##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI AhdI Aor51HI AscI Asp700I
Asp718I AspEI AviII BamHI BfrI
BglII BsiEI BsiWI BspDI BsrBI
BssHII Bst1107I BstBI BstUI BstYI
BstZ17I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoT22I
FseI FspI HpaI KpnI KspI
MfeI MflI MluI MunI MvnI
NdeI NheI NotI NruI NsiI
NspV PaeR7I PinAI PmeI PshAI
Psp1406I PvuI RsrII SacII SapI
SbfI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XhoI XhoII
XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccIII 3327 TCCGGA T^CCGGA
Alw44I 4157 GTGCAC G^TGCAC
ApaLI 4157 GTGCAC G^TGCAC
AspI 4279 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 166 CCTAGG C^CTAGG
BbrPI 3214 CACGTG CAC^GTG
BclI 4240 TGATCA T^GATCA
BlnI 166 CCTAGG C^CTAGG
BsaAI 3214 CACGTG [CT]AC^GT[GA]
BsaBI 3988 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3327 TCCGGA [AT]^CCGG[AT]
BseAI 3327 TCCGGA T^CCGGA
BsmBI 1444 GAGACG CGTCTC(1/5)
Bsp1407I 2596 TGTACA T^GTACA
BspEI 3327 TCCGGA T^CCGGA
BspHI 2605 TCATGA T^CATGA
BspLU11I 4720 ACATGT A^CATGT
BsrDI 2160 CATTGC GCAATG(2/0)
BsrGI 2596 TGTACA T^GTACA
BssSI 4929 CTCGTG CACGAG(-5/-1)
BstAPI 805 GCAGGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1863 GGTGACC G^GT[ACGT]ACC
BstPI 1863 GGTGACC G^GT[ACGT]ACC
DraI 3426 TTTAAA TTT^AAA
DrdI 4657 GACCTCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1863 GGTGACC G^GT[ACGT]ACC
EcoRV 1944 GATATC GAT^ATC
FbaI 4240 TGATCA T^GATCA
HindIII 2323 AAGCTT A^AGCTT
MamI 3988 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3327 TCCGGA T^CCGGA
NaeI 361 GCCGGC GCC^GGC
NgoMIV 361 GCCGGC G^CCGGC
PacI 3453 TTAATTAA TTAAT^TAA
PflFI 4279 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2445 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3214 CACGTG CAC^GTG
PmlI 3214 CACGTG CAC^GTG
RcaI 2605 TCATGA T^CATGA
SalI 1684 GTCGAC G^TCGAC
ScaI 1023 AGTACT AGT^ACT
SexAI 4391 ACCTGGT A^CC[AT]GGT
SfiI 2217 GGCCTCAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 376 CCCGGG CCC^GGG
SphI 2801 GCATGC GCATG^C
SspBI 2596 TGTACA T^GTACA
TaqI 1685 TCGA T^CGA
Tth111I 4279 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1685 TCGA T^CGA
Van91I 2445 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3440 TCTAGA T^CTAGA
XmaCI 376 CCCGGG C^CCGGG
XmaI 376 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 3215 ACGTGT A^C[GA][CT]GT
4720 ACATGT
AseI 3407 ATTAAT AT^TAAT
3452 ATTAAT
AsnI 3407 ATTAAT AT^TAAT
3452 ATTAAT
BalI 4646 TGGCCA TGG^CCA
5004 TGGCCA
BcgI 2816 CGATAAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5100 GCAGGAGAATCG
BlpI 2059 GCTCAGC GC^T[ACGT]AGC
2568 GCTGAGC
Bpu1102I 2059 GCTCAGC GC^T[ACGT]AGC
2568 GCTGAGC
BsmI 276 GAATGC GAATGC(1/-1)
2781 GCATTC
CelII 2059 GCTCAGC GC^T[ACGT]AGC
2568 GCTGAGC
DraIII 3211 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
4501 CACGGTGTG
EcoNI 3012 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3822 CCTAAAACAGG
HgaI 1446 GACGC GACGC(5/10)
4425 GCGTC
HincII 1684 GTCGAC GT[CT]^[GA]AC
2236 GTTGAC
HindII 1684 GTCGAC GT[CT]^[GA]AC
2236 GTTGAC
MluNI 4646 TGGCCA TGG^CCA
5004 TGGCCA
MscI 4646 TGGCCA TGG^CCA
5004 TGGCCA
PshBI 3407 ATTAAT AT^TAAT
3452 ATTAAT
SacI 1785 GAGCTC GAGCT^C
2361 GAGCTC
XcmI 1898 CCAAAGCCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2082 CCAGCCTTCAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BanI
BanII BbeI BbsI BbvCI BbvI
BciVI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstXI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI