##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI Asp718I
AvrII BalI BamHI BcgI BfrI
BglI BlnI BsaBI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
BstBI ClaI CpoI DraIII DrdI
Eco47III EcoT22I FseI HindIII HpaI
KpnI MamI MluNI MroI MscI
NaeI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PflMI
PinAI PmeI PshAI Psp1406I PvuI
RcaI RsrII SalI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspBI StuI SwaI Van91I XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 2579 GACGTC GACGT^C
AflIII 993 ACGCGT A^C[GA][CT]GT
AhdI 4210 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 730 GGATC GGATC(4/5)
AlwNI 2334 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 4210 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3481 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3123 TGCGCA TGC^GCA
BbrPI 3227 CACGTG CAC^GTG
BbvCI 1206 GCTGAGG CCTCAGC(-5/-2)
BglII 4200 AGATCT A^GATCT
BsaAI 3227 CACGTG [CT]AC^GT[GA]
BsmI 3961 GAATGC GAATGC(1/-1)
BsrFI 461 ACCGGC [GA]^CCGG[CT]
Bst1107I 3974 GTATAC GTA^TAC
BstAPI 1288 GCAAGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 4200 AGATCT [GA]^GATC[CT]
BstZ17I 3974 GTATAC GTA^TAC
Cfr10I 461 ACCGGC [GA]^CCGG[CT]
Eam1105I 4210 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1929 CCTTGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4259 GATATC GAT^ATC
FspI 3123 TGCGCA TGC^GCA
HincII 2708 GTCAAC GT[CT]^[GA]AC
HindII 2708 GTCAAC GT[CT]^[GA]AC
KspI 35 CCGCGG CCGC^GG
MflI 4200 AGATCT [GA]^GATC[CT]
MluI 993 ACGCGT A^CGCGT
MslI 2560 CATAACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 4093 CATATG CA^TATG
NspI 3826 GCATGT [GA]CATG^[CT]
PaeR7I 1412 CTCGAG C^TCGAG
PflFI 3481 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3227 CACGTG CAC^GTG
PmlI 3227 CACGTG CAC^GTG
SacII 35 CCGCGG CCGC^GG
SbfI 2821 CCTGCAGG CCTGCA^GG
Sse8387I 2821 CCTGCAGG CCTGCA^GG
Tth111I 3481 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1412 CTCGAG C^TCGAG
XhoII 4200 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
ApaI 617 GGGCCC GGGCC^C
3439 GGGCCC
AseI 1955 ATTAAT AT^TAAT
2132 ATTAAT
AsnI 1955 ATTAAT AT^TAAT
2132 ATTAAT
Asp700I 1119 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2108 GAAGATATTC
BbeI 24 GGCGCC GGCGC^C
2846 GGCGCC
BciVI 2358 GGATAC GTATCC(6/5)
3781 GGATAC
BclI 1633 TGATCA T^GATCA
3724 TGATCA
BlpI 317 GCTGAGC GC^T[ACGT]AGC
3139 GCTGAGC
Bpu1102I 317 GCTGAGC GC^T[ACGT]AGC
3139 GCTGAGC
BsmBI 2041 CGTCTC CGTCTC(1/5)
990 GAGACG
BstEII 185 GGTGACC G^GT[ACGT]ACC
3007 GGTGACC
BstPI 185 GGTGACC G^GT[ACGT]ACC
3007 GGTGACC
BstXI 190 CCAAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3012 CCAAGGTGGTGG
CelII 317 GCTGAGC GC^T[ACGT]AGC
3139 GCTGAGC
EagI 166 CGGCCG C^GGCCG
2988 CGGCCG
EclXI 166 CGGCCG C^GGCCG
2988 CGGCCG
Eco52I 166 CGGCCG C^GGCCG
2988 CGGCCG
EcoO65I 185 GGTGACC G^GT[ACGT]ACC
3007 GGTGACC
EcoRI 713 GAATTC G^AATTC
2253 GAATTC
FbaI 1633 TGATCA T^GATCA
3724 TGATCA
KasI 24 GGCGCC G^GCGCC
2846 GGCGCC
MfeI 692 CAATTG C^AATTG
2184 CAATTG
MunI 692 CAATTG C^AATTG
2184 CAATTG
NarI 24 GGCGCC GG^CGCC
2846 GGCGCC
PshBI 1955 ATTAAT AT^TAAT
2132 ATTAAT
SfoI 24 GGCGCC GGC^GCC
2846 GGCGCC
SspI 915 AATATT AAT^ATT
3925 AATATT
XcmI 337 CCAAGCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3159 CCAAGCACAGGCTGG
XmnI 1119 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2108 GAAGATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AocI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmaCI XmaI