##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AseI AsnI Asp700I Asp718I BbsI
BclI BfrI BpuAI BseAI BsiWI
BspDI BspEI Bst1107I BstBI BstZ17I
ClaI CpoI DrdI EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HpaI KpnI MfeI MluI MroI
MunI NdeI NotI NruI NsiI
NspV PacI PmeI PshBI Psp1406I
PvuI RsrII SalI SfiI SfuI
SgrAI SnaBI SpeI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AflIII 783 ACATGT A^C[GA][CT]GT
AgeI 3369 ACCGGT A^CCGGT
AhdI 4148 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 2379 GGCGCGCC GG^CGCGCC
AspEI 4148 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3510 TGCGCA TGC^GCA
BciVI 4916 GTATCC GTATCC(6/5)
BsaBI 3937 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3369 ACCGGT [AT]^CCGG[AT]
BsmBI 4774 GAGACG CGTCTC(1/5)
Bsp1407I 4727 TGTACA T^GTACA
BspLU11I 783 ACATGT A^CATGT
BsrGI 4727 TGTACA T^GTACA
BstEII 3983 GGTAACC G^GT[ACGT]ACC
BstPI 3983 GGTAACC G^GT[ACGT]ACC
EagI 5153 CGGCCG C^GGCCG
Eam1105I 4148 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5153 CGGCCG C^GGCCG
Eco52I 5153 CGGCCG C^GGCCG
EcoNI 3541 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3983 GGTAACC G^GT[ACGT]ACC
FspI 3510 TGCGCA TGC^GCA
HindIII 3656 AAGCTT A^AGCTT
MamI 3937 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 2474 CCATGG C^CATGG
PaeR7I 2588 CTCGAG C^TCGAG
PinAI 3369 ACCGGT A^CCGGT
PshAI 589 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1295 GCTCTTC GCTCTTC(1/4)
SbfI 524 CCTGCAGG CCTGCA^GG
SphI 2707 GCATGC GCATG^C
Sse8387I 524 CCTGCAGG CCTGCA^GG
SspBI 4727 TGTACA T^GTACA
SwaI 176 ATTTAAAT ATTT^AAAT
XbaI 352 TCTAGA T^CTAGA
XhoI 2588 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AatII 4153 GACGTC GACGT^C
4534 GACGTC
Alw44I 3058 GTGCAC G^TGCAC
4780 GTGCAC
Aor51HI 2572 AGCGCT AGC^GCT
3152 AGCGCT
ApaLI 3058 GTGCAC G^TGCAC
4780 GTGCAC
AspI 2688 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3094 GACACCGTC
AvrII 1528 CCTAGG C^CTAGG
1951 CCTAGG
BalI 987 TGGCCA TGG^CCA
3789 TGGCCA
BamHI 1532 GGATCC G^GATCC
1955 GGATCC
BbeI 2697 GGCGCC GGCGC^C
3176 GGCGCC
BbrPI 611 CACGTG CAC^GTG
3785 CACGTG
BlnI 1528 CCTAGG C^CTAGG
1951 CCTAGG
BsaAI 611 CACGTG [CT]AC^GT[GA]
3785 CACGTG
BspHI 3248 TCATGA T^CATGA
4260 TCATGA
DraI 84 TTTAAA TTT^AAA
177 TTTAAA
Eco47III 2572 AGCGCT AGC^GCT
3152 AGCGCT
KasI 2697 GGCGCC G^GCGCC
3176 GGCGCC
MluNI 987 TGGCCA TGG^CCA
3789 TGGCCA
MscI 987 TGGCCA TGG^CCA
3789 TGGCCA
NarI 2697 GGCGCC GG^CGCC
3176 GGCGCC
NheI 2347 GCTAGC G^CTAGC
2467 GCTAGC
PflFI 2688 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3094 GACACCGTC
PmaCI 611 CACGTG CAC^GTG
3785 CACGTG
PmlI 611 CACGTG CAC^GTG
3785 CACGTG
RcaI 3248 TCATGA T^CATGA
4260 TCATGA
SacI 2578 GAGCTC GAGCT^C
4447 GAGCTC
SfoI 2697 GGCGCC GGC^GCC
3176 GGCGCC
SspI 101 AATATT AAT^ATT
108 AATATT
StuI 1064 AGGCCT AGG^CCT
3667 AGGCCT
TfiI 1007 GATTC G^A[AT]TC
2613 GAATC
Tth111I 2688 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3094 GACACCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcgI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssHII BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScaI ScrFI
SexAI SfaNI SfcI SmaI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmaCI XmaI