##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccIII AclI AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BbeI BbrPI
BcgI BlnI BsaAI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BssHII Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I FseI HpaI
KasI KpnI KspI MluI MroI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AatII 2255 GACGTC GACGT^C
AccII 2654 CGCG CG^CG
AcyI 2255 GACGTC G[GA]^CG[CT]C
AviII 101 TGCGCA TGC^GCA
BanI 4060 GGTGCC G^G[CT][GA]CC
BciVI 2711 GGATAC GTATCC(6/5)
BclI 2064 TGATCA T^GATCA
BglII 3395 AGATCT A^GATCT
BsaBI 48 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2255 GACGTC G[GA]^CG[CT]C
BsaI 2768 GGTCTC GGTCTC(1/5)
Bsp1407I 1345 TGTACA T^GTACA
BsrGI 1345 TGTACA T^GTACA
BstEII 2583 GGTGACC G^GT[ACGT]ACC
BstPI 2583 GGTGACC G^GT[ACGT]ACC
BstUI 2654 CGCG CG^CG
EcoO65I 2583 GGTGACC G^GT[ACGT]ACC
EcoRV 4588 GATATC GAT^ATC
FbaI 2064 TGATCA T^GATCA
FspI 101 TGCGCA TGC^GCA
HgaI 2154 GACGC GACGC(5/10)
Hin1I 2255 GACGTC G[GA]^CG[CT]C
HindIII 3805 AAGCTT A^AGCTT
MamI 48 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2654 CGCG CG^CG
NdeI 1715 CATATG CA^TATG
PpuMI 2779 AGGACCT [GA]G^G[AT]CC[CT]
PstI 2790 CTGCAG CTGCA^G
SspBI 1345 TGTACA T^GTACA
TaqI 4940 TCGA T^CGA
TthHB8I 4940 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 712 GTAGAC GT^[AC][GT]AC
2013 GTCTAC
ApaI 2761 GGGCCC GGGCC^C
3329 GGGCCC
BalI 2534 TGGCCA TGG^CCA
4609 TGGCCA
BamHI 621 GGATCC G^GATCC
2518 GGATCC
BglI 1120 GCCCTCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3024 GCCAGGGAGGC
BsaWI 3082 TCCGGT [AT]^CCGG[AT]
4633 TCCGGT
BsmI 2365 GAATGC GAATGC(1/-1)
1392 GCATTC
BsrFI 2872 ACCGGC [GA]^CCGG[CT]
3091 GCCGGT
Cfr10I 2872 ACCGGC [GA]^CCGG[CT]
3091 GCCGGT
EcoRI 567 GAATTC G^AATTC
3914 GAATTC
EcoT22I 1671 ATGCAT ATGCA^T
2524 ATGCAT
HincII 2038 GTTGAC GT[CT]^[GA]AC
2467 GTTGAC
HindII 2038 GTTGAC GT[CT]^[GA]AC
2467 GTTGAC
MluNI 2534 TGGCCA TGG^CCA
4609 TGGCCA
MscI 2534 TGGCCA TGG^CCA
4609 TGGCCA
NsiI 1671 ATGCAT ATGCA^T
2524 ATGCAT
ScaI 2983 AGTACT AGT^ACT
3868 AGTACT
StuI 40 AGGCCT AGG^CCT
2246 AGGCCT
XbaI 4867 TCTAGA T^CTAGA
5013 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanII BbsI BbvCI BbvI
BcnI BfaI BfrI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MspA1I MspI MunI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmaI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII XmaCI
XmaI