##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccI AccIII AflII AgeI
ApaI AscI Asp700I AviII BamHI
BcgI BfrI BsaWI BseAI BsiWI
BspDI BspEI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DrdI EagI EclXI Eco52I EcoO65I
EcoRI EcoRV FseI FspI HindIII
HpaI KspI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmeI PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI Van91I XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 3746 GGTACC G^GTACC
AhdI 3605 GACGTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1209 CCTGAGG CC^T[ACGT]AGG
AseI 4458 ATTAAT AT^TAAT
AsnI 4458 ATTAAT AT^TAAT
Asp718I 3746 GGTACC G^GTACC
AspEI 3605 GACGTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1412 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 305 CTCGGG C^[CT]CG[GA]G
AvrII 937 CCTAGG C^CTAGG
BciVI 1061 GTATCC GTATCC(6/5)
BcnI 20 CCCGG CC^[GC]GG
BglII 3146 AGATCT A^GATCT
BlnI 937 CCTAGG C^CTAGG
BsaBI 4131 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1501 CGGTCG CG[GA][CT]^CG
BsoBI 305 CTCGGG C^[CT]CG[GA]G
BspHI 1838 TCATGA T^CATGA
BspLU11I 958 ACATGT A^CATGT
BstAPI 2619 GCAGGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1209 CCTGAGG CC^T[ACGT]AGG
Eam1105I 3605 GACGTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1209 CCTGAGG CC^T[ACGT]AGG
HincII 5092 GTTGAC GT[CT]^[GA]AC
HindII 5092 GTTGAC GT[CT]^[GA]AC
KpnI 3746 GGTACC GGTAC^C
MamI 4131 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 20 CCCGG CC^[GC]GG
NcoI 852 CCATGG C^CATGG
PflFI 1412 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 4458 ATTAAT AT^TAAT
RcaI 1838 TCATGA T^CATGA
SapI 2352 GCTCTTC GCTCTTC(1/4)
Tth111I 1412 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3686 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AclI 3804 AACGTT AA^CGTT
4327 AACGTT
AcsI 1125 GAATTT [GA]^AATT[CT]
3104 AAATTT
Alw44I 3832 GTGCAC G^TGCAC
4241 GTGCAC
Aor51HI 3406 AGCGCT AGC^GCT
4916 AGCGCT
ApaLI 3832 GTGCAC G^TGCAC
4241 GTGCAC
ApoI 1125 GAATTT [GA]^AATT[CT]
3104 AAATTT
BbeI 2302 GGCGCC GGCGC^C
2398 GGCGCC
BbrPI 1294 CACGTG CAC^GTG
4953 CACGTG
BbsI 1536 GTCTTC GAAGAC(2/6)
5085 GTCTTC
BbvCI 1324 GCTGAGG CCTCAGC(-5/-2)
2980 GCTGAGG
BlpI 229 GCTAAGC GC^T[ACGT]AGC
765 GCTCAGC
Bpu1102I 229 GCTAAGC GC^T[ACGT]AGC
765 GCTCAGC
BpuAI 1536 GTCTTC GAAGAC(2/6)
5085 GTCTTC
BsaI 3369 GAGACC GGTCTC(1/5)
1529 GGTCTC
BsmI 3304 GAATGC GAATGC(1/-1)
4667 GAATGC
BsrDI 5203 CATTGC GCAATG(2/0)
1579 GCAATG
BsrFI 3318 GCCGGT [GA]^CCGG[CT]
3673 GCCGGT
CelII 229 GCTAAGC GC^T[ACGT]AGC
765 GCTCAGC
Cfr10I 3318 GCCGGT [GA]^CCGG[CT]
3673 GCCGGT
Eco47III 3406 AGCGCT AGC^GCT
4916 AGCGCT
EcoT22I 1561 ATGCAT ATGCA^T
5200 ATGCAT
KasI 2302 GGCGCC G^GCGCC
2398 GGCGCC
NarI 2302 GGCGCC GG^CGCC
2398 GGCGCC
NsiI 1561 ATGCAT ATGCA^T
5200 ATGCAT
PmaCI 1294 CACGTG CAC^GTG
4953 CACGTG
PmlI 1294 CACGTG CAC^GTG
4953 CACGTG
PshAI 642 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1753 GACCCCAGTC
Psp1406I 3804 AACGTT AA^CGTT
4327 AACGTT
SacI 763 GAGCTC GAGCT^C
1887 GAGCTC
SexAI 1081 ACCAGGT A^CC[AT]GGT
2309 ACCTGGT
SfoI 2302 GGCGCC GGC^GCC
2398 GGCGCC
TaqI 4301 TCGA T^CGA
4400 TCGA
TthHB8I 4301 TCGA T^CGA
4400 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AvaII
BalI BanI BanII BbvI BclI
BfaI BglI BmyI BpmI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I Bsp1407I BspMI BsrBI BsrGI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspBI SspI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII