 1785 CGCG CG^CG
BstZ17I 2258 GTATAC GTA^TAC
Cfr10I 1618 ACCGGC [GA]^CCGG[CT]
DraIII 362 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3106 AGCGCT AGC^GCT
Hin1I 1621 GGCGCC G[GA]^CG[CT]C
HincII 590 GTCAAC GT[CT]^[GA]AC
HindII 590 GTCAAC GT[CT]^[GA]AC
KasI 1621 GGCGCC G^GCGCC
MamI 2673 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1785 CGCG CG^CG
NarI 1621 GGCGCC GG^CGCC
NcoI 2966 CCATGG C^CATGG
NdeI 4916 CATATG CA^TATG
NspV 1111 TTCGAA TT^CGAA
PaeR7I 2470 CTCGAG C^TCGAG
PflMI 2966 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 847 CACGTG CAC^GTG
PmlI 847 CACGTG CAC^GTG
PpuMI 1226 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 474 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 172 CTGCAG CTGCA^G
SacI 4378 GAGCTC GAGCT^C
SapI 3099 GAAGAGC GCTCTTC(1/4)
ScaI 4439 AGTACT AGT^ACT
SfoI 1621 GGCGCC GGC^GCC
SfuI 1111 TTCGAA TT^CGAA
SgrAI 1617 CACCGGCG C[GA]^CCGG[CT]G
SmaI 3797 CCCGGG CCC^GGG
SpeI 3551 ACTAGT A^CTAGT
SphI 2884 GCATGC GCATG^C
Van91I 2966 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4627 TCTAGA T^CTAGA
XhoI 2470 CTCGAG C^TCGAG
XmaCI 3797 CCCGGG C^CCGGG
XmaI 3797 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 19 TCCGGA T^CCGGA
915 TCCGGA
AflIII 811 ACATGT A^C[GA][CT]GT
4419 ACATGT
AocI 531 CCTGAGG CC^T[ACGT]AGG
3655 CCTGAGG
ApaI 618 GGGCCC GGGCC^C
2412 GGGCCC
Asp700I 1104 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4520 GAAAGTCTTC
AviII 580 TGCGCA TGC^GCA
1078 TGCGCA
BanI 1296 GGTGCC G^G[CT][GA]CC
1621 GGCGCC
BbvCI 2424 CCTCAGC CCTCAGC(-5/-2)
3773 GCTGAGG
BcnI 3797 CCCGG CC^[GC]GG
3798 CCGGG
BglII 1685 AGATCT A^GATCT
1897 AGATCT
BsaI 2145 GAGACC GGTCTC(1/5)
3672 GAGACC
BsaWI 19 TCCGGA [AT]^CCGG[AT]
915 TCCGGA
BseAI 19 TCCGGA T^CCGGA
915 TCCGGA
Bsp1407I 2705 TGTACA T^GTACA
3295 TGTACA
BspEI 19 TCCGGA T^CCGGA
915 TCCGGA
BspHI 3317 TCATGA T^CATGA
4311 TCATGA
BspLU11I 811 ACATGT A^CATGT
4419 ACATGT
BspMI 4675 ACCTGC ACCTGC(4/8)
2543 GCAGGT
BsrGI 2705 TGTACA T^GTACA
3295 TGTACA
BstAPI 1290 GCATGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3805 GCAGAGGTTGC
BstXI 2061 CCAGATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4385 CCATCAAGTTGG
Bsu36I 531 CCTGAGG CC^T[ACGT]AGG
3655 CCTGAGG
Eco81I 531 CCTGAGG CC^T[ACGT]AGG
3655 CCTGAGG
EcoRI 1104 GAATTC G^AATTC
4410 GAATTC
FspI 580 TGCGCA TGC^GCA
1078 TGCGCA
HaeII 1621 GGCGCC [GA]GCGC^[CT]
3106 AGCGCT
MroI 19 TCCGGA T^CCGGA
915 TCCGGA
NciI 3797 CCCGG CC^[GC]GG
3798 CCGGG
RcaI 3317 TCATGA T^CATGA
4311 TCATGA
SnaBI 514 TACGTA TAC^GTA
2152 TACGTA
SspBI 2705 TGTACA T^GTACA
3295 TGTACA
XcmI 1456 CCAAAATTGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3173 CCACTCCATTGCTGG
XmnI 1104 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4520 GAAAGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI ApoI AspI AvaI AvaII
BalI BanII BbsI BbvI BfaI
BfrI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DrdI
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflFI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoII