################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AseI AsnI Asp700I Asp718I BbsI BclI BfrI BpuAI BseAI BsiWI BspDI BspEI Bst1107I BstBI BstZ17I ClaI CpoI DrdI EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HpaI KpnI MfeI MluI MroI MunI NdeI NotI NruI NsiI NspV PacI PmeI PshBI Psp1406I PvuI RsrII SalI SfiI SfuI SgrAI SnaBI SpeI XcmI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AflIII 783 ACATGT A^C[GA][CT]GT AgeI 3369 ACCGGT A^CCGGT AhdI 4148 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AscI 2379 GGCGCGCC GG^CGCGCC AspEI 4148 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 3510 TGCGCA TGC^GCA BciVI 4916 GTATCC GTATCC(6/5) BsaBI 3937 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 3369 ACCGGT [AT]^CCGG[AT] BsmBI 4774 GAGACG CGTCTC(1/5) Bsp1407I 4727 TGTACA T^GTACA BspLU11I 783 ACATGT A^CATGT BsrGI 4727 TGTACA T^GTACA BstEII 3983 GGTAACC G^GT[ACGT]ACC BstPI 3983 GGTAACC G^GT[ACGT]ACC EagI 5153 CGGCCG C^GGCCG Eam1105I 4148 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 5153 CGGCCG C^GGCCG Eco52I 5153 CGGCCG C^GGCCG EcoNI 3541 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 3983 GGTAACC G^GT[ACGT]ACC FspI 3510 TGCGCA TGC^GCA HindIII 3656 AAGCTT A^AGCTT MamI 3937 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 2474 CCATGG C^CATGG PaeR7I 2588 CTCGAG C^TCGAG PinAI 3369 ACCGGT A^CCGGT PshAI 589 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 1295 GCTCTTC GCTCTTC(1/4) SbfI 524 CCTGCAGG CCTGCA^GG SphI 2707 GCATGC GCATG^C Sse8387I 524 CCTGCAGG CCTGCA^GG SspBI 4727 TGTACA T^GTACA SwaI 176 ATTTAAAT ATTT^AAAT XbaI 352 TCTAGA T^CTAGA XhoI 2588 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AatII 4153 GACGTC GACGT^C 4534 GACGTC Alw44I 3058 GTGCAC G^TGCAC 4780 GTGCAC Aor51HI 2572 AGCGCT AGC^GCT 3152 AGCGCT ApaLI 3058 GTGCAC G^TGCAC 4780 GTGCAC AspI 2688 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3094 GACACCGTC AvrII 1528 CCTAGG C^CTAGG 1951 CCTAGG BalI 987 TGGCCA TGG^CCA 3789 TGGCCA BamHI 1532 GGATCC G^GATCC 1955 GGATCC BbeI 2697 GGCGCC GGCGC^C 3176 GGCGCC BbrPI 611 CACGTG CAC^GTG 3785 CACGTG BlnI 1528 CCTAGG C^CTAGG 1951 CCTAGG BsaAI 611 CACGTG [CT]AC^GT[GA] 3785 CACGTG BspHI 3248 TCATGA T^CATGA 4260 TCATGA DraI 84 TTTAAA TTT^AAA 177 TTTAAA Eco47III 2572 AGCGCT AGC^GCT 3152 AGCGCT KasI 2697 GGCGCC G^GCGCC 3176 GGCGCC MluNI 987 TGGCCA TGG^CCA 3789 TGGCCA MscI 987 TGGCCA TGG^CCA 3789 TGGCCA NarI 2697 GGCGCC GG^CGCC 3176 GGCGCC NheI 2347 GCTAGC G^CTAGC 2467 GCTAGC PflFI 2688 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3094 GACACCGTC PmaCI 611 CACGTG CAC^GTG 3785 CACGTG PmlI 611 CACGTG CAC^GTG 3785 CACGTG RcaI 3248 TCATGA T^CATGA 4260 TCATGA SacI 2578 GAGCTC GAGCT^C 4447 GAGCTC SfoI 2697 GGCGCC GGC^GCC 3176 GGCGCC SspI 101 AATATT AAT^ATT 108 AATATT StuI 1064 AGGCCT AGG^CCT 3667 AGGCCT TfiI 1007 GATTC G^A[AT]TC 2613 GAATC Tth111I 2688 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3094 GACACCGTC ######################################### # 3 OR MORE CUT ENZYMES ( 137 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BanI BanII BbvCI BbvI BcgI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssHII BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScaI ScrFI SexAI SfaNI SfcI SmaI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI