################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AclI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AvaI AvrII BanII BbeI BbrPI BbvCI BcgI BglI BlnI BsaBI BsiHKAI BsiWI BsoBI BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I CpoI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI FauI FseI HaeII KasI KpnI KspI MamI MfeI MluI MunI NaeI NarI NcoI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I StuI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccIII 3639 TCCGGA T^CCGGA AcyI 1294 GACGCC G[GA]^CG[CT]C AflII 2143 CTTAAG C^TTAAG AlwNI 83 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG AspI 4631 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 3627 TGCGCA TGC^GCA BamHI 4055 GGATCC G^GATCC BfrI 2143 CTTAAG C^TTAAG BglII 5305 AGATCT A^GATCT BlpI 2882 GCTCAGC GC^T[ACGT]AGC BmyI 2339 GGGCAC G[AGT]GC[ACT]^C Bpu1102I 2882 GCTCAGC GC^T[ACGT]AGC BsaHI 1294 GACGCC G[GA]^CG[CT]C BseAI 3639 TCCGGA T^CCGGA BsiEI 826 CGATCG CG[GA][CT]^CG Bsp1286I 2339 GGGCAC G[AGT]GC[ACT]^C Bsp1407I 3645 TGTACA T^GTACA BspEI 3639 TCCGGA T^CCGGA BspLU11I 5463 ACATGT A^CATGT BspMI 4412 GCAGGT ACCTGC(4/8) BsrGI 3645 TGTACA T^GTACA CelII 2882 GCTCAGC GC^T[ACGT]AGC DrdI 3329 GACAACATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FspI 3627 TGCGCA TGC^GCA Hin1I 1294 GACGCC G[GA]^CG[CT]C HindIII 5069 AAGCTT A^AGCTT MroI 3639 TCCGGA T^CCGGA NheI 5437 GCTAGC G^CTAGC PflFI 4631 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 5220 CCAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuI 826 CGATCG CGAT^CG ScaI 2848 AGTACT AGT^ACT SpeI 178 ACTAGT A^CTAGT SphI 1394 GCATGC GCATG^C SspBI 3645 TGTACA T^GTACA Tth111I 4631 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 5220 CCAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 787 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AocI 256 CCTGAGG CC^T[ACGT]AGG 2507 CCTCAGG BciVI 898 GGATAC GTATCC(6/5) 3121 GGATAC BcnI 2337 CCGGG CC^[GC]GG 4769 CCCGG BsaWI 1472 TCCGGT [AT]^CCGG[AT] 3639 TCCGGA BseRI 5571 CTCCTC GAGGAG(10/8) 3677 GAGGAG BsmBI 4629 GAGACG CGTCTC(1/5) 4799 GAGACG BsmFI 4744 GGGAC GGGAC(10/14) 5171 GTCCC BspDI 824 ATCGAT AT^CGAT 828 ATCGAT BspHI 3151 TCATGA T^CATGA 5575 TCATGA BsrDI 2430 GCAATG GCAATG(2/0) 3939 GCAATG BssSI 4083 CTCGTG CACGAG(-5/-1) 4846 CTCGTG BstAPI 2807 GCACTTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4011 GCAGGGCTTGC Bsu36I 256 CCTGAGG CC^T[ACGT]AGG 2507 CCTCAGG ClaI 824 ATCGAT AT^CGAT 828 ATCGAT DraI 14 TTTAAA TTT^AAA 427 TTTAAA DraII 513 AGGTCCT [GA]G^G[ACGT]CC[CT] 5332 AGGACCC Eco81I 256 CCTGAGG CC^T[ACGT]AGG 2507 CCTCAGG EcoO109I 513 AGGTCCT [GA]G^G[ACGT]CC[CT] 5332 AGGACCC EcoRV 1078 GATATC GAT^ATC 3020 GATATC HgaI 1294 GACGC GACGC(5/10) 3209 GCGTC MspA1I 1412 CAGCTG C[AC]G^C[GT]G 3305 CAGCTG NciI 2337 CCGGG CC^[GC]GG 4769 CCCGG PpuMI 513 AGGTCCT [GA]G^G[AT]CC[CT] 5332 AGGACCC PstI 3384 CTGCAG CTGCA^G 3706 CTGCAG PvuII 1412 CAGCTG CAG^CTG 3305 CAGCTG RcaI 3151 TCATGA T^CATGA 5575 TCATGA ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI ApoI AseI AsnI Asp700I AvaII BalI BanI BbsI BbvI BclI BfaI BpmI BpuAI BsaAI BsaI BsaJI BsgI BsiYI BslI BsmAI BsmI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I EcoT22I FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MvnI MwoI NdeI NdeII NlaIII NlaIV NsiI NspI PleI PshBI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI