##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AhdI AscI AseI AsnI
AspEI AviII BciVI BfrI BsaWI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BsrDI BssHII Bst1107I BstBI
BstUI BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoRV
EcoT22I FseI FspI HgaI HindIII
KspI MfeI MluI MroI MunI
MvnI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccI 2595 GTCTAC GT^[AC][GT]AC
AflIII 579 ACATGT A^C[GA][CT]GT
Alw44I 4448 GTGCAC G^TGCAC
Aor51HI 3590 AGCGCT AGC^GCT
ApaLI 4448 GTGCAC G^TGCAC
Asp700I 4658 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 2522 CACGTG CAC^GTG
BbsI 4816 GTCTTC GAAGAC(2/6)
BclI 4379 TGATCA T^GATCA
BpuAI 4816 GTCTTC GAAGAC(2/6)
BsaAI 2522 CACGTG [CT]AC^GT[GA]
BsaBI 4464 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 3195 GAGACG CGTCTC(1/5)
Bsp1407I 4908 TGTACA T^GTACA
BspHI 4071 TCATGA T^CATGA
BspLU11I 579 ACATGT A^CATGT
BsrGI 4908 TGTACA T^GTACA
DraI 3781 TTTAAA TTT^AAA
EarI 1666 CTCTTC CTCTTC(1/4)
Eco47III 3590 AGCGCT AGC^GCT
FbaI 4379 TGATCA T^GATCA
HpaI 4914 GTTAAC GTT^AAC
Ksp632I 1666 CTCTTC CTCTTC(1/4)
MamI 4464 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PmaCI 2522 CACGTG CAC^GTG
PmlI 2522 CACGTG CAC^GTG
RcaI 4071 TCATGA T^CATGA
SspBI 4908 TGTACA T^GTACA
XbaI 2944 TCTAGA T^CTAGA
XmnI 4658 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 95 GGTACC G^GTACC
453 GGTACC
AcyI 3153 GGCGCC G[GA]^CG[CT]C
3983 GGCGCC
ApaI 928 GGGCCC GGGCC^C
1120 GGGCCC
Asp718I 95 GGTACC G^GTACC
453 GGTACC
AspI 3031 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3340 GACAGAGTC
AvrII 439 CCTAGG C^CTAGG
1863 CCTAGG
BbeI 3153 GGCGCC GGCGC^C
3983 GGCGCC
BcgI 3422 CGATTTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5171 GCAGGAAAATCG
BglII 2333 AGATCT A^GATCT
2560 AGATCT
BlnI 439 CCTAGG C^CTAGG
1863 CCTAGG
BsaHI 3153 GGCGCC G[GA]^CG[CT]C
3983 GGCGCC
BssSI 829 CACGAG CACGAG(-5/-1)
3242 CTCGTG
BstEII 948 GGTGACC G^GT[ACGT]ACC
2655 GGTTACC
BstPI 948 GGTGACC G^GT[ACGT]ACC
2655 GGTTACC
EcoNI 1564 CCTACACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4667 CCTTTCCCAGG
EcoO65I 948 GGTGACC G^GT[ACGT]ACC
2655 GGTTACC
EcoRI 2182 GAATTC G^AATTC
4662 GAATTC
Hin1I 3153 GGCGCC G[GA]^CG[CT]C
3983 GGCGCC
HincII 1773 GTTGAC GT[CT]^[GA]AC
4914 GTTAAC
HindII 1773 GTTGAC GT[CT]^[GA]AC
4914 GTTAAC
KasI 3153 GGCGCC G^GCGCC
3983 GGCGCC
KpnI 95 GGTACC GGTAC^C
453 GGTACC
NarI 3153 GGCGCC GG^CGCC
3983 GGCGCC
NcoI 457 CCATGG C^CATGG
641 CCATGG
NspI 579 ACATGT [GA]CATG^[CT]
4348 ACATGC
PflFI 3031 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3340 GACAGAGTC
SacI 1112 GAGCTC GAGCT^C
4271 GAGCTC
SfoI 3153 GGCGCC GGC^GCC
3983 GGCGCC
SmaI 907 CCCGGG CCC^GGG
1123 CCCGGG
SspI 3824 AATATT AAT^ATT
4689 AATATT
StuI 2318 AGGCCT AGG^CCT
2407 AGGCCT
TfiI 1351 GATTC G^A[AT]TC
2935 GATTC
Tth111I 3031 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3340 GACAGAGTC
XmaCI 907 CCCGGG C^CCGGG
1123 CCCGGG
XmaI 907 CCCGGG C^CCGGG
1123 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII