##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
Aor51HI AscI Asp718I AspI AvaI
AviII AvrII BanI BbeI BcgI
BciVI BcnI BglI BlnI BsaHI
BsaI BsgI BsiEI BsiWI BsoBI
BspDI BspHI BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
Cfr10I ClaI CpoI DrdI DsaI
EagI EclXI Eco47III Eco52I FauI
FseI FspI Hin1I KasI KpnI
KspI MluI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NspV PacI PaeR7I PflFI PinAI
PmeI Psp1406I PvuI RcaI RsrII
SacII SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 1409 GTCGAC GT^[AC][GT]AC
AccII 4525 CGCG CG^CG
AflII 3778 CTTAAG C^TTAAG
AhdI 1033 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2637 CCTGAGG CC^T[ACGT]AGG
ApaI 3931 GGGCCC GGGCC^C
AspEI 1033 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 3261 TGGCCA TGG^CCA
BamHI 2999 GGATCC G^GATCC
BbrPI 4302 CACGTG CAC^GTG
BclI 3398 TGATCA T^GATCA
BfrI 3778 CTTAAG C^TTAAG
BlpI 481 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 481 GCTGAGC GC^T[ACGT]AGC
BsaBI 2913 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 4284 GAGACG CGTCTC(1/5)
Bsp1407I 2810 TGTACA T^GTACA
BsrGI 2810 TGTACA T^GTACA
BstEII 2829 GGTTACC G^GT[ACGT]ACC
BstPI 2829 GGTTACC G^GT[ACGT]ACC
BstUI 4525 CGCG CG^CG
Bsu36I 2637 CCTGAGG CC^T[ACGT]AGG
CelII 481 GCTGAGC GC^T[ACGT]AGC
DraIII 3004 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 3261 TGGCCA [CT]^GGCC[GA]
Eam1105I 1033 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2637 CCTGAGG CC^T[ACGT]AGG
EcoNI 4419 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2829 GGTTACC G^GT[ACGT]ACC
EcoRI 3490 GAATTC G^AATTC
FbaI 3398 TGATCA T^GATCA
HaeII 4184 AGCGCC [GA]GCGC^[CT]
HgaI 4480 GACGC GACGC(5/10)
HindIII 872 AAGCTT A^AGCTT
HpaI 3993 GTTAAC GTT^AAC
MamI 2913 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3261 TGGCCA TGG^CCA
MscI 3261 TGGCCA TGG^CCA
MvnI 4525 CGCG CG^CG
NruI 4524 TCGCGA TCG^CGA
PflMI 3003 CCACGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4302 CACGTG CAC^GTG
PmlI 4302 CACGTG CAC^GTG
PshAI 5312 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1409 GTCGAC G^TCGAC
SapI 790 GAAGAGC GCTCTTC(1/4)
SexAI 1809 ACCTGGT A^CC[AT]GGT
SpeI 2973 ACTAGT A^CTAGT
SphI 2581 GCATGC GCATG^C
SspBI 2810 TGTACA T^GTACA
SwaI 5452 ATTTAAAT ATTT^AAAT
Van91I 3003 CCACGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccIII 927 TCCGGA T^CCGGA
2798 TCCGGA
Alw44I 1141 GTGCAC G^TGCAC
3877 GTGCAC
ApaLI 1141 GTGCAC G^TGCAC
3877 GTGCAC
BbsI 634 GAAGAC GAAGAC(2/6)
676 GAAGAC
BbvCI 2403 GCTGAGG CCTCAGC(-5/-2)
3921 GCTGAGG
BpuAI 634 GAAGAC GAAGAC(2/6)
676 GAAGAC
BsaAI 171 TACGTG [CT]AC^GT[GA]
4302 CACGTG
BseAI 927 TCCGGA T^CCGGA
2798 TCCGGA
BsmI 2721 GAATGC GAATGC(1/-1)
1622 GCATTC
BspEI 927 TCCGGA T^CCGGA
2798 TCCGGA
BstAPI 2679 GCAAGCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4246 GCACCTAATGC
EcoT14I 1444 CCAAGG C^C[AT][AT]GG
4598 CCAAGG
EcoT22I 2686 ATGCAT ATGCA^T
2723 ATGCAT
MfeI 355 CAATTG C^AATTG
4358 CAATTG
MroI 927 TCCGGA T^CCGGA
2798 TCCGGA
MunI 355 CAATTG C^AATTG
4358 CAATTG
NsiI 2686 ATGCAT ATGCA^T
2723 ATGCAT
PpuMI 4318 AGGTCCT [GA]G^G[AT]CC[CT]
5272 GGGTCCT
SacI 1591 GAGCTC GAGCT^C
1642 GAGCTC
StyI 1444 CCAAGG C^C[AT][AT]GG
4598 CCAAGG
XbaI 2046 TCTAGA T^CTAGA
4397 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BanII BbvI
BfaI BglII BmyI BpmI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspLU11I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII EcoRV Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
StuI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI