##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AvaI AvrII
BanII BbeI BbrPI BbvCI BcgI
BglI BlnI BsaBI BsiHKAI BsiWI
BsoBI BsrBI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
CpoI DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI FauI FseI HaeII KasI
KpnI KspI MamI MfeI MluI
MunI NaeI NarI NcoI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
StuI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 3639 TCCGGA T^CCGGA
AcyI 1294 GACGCC G[GA]^CG[CT]C
AflII 2143 CTTAAG C^TTAAG
AlwNI 83 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 4631 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3627 TGCGCA TGC^GCA
BamHI 4055 GGATCC G^GATCC
BfrI 2143 CTTAAG C^TTAAG
BglII 5305 AGATCT A^GATCT
BlpI 2882 GCTCAGC GC^T[ACGT]AGC
BmyI 2339 GGGCAC G[AGT]GC[ACT]^C
Bpu1102I 2882 GCTCAGC GC^T[ACGT]AGC
BsaHI 1294 GACGCC G[GA]^CG[CT]C
BseAI 3639 TCCGGA T^CCGGA
BsiEI 826 CGATCG CG[GA][CT]^CG
Bsp1286I 2339 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 3645 TGTACA T^GTACA
BspEI 3639 TCCGGA T^CCGGA
BspLU11I 5463 ACATGT A^CATGT
BspMI 4412 GCAGGT ACCTGC(4/8)
BsrGI 3645 TGTACA T^GTACA
CelII 2882 GCTCAGC GC^T[ACGT]AGC
DrdI 3329 GACAACATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 3627 TGCGCA TGC^GCA
Hin1I 1294 GACGCC G[GA]^CG[CT]C
HindIII 5069 AAGCTT A^AGCTT
MroI 3639 TCCGGA T^CCGGA
NheI 5437 GCTAGC G^CTAGC
PflFI 4631 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 5220 CCAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 826 CGATCG CGAT^CG
ScaI 2848 AGTACT AGT^ACT
SpeI 178 ACTAGT A^CTAGT
SphI 1394 GCATGC GCATG^C
SspBI 3645 TGTACA T^GTACA
Tth111I 4631 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 5220 CCAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 787 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AocI 256 CCTGAGG CC^T[ACGT]AGG
2507 CCTCAGG
BciVI 898 GGATAC GTATCC(6/5)
3121 GGATAC
BcnI 2337 CCGGG CC^[GC]GG
4769 CCCGG
BsaWI 1472 TCCGGT [AT]^CCGG[AT]
3639 TCCGGA
BseRI 5571 CTCCTC GAGGAG(10/8)
3677 GAGGAG
BsmBI 4629 GAGACG CGTCTC(1/5)
4799 GAGACG
BsmFI 4744 GGGAC GGGAC(10/14)
5171 GTCCC
BspDI 824 ATCGAT AT^CGAT
828 ATCGAT
BspHI 3151 TCATGA T^CATGA
5575 TCATGA
BsrDI 2430 GCAATG GCAATG(2/0)
3939 GCAATG
BssSI 4083 CTCGTG CACGAG(-5/-1)
4846 CTCGTG
BstAPI 2807 GCACTTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4011 GCAGGGCTTGC
Bsu36I 256 CCTGAGG CC^T[ACGT]AGG
2507 CCTCAGG
ClaI 824 ATCGAT AT^CGAT
828 ATCGAT
DraI 14 TTTAAA TTT^AAA
427 TTTAAA
DraII 513 AGGTCCT [GA]G^G[ACGT]CC[CT]
5332 AGGACCC
Eco81I 256 CCTGAGG CC^T[ACGT]AGG
2507 CCTCAGG
EcoO109I 513 AGGTCCT [GA]G^G[ACGT]CC[CT]
5332 AGGACCC
EcoRV 1078 GATATC GAT^ATC
3020 GATATC
HgaI 1294 GACGC GACGC(5/10)
3209 GCGTC
MspA1I 1412 CAGCTG C[AC]G^C[GT]G
3305 CAGCTG
NciI 2337 CCGGG CC^[GC]GG
4769 CCCGG
PpuMI 513 AGGTCCT [GA]G^G[AT]CC[CT]
5332 AGGACCC
PstI 3384 CTGCAG CTGCA^G
3706 CTGCAG
PvuII 1412 CAGCTG CAG^CTG
3305 CAGCTG
RcaI 3151 TCATGA T^CATGA
5575 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI AseI
AsnI Asp700I AvaII BalI BanI
BbsI BbvI BclI BfaI BpmI
BpuAI BsaAI BsaI BsaJI BsgI
BsiYI BslI BsmAI BsmI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EarI EcoRII EcoT14I
EcoT22I FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NdeI NdeII NlaIII NlaIV
NsiI NspI PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI