##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AflII AgeI Aor51HI ApaI
AscI AviII BbrPI BciVI BfrI
BlpI Bpu1102I BsaAI BsiEI BsiWI
BsrFI BssHII Bst1107I BstBI BstZ17I
CelII Cfr10I CpoI DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoRV
FseI FspI KspI MfeI MluI
MunI NaeI NgoMIV NotI NruI
NspV PaeR7I PinAI PmaCI PmlI
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I StuI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 290 GGTACC G^GTACC
AccII 2895 CGCG CG^CG
AclI 979 AACGTT AA^CGTT
AflIII 5200 ACATGT A^C[GA][CT]GT
AhdI 4225 GACCTAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 5366 ATTAAT AT^TAAT
AsnI 5366 ATTAAT AT^TAAT
Asp700I 415 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 290 GGTACC G^GTACC
AspEI 4225 GACCTAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3086 CCTAGG C^CTAGG
BbeI 4913 GGCGCC GGCGC^C
BcgI 4947 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 4859 GCCAACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3086 CCTAGG C^CTAGG
BsaI 5458 GAGACC GGTCTC(1/5)
Bsp1407I 196 TGTACA T^GTACA
BspDI 3154 ATCGAT AT^CGAT
BspLU11I 5200 ACATGT A^CATGT
BspMI 271 GCAGGT ACCTGC(4/8)
BsrBI 11 GAGCGG CCGCTC(-3/-3)
BsrGI 196 TGTACA T^GTACA
BstUI 2895 CGCG CG^CG
ClaI 3154 ATCGAT AT^CGAT
Eam1105I 4225 GACCTAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 4913 GGCGCC [GA]GCGC^[CT]
HpaI 2562 GTTAAC GTT^AAC
KasI 4913 GGCGCC G^GCGCC
KpnI 290 GGTACC GGTAC^C
MvnI 2895 CGCG CG^CG
NarI 4913 GGCGCC GG^CGCC
NheI 4596 GCTAGC G^CTAGC
PacI 2010 TTAATTAA TTAAT^TAA
PmeI 3880 GTTTAAAC GTTT^AAAC
PshAI 1548 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5366 ATTAAT AT^TAAT
Psp1406I 979 AACGTT AA^CGTT
SexAI 1222 ACCAGGT A^CC[AT]GGT
SfoI 4913 GGCGCC GGC^GCC
SmaI 4965 CCCGGG CCC^GGG
SspBI 196 TGTACA T^GTACA
XbaI 353 TCTAGA T^CTAGA
XmaCI 4965 CCCGGG C^CCGGG
XmaI 4965 CCCGGG C^CCGGG
XmnI 415 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AocI 4830 CCTGAGG CC^T[ACGT]AGG
5210 CCTCAGG
AspI 2688 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5031 GACTCCGTC
BamHI 3426 GGATCC G^GATCC
4745 GGATCC
BclI 436 TGATCA T^GATCA
2996 TGATCA
BsaBI 1053 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3786 GATACAGATC
BspHI 3149 TCATGA T^CATGA
3489 TCATGA
BsrDI 1039 CATTGC GCAATG(2/0)
1504 GCAATG
BstAPI 178 GCATATTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4973 GCAGAGATTGC
BstEII 2753 GGTCACC G^GT[ACGT]ACC
3802 GGTTACC
BstPI 2753 GGTCACC G^GT[ACGT]ACC
3802 GGTTACC
Bsu36I 4830 CCTGAGG CC^T[ACGT]AGG
5210 CCTCAGG
CfoI 790 GCGC GCG^C
4914 GCGC
DsaI 1400 CCATGG C^C[GA][CT]GG
2801 CCATGG
Eco81I 4830 CCTGAGG CC^T[ACGT]AGG
5210 CCTCAGG
EcoO65I 2753 GGTCACC G^GT[ACGT]ACC
3802 GGTTACC
EcoT22I 176 ATGCAT ATGCA^T
687 ATGCAT
FbaI 436 TGATCA T^GATCA
2996 TGATCA
HgaI 53 GCGTC GACGC(5/10)
62 GCGTC
HhaI 790 GCGC GCG^C
4914 GCGC
HinP1I 790 GCGC G^CGC
4914 GCGC
HindIII 2417 AAGCTT A^AGCTT
5112 AAGCTT
MamI 1053 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3786 GATACAGATC
NcoI 1400 CCATGG C^CATGG
2801 CCATGG
NdeI 1198 CATATG CA^TATG
3824 CATATG
NsiI 176 ATGCAT ATGCA^T
687 ATGCAT
PflFI 2688 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5031 GACTCCGTC
PstI 3442 CTGCAG CTGCA^G
4473 CTGCAG
RcaI 3149 TCATGA T^CATGA
3489 TCATGA
SapI 895 GAAGAGC GCTCTTC(1/4)
1725 GAAGAGC
SspI 1005 AATATT AAT^ATT
4436 AATATT
TaqI 3155 TCGA T^CGA
4433 TCGA
Tth111I 2688 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5031 GACTCCGTC
TthHB8I 3155 TCGA T^CGA
4433 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AccIII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
ApaLI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaHI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspEI BsrI BssKI
BssSI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII DraIII EaeI EarI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII Hin1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII