##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AflII AgeI AhdI
Asp700I Asp718I AspEI AspI AviII
BamHI BbrPI BclI BfrI BsaBI
BsiWI BspDI BspHI BstBI ClaI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoRI EcoT22I FbaI FseI
FspI HpaI KpnI MamI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PvuI RcaI SalI ScaI SfuI
SgrAI SnaBI SpeI SwaI Tth111I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 1517 GTATAC GT^[AC][GT]AC
AccIII 3679 TCCGGA T^CCGGA
AclI 126 AACGTT AA^CGTT
AcsI 5159 GAATTT [GA]^AATT[CT]
Alw44I 3392 GTGCAC G^TGCAC
Aor51HI 50 AGCGCT AGC^GCT
ApaLI 3392 GTGCAC G^TGCAC
ApoI 5159 GAATTT [GA]^AATT[CT]
AscI 622 GGCGCGCC GG^CGCGCC
AseI 1477 ATTAAT AT^TAAT
AsnI 1477 ATTAAT AT^TAAT
AvrII 682 CCTAGG C^CTAGG
BbvCI 2315 GCTGAGG CCTCAGC(-5/-2)
BcgI 70 CGACTGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1614 AGATCT A^GATCT
BlnI 682 CCTAGG C^CTAGG
BseAI 3679 TCCGGA T^CCGGA
BsiEI 3759 CGGTCG CG[GA][CT]^CG
Bsp1407I 1826 TGTACA T^GTACA
BspEI 3679 TCCGGA T^CCGGA
BspLU11I 5026 ACATGT A^CATGT
BsrDI 4408 CATTGC GCAATG(2/0)
BsrGI 1826 TGTACA T^GTACA
Bst1107I 1517 GTATAC GTA^TAC
BstEII 4123 GGTCACC G^GT[ACGT]ACC
BstPI 4123 GGTCACC G^GT[ACGT]ACC
BstZ17I 1517 GTATAC GTA^TAC
CpoI 434 CGGTCCG CG^G[AT]CCG
Eco47III 50 AGCGCT AGC^GCT
EcoNI 83 CCTTGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4123 GGTCACC G^GT[ACGT]ACC
EcoRV 1260 GATATC GAT^ATC
HincII 129 GTTGAC GT[CT]^[GA]AC
HindII 129 GTTGAC GT[CT]^[GA]AC
HindIII 1817 AAGCTT A^AGCTT
MluI 550 ACGCGT A^CGCGT
MroI 3679 TCCGGA T^CCGGA
NcoI 1911 CCATGG C^CATGG
NdeI 1274 CATATG CA^TATG
NheI 170 GCTAGC G^CTAGC
PshBI 1477 ATTAAT AT^TAAT
Psp1406I 126 AACGTT AA^CGTT
RsrII 434 CGGTCCG CG^G[AT]CCG
SexAI 3396 ACCAGGT A^CC[AT]GGT
SmlI 3654 CTCAAG C^T[CT][GA]AG
SphI 2249 GCATGC GCATG^C
SspBI 1826 TGTACA T^GTACA
SspI 1591 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BciVI 2822 GGATAC GTATCC(6/5)
4455 GGATAC
BsaAI 2540 TACGTG [CT]AC^GT[GA]
3269 CACGTA
BsaI 2288 GAGACC GGTCTC(1/5)
3533 GAGACC
BsaWI 2030 ACCGGA [AT]^CCGG[AT]
3679 TCCGGA
BsmI 2893 GAATGC GAATGC(1/-1)
5102 GCATTC
BssHII 623 GCGCGC G^CGCGC
643 GCGCGC
BssSI 1154 CACGAG CACGAG(-5/-1)
2835 CACGAG
PflMI 1426 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4639 CCAGCTGGTGG
PshAI 3869 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5007 GACACTGGTC
SacI 1031 GAGCTC GAGCT^C
3314 GAGCTC
SbfI 536 CCTGCAGG CCTGCA^GG
4684 CCTGCAGG
Sse8387I 536 CCTGCAGG CCTGCA^GG
4684 CCTGCAGG
Van91I 1426 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4639 CCAGCTGGTGG
XbaI 1182 TCTAGA T^CTAGA
1617 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacII SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SfoI SmaI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI