################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII AccII AccIII AclI AflII AgeI AhdI AscI AseI AsnI AspEI AviII BciVI BfrI BsaWI BseAI BsiEI BsiWI BsmI BspDI BspEI BsrDI BssHII Bst1107I BstBI BstUI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FseI FspI HgaI HindIII KspI MfeI MluI MroI MunI MvnI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AccI 2595 GTCTAC GT^[AC][GT]AC AflIII 579 ACATGT A^C[GA][CT]GT Alw44I 4448 GTGCAC G^TGCAC Aor51HI 3590 AGCGCT AGC^GCT ApaLI 4448 GTGCAC G^TGCAC Asp700I 4658 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbrPI 2522 CACGTG CAC^GTG BbsI 4816 GTCTTC GAAGAC(2/6) BclI 4379 TGATCA T^GATCA BpuAI 4816 GTCTTC GAAGAC(2/6) BsaAI 2522 CACGTG [CT]AC^GT[GA] BsaBI 4464 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 3195 GAGACG CGTCTC(1/5) Bsp1407I 4908 TGTACA T^GTACA BspHI 4071 TCATGA T^CATGA BspLU11I 579 ACATGT A^CATGT BsrGI 4908 TGTACA T^GTACA DraI 3781 TTTAAA TTT^AAA EarI 1666 CTCTTC CTCTTC(1/4) Eco47III 3590 AGCGCT AGC^GCT FbaI 4379 TGATCA T^GATCA HpaI 4914 GTTAAC GTT^AAC Ksp632I 1666 CTCTTC CTCTTC(1/4) MamI 4464 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PmaCI 2522 CACGTG CAC^GTG PmlI 2522 CACGTG CAC^GTG RcaI 4071 TCATGA T^CATGA SspBI 4908 TGTACA T^GTACA XbaI 2944 TCTAGA T^CTAGA XmnI 4658 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 95 GGTACC G^GTACC 453 GGTACC AcyI 3153 GGCGCC G[GA]^CG[CT]C 3983 GGCGCC ApaI 928 GGGCCC GGGCC^C 1120 GGGCCC Asp718I 95 GGTACC G^GTACC 453 GGTACC AspI 3031 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3340 GACAGAGTC AvrII 439 CCTAGG C^CTAGG 1863 CCTAGG BbeI 3153 GGCGCC GGCGC^C 3983 GGCGCC BcgI 3422 CGATTTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5171 GCAGGAAAATCG BglII 2333 AGATCT A^GATCT 2560 AGATCT BlnI 439 CCTAGG C^CTAGG 1863 CCTAGG BsaHI 3153 GGCGCC G[GA]^CG[CT]C 3983 GGCGCC BssSI 829 CACGAG CACGAG(-5/-1) 3242 CTCGTG BstEII 948 GGTGACC G^GT[ACGT]ACC 2655 GGTTACC BstPI 948 GGTGACC G^GT[ACGT]ACC 2655 GGTTACC EcoNI 1564 CCTACACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4667 CCTTTCCCAGG EcoO65I 948 GGTGACC G^GT[ACGT]ACC 2655 GGTTACC EcoRI 2182 GAATTC G^AATTC 4662 GAATTC Hin1I 3153 GGCGCC G[GA]^CG[CT]C 3983 GGCGCC HincII 1773 GTTGAC GT[CT]^[GA]AC 4914 GTTAAC HindII 1773 GTTGAC GT[CT]^[GA]AC 4914 GTTAAC KasI 3153 GGCGCC G^GCGCC 3983 GGCGCC KpnI 95 GGTACC GGTAC^C 453 GGTACC NarI 3153 GGCGCC GG^CGCC 3983 GGCGCC NcoI 457 CCATGG C^CATGG 641 CCATGG NspI 579 ACATGT [GA]CATG^[CT] 4348 ACATGC PflFI 3031 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3340 GACAGAGTC SacI 1112 GAGCTC GAGCT^C 4271 GAGCTC SfoI 3153 GGCGCC GGC^GCC 3983 GGCGCC SmaI 907 CCCGGG CCC^GGG 1123 CCCGGG SspI 3824 AATATT AAT^ATT 4689 AATATT StuI 2318 AGGCCT AGG^CCT 2407 AGGCCT TfiI 1351 GATTC G^A[AT]TC 2935 GATTC Tth111I 3031 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3340 GACAGAGTC XmaCI 907 CCCGGG C^CCGGG 1123 CCCGGG XmaI 907 CCCGGG C^CCGGG 1123 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BalI BamHI BanI BanII BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII