##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AgeI AhdI Alw44I ApaLI
AscI Asp700I AspEI BamHI BbrPI
BciVI BsaBI BsiWI BspHI BspLU11I
BsrDI Bst1107I BstAPI BstEII BstPI
BstZ17I DraIII Eam1105I EcoO65I EcoRI
EcoRV EcoT22I FseI MamI MluI
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PstI PvuII RcaI SacI SalI
SbfI SfcI SfiI SgrAI SnaBI
SpeI SphI Sse8387I StuI SwaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 661 GGTACC G^GTACC
AccIII 1086 TCCGGA T^CCGGA
AflII 3572 CTTAAG C^TTAAG
AocI 4302 CCTCAGG CC^T[ACGT]AGG
ApaI 4049 GGGCCC GGGCC^C
Asp718I 661 GGTACC G^GTACC
AspI 4956 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 548 TGGCCA TGG^CCA
BbvCI 4177 CCTCAGC CCTCAGC(-5/-2)
BcgI 1154 GCACAGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4968 TGATCA T^GATCA
BfrI 3572 CTTAAG C^TTAAG
BlpI 1275 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1275 GCTGAGC GC^T[ACGT]AGC
BseAI 1086 TCCGGA T^CCGGA
BsgI 178 GTGCAG GTGCAG(16/14)
BsmBI 510 CGTCTC CGTCTC(1/5)
BspDI 3211 ATCGAT AT^CGAT
BspEI 1086 TCCGGA T^CCGGA
BstBI 2927 TTCGAA TT^CGAA
BstXI 4187 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4302 CCTCAGG CC^T[ACGT]AGG
CelII 1275 GCTGAGC GC^T[ACGT]AGC
ClaI 3211 ATCGAT AT^CGAT
CpoI 1083 CGGTCCG CG^G[AT]CCG
Eco81I 4302 CCTCAGG CC^T[ACGT]AGG
EcoNI 1470 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 4968 TGATCA T^GATCA
HpaI 1385 GTTAAC GTT^AAC
KpnI 661 GGTACC GGTAC^C
MfeI 4654 CAATTG C^AATTG
MluNI 548 TGGCCA TGG^CCA
MroI 1086 TCCGGA T^CCGGA
MscI 548 TGGCCA TGG^CCA
MunI 4654 CAATTG C^AATTG
NcoI 4913 CCATGG C^CATGG
NspI 4818 ACATGC [GA]CATG^[CT]
NspV 2927 TTCGAA TT^CGAA
PflFI 4956 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 1380 CGATCG CGAT^CG
RsrII 1083 CGGTCCG CG^G[AT]CCG
SapI 1449 GCTCTTC GCTCTTC(1/4)
SexAI 4135 ACCAGGT A^CC[AT]GGT
SfuI 2927 TTCGAA TT^CGAA
Tth111I 4956 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 427 CCACCCGCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 328 GTCTAC GT^[AC][GT]AC
3178 GTAGAC
AclI 1744 AACGTT AA^CGTT
3843 AACGTT
AlwNI 2192 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2531 CAGGGCCTG
Aor51HI 500 AGCGCT AGC^GCT
845 AGCGCT
AspHI 1152 GAGCAC G[AT]GC[AT]^C
1690 GAGCAC
AviII 311 TGCGCA TGC^GCA
4799 TGCGCA
AvrII 729 CCTAGG C^CTAGG
4863 CCTAGG
BglII 242 AGATCT A^GATCT
1109 AGATCT
BlnI 729 CCTAGG C^CTAGG
4863 CCTAGG
BsiHKAI 1152 GAGCAC G[AT]GC[AT]^C
1690 GAGCAC
BsmI 1458 GAATGC GAATGC(1/-1)
4539 GAATGC
Bsp1407I 2041 TGTACA T^GTACA
2380 TGTACA
BsrGI 2041 TGTACA T^GTACA
2380 TGTACA
DrdI 2739 GACCAATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2954 GACTGACTTGTC
EagI 419 CGGCCG C^GGCCG
758 CGGCCG
EclXI 419 CGGCCG C^GGCCG
758 CGGCCG
Eco47III 500 AGCGCT AGC^GCT
845 AGCGCT
Eco52I 419 CGGCCG C^GGCCG
758 CGGCCG
FspI 311 TGCGCA TGC^GCA
4799 TGCGCA
NdeI 3465 CATATG CA^TATG
4393 CATATG
PflMI 2753 CCAATATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4217 CCACAACCTGG
Psp1406I 1744 AACGTT AA^CGTT
3843 AACGTT
ScaI 1904 AGTACT AGT^ACT
5052 AGTACT
SspBI 2041 TGTACA T^GTACA
2380 TGTACA
TfiI 1703 GATTC G^A[AT]TC
4511 GAATC
Van91I 2753 CCAATATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4217 CCACAACCTGG
XbaI 2695 TCTAGA T^CTAGA
3034 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AseI
AsnI AvaI AvaII BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsiEI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV PleI PpuMI
PshAI PshBI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfoI SmaI
SmlI SspI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmaCI XmaI