##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AccI AccIII AflII AgeI
AlwNI AscI AseI AsnI AviII
BamHI BbrPI BbvCI BfrI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BspEI Bst1107I BstBI
BstZ17I CelII CpoI DrdI EcoRI
EcoRV EcoT22I FseI FspI MamI
MluI MroI NotI NruI NsiI
NspV PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PstI PvuI
RsrII SalI SbfI SfiI SfuI
SgrAI SnaBI SphI Sse8387I SwaI
XcmI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 2659 GGTACC G^GTACC
AclI 4710 AACGTT AA^CGTT
AocI 4109 CCTTAGG CC^T[ACGT]AGG
Aor51HI 184 AGCGCT AGC^GCT
Asp700I 3505 GAATCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2659 GGTACC G^GTACC
AspI 4694 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 248 CCTAGG C^CTAGG
BalI 2970 TGGCCA TGG^CCA
BbeI 348 GGCGCC GGCGC^C
BcgI 1389 GCATAAGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 248 CCTAGG C^CTAGG
BsaI 1591 GGTCTC GGTCTC(1/5)
BsmI 5016 GCATTC GAATGC(1/-1)
BspHI 785 TCATGA T^CATGA
BspMI 3252 ACCTGC ACCTGC(4/8)
BssSI 777 CACGAG CACGAG(-5/-1)
BstAPI 1276 GCAACAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1124 GGTCACC G^GT[ACGT]ACC
BstPI 1124 GGTCACC G^GT[ACGT]ACC
BstXI 1915 CCACTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4109 CCTTAGG CC^T[ACGT]AGG
DraIII 861 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 184 AGCGCT AGC^GCT
Eco81I 4109 CCTTAGG CC^T[ACGT]AGG
EcoO65I 1124 GGTCACC G^GT[ACGT]ACC
HpaI 3170 GTTAAC GTT^AAC
KasI 348 GGCGCC G^GCGCC
KpnI 2659 GGTACC GGTAC^C
KspI 3483 CCGCGG CCGC^GG
MfeI 3840 CAATTG C^AATTG
MluNI 2970 TGGCCA TGG^CCA
MscI 2970 TGGCCA TGG^CCA
MunI 3840 CAATTG C^AATTG
NarI 348 GGCGCC GG^CGCC
NdeI 3802 CATATG CA^TATG
PaeR7I 126 CTCGAG C^TCGAG
PflFI 4694 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2225 CCATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 4710 AACGTT AA^CGTT
RcaI 785 TCATGA T^CATGA
SacI 856 GAGCTC GAGCT^C
SacII 3483 CCGCGG CCGC^GG
SapI 167 GAAGAGC GCTCTTC(1/4)
ScaI 588 AGTACT AGT^ACT
SexAI 4695 ACCTGGT A^CC[AT]GGT
SfoI 348 GGCGCC GGC^GCC
SmaI 152 CCCGGG CCC^GGG
SpeI 4846 ACTAGT A^CTAGT
Tth111I 4694 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2225 CCATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 126 CTCGAG C^TCGAG
XmaCI 152 CCCGGG C^CCGGG
XmaI 152 CCCGGG C^CCGGG
XmnI 3505 GAATCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 329 GGCGTC G[GA]^CG[CT]C
348 GGCGCC
AflIII 2582 ACATGT A^C[GA][CT]GT
4308 ACATGT
AhdI 324 GACTCGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3473 GACATTAAGTC
Alw44I 774 GTGCAC G^TGCAC
1672 GTGCAC
ApaLI 774 GTGCAC G^TGCAC
1672 GTGCAC
AspEI 324 GACTCGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3473 GACATTAAGTC
BclI 1611 TGATCA T^GATCA
4652 TGATCA
BglI 304 GCCCGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2301 GCCAGCTTGGC
BglII 2072 AGATCT A^GATCT
2535 AGATCT
BsaHI 329 GGCGTC G[GA]^CG[CT]C
348 GGCGCC
BsiEI 76 CGGCCG CG[GA][CT]^CG
307 CGGCCG
Bsp1407I 2017 TGTACA T^GTACA
4305 TGTACA
BspDI 3507 ATCGAT AT^CGAT
4867 ATCGAT
BspLU11I 2582 ACATGT A^CATGT
4308 ACATGT
BsrGI 2017 TGTACA T^GTACA
4305 TGTACA
ClaI 3507 ATCGAT AT^CGAT
4867 ATCGAT
EagI 76 CGGCCG C^GGCCG
307 CGGCCG
Eam1105I 324 GACTCGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3473 GACATTAAGTC
EclXI 76 CGGCCG C^GGCCG
307 CGGCCG
Eco52I 76 CGGCCG C^GGCCG
307 CGGCCG
FbaI 1611 TGATCA T^GATCA
4652 TGATCA
Hin1I 329 GGCGTC G[GA]^CG[CT]C
348 GGCGCC
HincII 3170 GTTAAC GT[CT]^[GA]AC
4496 GTTGAC
HindII 3170 GTTAAC GT[CT]^[GA]AC
4496 GTTGAC
HindIII 1310 AAGCTT A^AGCTT
2447 AAGCTT
NheI 2702 GCTAGC G^CTAGC
3845 GCTAGC
PacI 3374 TTAATTAA TTAAT^TAA
5168 TTAATTAA
PvuII 2782 CAGCTG CAG^CTG
3887 CAGCTG
SmlI 126 CTCGAG C^T[CT][GA]AG
1648 CTCAAG
SspBI 2017 TGTACA T^GTACA
4305 TGTACA
StuI 4102 AGGCCT AGG^CCT
4930 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApaI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BciVI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrDI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII