##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AflII Aor51HI AscI
AviII BbeI BbrPI BcgI BciVI
BfrI BlpI Bpu1102I BsaAI BseAI
BsiWI BspDI BspEI BsrBI BsrDI
BssHII BstBI BstEII BstPI CelII
ClaI CpoI DrdI Eco47III EcoO65I
EcoRV EcoT22I FseI FspI HaeII
KasI MluI MroI NarI NotI
NruI NsiI NspV PacI PmaCI
PmlI PshAI PvuI RsrII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I StuI SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 5721 GGTACC G^GTACC
AclI 3335 AACGTT AA^CGTT
AcyI 5575 GACGCC G[GA]^CG[CT]C
AgeI 3177 ACCGGT A^CCGGT
Alw44I 1926 GTGCAC G^TGCAC
ApaLI 1926 GTGCAC G^TGCAC
AseI 4031 ATTAAT AT^TAAT
AsnI 4031 ATTAAT AT^TAAT
Asp718I 5721 GGTACC G^GTACC
AvaI 2196 CTCGAG C^[CT]CG[GA]G
AvrII 4541 CCTAGG C^CTAGG
BamHI 4108 GGATCC G^GATCC
BglI 1816 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4541 CCTAGG C^CTAGG
BsaBI 605 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5575 GACGCC G[GA]^CG[CT]C
BsaWI 3177 ACCGGT [AT]^CCGG[AT]
BsiEI 96 CGGCCG CG[GA][CT]^CG
BsoBI 2196 CTCGAG C^[CT]CG[GA]G
Bsp1407I 4943 TGTACA T^GTACA
BspHI 2073 TCATGA T^CATGA
BsrGI 4943 TGTACA T^GTACA
Bst1107I 4148 GTATAC GTA^TAC
BstAPI 2771 GCAGAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 4148 GTATAC GTA^TAC
DraIII 455 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 96 CGGCCG C^GGCCG
EclXI 96 CGGCCG C^GGCCG
Eco52I 96 CGGCCG C^GGCCG
EcoNI 3154 CCTGTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 5575 GACGCC G[GA]^CG[CT]C
HindIII 986 AAGCTT A^AGCTT
KpnI 5721 GGTACC GGTAC^C
MamI 605 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 162 GCCGGC GCC^GGC
NgoMIV 162 GCCGGC G^CCGGC
NheI 2413 GCTAGC G^CTAGC
PaeR7I 2196 CTCGAG C^TCGAG
PinAI 3177 ACCGGT A^CCGGT
PmeI 3471 GTTTAAAC GTTT^AAAC
PshBI 4031 ATTAAT AT^TAAT
Psp1406I 3335 AACGTT AA^CGTT
RcaI 2073 TCATGA T^CATGA
ScaI 4399 AGTACT AGT^ACT
SpeI 156 ACTAGT A^CTAGT
SphI 4281 GCATGC GCATG^C
SspBI 4943 TGTACA T^GTACA
XhoI 2196 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 2670 GTCTAC GT^[AC][GT]AC
4148 GTATAC
AccII 2173 CGCG CG^CG
3646 CGCG
AhdI 2576 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5503 GACTAGATGTC
AocI 2641 CCTGAGG CC^T[ACGT]AGG
3894 CCTAAGG
ApaI 2177 GGGCCC GGGCC^C
5102 GGGCCC
AspEI 2576 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5503 GACTAGATGTC
AspI 4471 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5599 GACGGAGTC
BsmBI 5597 GAGACG CGTCTC(1/5)
5768 GAGACG
BsrFI 162 GCCGGC [GA]^CCGG[CT]
3177 ACCGGT
BssSI 77 CTCGTG CACGAG(-5/-1)
5815 CTCGTG
BstUI 2173 CGCG CG^CG
3646 CGCG
Bsu36I 2641 CCTGAGG CC^T[ACGT]AGG
3894 CCTAAGG
Cfr10I 162 GCCGGC [GA]^CCGG[CT]
3177 ACCGGT
Eam1105I 2576 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5503 GACTAGATGTC
Eco81I 2641 CCTGAGG CC^T[ACGT]AGG
3894 CCTAAGG
FauI 4381 CCCGC CCCGC(4/6)
2174 GCGGG
HgaI 3253 GACGC GACGC(5/10)
5575 GACGC
HpaI 673 GTTAAC GTT^AAC
4891 GTTAAC
KspI 2172 CCGCGG CCGC^GG
3645 CCGCGG
MvnI 2173 CGCG CG^CG
3646 CGCG
NdeI 4122 CATATG CA^TATG
5989 CATATG
PflFI 4471 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5599 GACGGAGTC
PflMI 2422 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4228 CCACCTCCTGG
PpuMI 1799 GGGACCC [GA]G^G[AT]CC[CT]
2637 AGGTCCT
SacI 447 GAGCTC GAGCT^C
1773 GAGCTC
SacII 2172 CCGCGG CCGC^GG
3645 CCGCGG
SapI 731 GAAGAGC GCTCTTC(1/4)
1367 GAAGAGC
Tth111I 4471 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5599 GACGGAGTC
Van91I 2422 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4228 CCACCTCCTGG
XbaI 2362 TCTAGA T^CTAGA
2824 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BglII BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspLU11I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MunI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI