##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AclI AcyI AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AspEI AvaI AviII AvrII
BbeI BbrPI BcgI BciVI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsiEI BsiWI BsmBI BsoBI
BspDI BsrBI BssHII BstBI BstXI
Bsu36I CelII CfoI ClaI CpoI
DraII DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoRI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I KasI KspI MamI
MluI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspV PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SwaI Van91I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccII 1717 CGCG CG^CG
AccIII 1851 TCCGGA T^CCGGA
AspHI 4792 GAGCTC G[AT]GC[AT]^C
AspI 3758 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3393 TGGCCA TGG^CCA
BbvCI 2191 GCTGAGG CCTCAGC(-5/-2)
BsaWI 1851 TCCGGA [AT]^CCGG[AT]
BseAI 1851 TCCGGA T^CCGGA
BsiHKAI 4792 GAGCTC G[AT]GC[AT]^C
BsmFI 380 GTCCC GGGAC(10/14)
BspEI 1851 TCCGGA T^CCGGA
BspHI 4533 TCATGA T^CATGA
BsrFI 3929 ACCGGC [GA]^CCGG[CT]
BssSI 909 CACGAG CACGAG(-5/-1)
Bst1107I 2930 GTATAC GTA^TAC
BstEII 3925 GGTTACC G^GT[ACGT]ACC
BstPI 3925 GGTTACC G^GT[ACGT]ACC
BstUI 1717 CGCG CG^CG
BstZ17I 2930 GTATAC GTA^TAC
Cfr10I 3929 ACCGGC [GA]^CCGG[CT]
DrdI 460 GACTTGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 3393 TGGCCA [CT]^GGCC[GA]
EcoO65I 3925 GGTTACC G^GT[ACGT]ACC
FauI 4 CCCGC CCCGC(4/6)
MluNI 3393 TGGCCA TGG^CCA
MroI 1851 TCCGGA T^CCGGA
MscI 3393 TGGCCA TGG^CCA
MvnI 1717 CGCG CG^CG
PacI 4926 TTAATTAA TTAAT^TAA
PflFI 3758 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 5167 CAGCTG CAG^CTG
RcaI 4533 TCATGA T^CATGA
SacI 4792 GAGCTC GAGCT^C
SfiI 1068 GGCCAAACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 4516 ACTAGT A^CTAGT
StuI 1076 AGGCCT AGG^CCT
Tth111I 3758 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 16 GGTACC G^GTACC
2842 GGTACC
AccI 2930 GTATAC GT^[AC][GT]AC
4091 GTCTAC
AciI 5 CCGC CCGC(-3/-1)
2574 CCGC
AseI 3307 ATTAAT AT^TAAT
6023 ATTAAT
AsnI 3307 ATTAAT AT^TAAT
6023 ATTAAT
Asp718I 16 GGTACC G^GTACC
2842 GGTACC
BamHI 2340 GGATCC G^GATCC
6068 GGATCC
BanII 4792 GAGCTC G[GA]GC[CT]^C
5207 GGGCTC
BbsI 154 GAAGAC GAAGAC(2/6)
5514 GAAGAC
BglI 346 GCCCAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1069 GCCAAACAGGC
BglII 975 AGATCT A^GATCT
1455 AGATCT
BpuAI 154 GAAGAC GAAGAC(2/6)
5514 GAAGAC
BsgI 1664 CTGCAC GTGCAG(16/14)
1889 CTGCAC
Bsp1407I 2975 TGTACA T^GTACA
4039 TGTACA
BsrGI 2975 TGTACA T^GTACA
4039 TGTACA
BstAPI 25 GCAGACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2604 GCATTACATGC
EcoRV 82 GATATC GAT^ATC
1473 GATATC
EcoT22I 454 ATGCAT ATGCA^T
6044 ATGCAT
HpaI 5052 GTTAAC GTT^AAC
5725 GTTAAC
KpnI 16 GGTACC GGTAC^C
2842 GGTACC
MspA1I 5 CCGCTG C[AC]G^C[GT]G
5167 CAGCTG
NsiI 454 ATGCAT ATGCA^T
6044 ATGCAT
PleI 1120 GACTC GAGTC(4/5)
2616 GAGTC
PshBI 3307 ATTAAT AT^TAAT
6023 ATTAAT
ScaI 2684 AGTACT AGT^ACT
4943 AGTACT
SspBI 2975 TGTACA T^GTACA
4039 TGTACA
XbaI 3964 TCTAGA T^CTAGA
4494 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AcsI AfaI AflII AflIII AluI
AlwI AlwNI ApoI Asp700I AvaII
BanI BbvI BclI BcnI BfaI
BfrI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmI Bsp1286I
BspLU11I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraIII EarI
EcoNI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspI MunI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI