################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII Acc65I AccII AclI AflII AgeI AhdI Aor51HI AscI Asp700I Asp718I AspEI AviII BamHI BfrI BglII BsiEI BsiWI BspDI BsrBI BssHII Bst1107I BstBI BstUI BstYI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoT22I FseI FspI HpaI KpnI KspI MfeI MflI MluI MunI MvnI NdeI NheI NotI NruI NsiI NspV PaeR7I PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SapI SbfI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccIII 3327 TCCGGA T^CCGGA Alw44I 4157 GTGCAC G^TGCAC ApaLI 4157 GTGCAC G^TGCAC AspI 4279 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 166 CCTAGG C^CTAGG BbrPI 3214 CACGTG CAC^GTG BclI 4240 TGATCA T^GATCA BlnI 166 CCTAGG C^CTAGG BsaAI 3214 CACGTG [CT]AC^GT[GA] BsaBI 3988 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 3327 TCCGGA [AT]^CCGG[AT] BseAI 3327 TCCGGA T^CCGGA BsmBI 1444 GAGACG CGTCTC(1/5) Bsp1407I 2596 TGTACA T^GTACA BspEI 3327 TCCGGA T^CCGGA BspHI 2605 TCATGA T^CATGA BspLU11I 4720 ACATGT A^CATGT BsrDI 2160 CATTGC GCAATG(2/0) BsrGI 2596 TGTACA T^GTACA BssSI 4929 CTCGTG CACGAG(-5/-1) BstAPI 805 GCAGGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1863 GGTGACC G^GT[ACGT]ACC BstPI 1863 GGTGACC G^GT[ACGT]ACC DraI 3426 TTTAAA TTT^AAA DrdI 4657 GACCTCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1863 GGTGACC G^GT[ACGT]ACC EcoRV 1944 GATATC GAT^ATC FbaI 4240 TGATCA T^GATCA HindIII 2323 AAGCTT A^AGCTT MamI 3988 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 3327 TCCGGA T^CCGGA NaeI 361 GCCGGC GCC^GGC NgoMIV 361 GCCGGC G^CCGGC PacI 3453 TTAATTAA TTAAT^TAA PflFI 4279 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2445 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3214 CACGTG CAC^GTG PmlI 3214 CACGTG CAC^GTG RcaI 2605 TCATGA T^CATGA SalI 1684 GTCGAC G^TCGAC ScaI 1023 AGTACT AGT^ACT SexAI 4391 ACCTGGT A^CC[AT]GGT SfiI 2217 GGCCTCAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 376 CCCGGG CCC^GGG SphI 2801 GCATGC GCATG^C SspBI 2596 TGTACA T^GTACA TaqI 1685 TCGA T^CGA Tth111I 4279 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1685 TCGA T^CGA Van91I 2445 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3440 TCTAGA T^CTAGA XmaCI 376 CCCGGG C^CCGGG XmaI 376 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflIII 3215 ACGTGT A^C[GA][CT]GT 4720 ACATGT AseI 3407 ATTAAT AT^TAAT 3452 ATTAAT AsnI 3407 ATTAAT AT^TAAT 3452 ATTAAT BalI 4646 TGGCCA TGG^CCA 5004 TGGCCA BcgI 2816 CGATAAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5100 GCAGGAGAATCG BlpI 2059 GCTCAGC GC^T[ACGT]AGC 2568 GCTGAGC Bpu1102I 2059 GCTCAGC GC^T[ACGT]AGC 2568 GCTGAGC BsmI 276 GAATGC GAATGC(1/-1) 2781 GCATTC CelII 2059 GCTCAGC GC^T[ACGT]AGC 2568 GCTGAGC DraIII 3211 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG 4501 CACGGTGTG EcoNI 3012 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3822 CCTAAAACAGG HgaI 1446 GACGC GACGC(5/10) 4425 GCGTC HincII 1684 GTCGAC GT[CT]^[GA]AC 2236 GTTGAC HindII 1684 GTCGAC GT[CT]^[GA]AC 2236 GTTGAC MluNI 4646 TGGCCA TGG^CCA 5004 TGGCCA MscI 4646 TGGCCA TGG^CCA 5004 TGGCCA PshBI 3407 ATTAAT AT^TAAT 3452 ATTAAT SacI 1785 GAGCTC GAGCT^C 2361 GAGCTC XcmI 1898 CCAAAGCCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2082 CCAGCCTTCAAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BanI BanII BbeI BbsI BbvCI BbvI BciVI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstXI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI