##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspEI BbeI BcgI BglII
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrBI BssHII BssSI
BstBI BstEII BstPI ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV HindIII HpaI KasI KpnI
MluI MroI NarI NcoI NheI
NotI NruI NspV PaeR7I PinAI
PmeI PshAI PvuI RsrII SalI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI StuI SwaI XbaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AcyI 5520 GACGCC G[GA]^CG[CT]C
AviII 881 TGCGCA TGC^GCA
AvrII 2097 CCTAGG C^CTAGG
BalI 5456 TGGCCA TGG^CCA
BamHI 903 GGATCC G^GATCC
BbrPI 4809 CACGTG CAC^GTG
BglI 5693 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2097 CCTAGG C^CTAGG
BlpI 1318 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1318 GCTAAGC GC^T[ACGT]AGC
BsaHI 5520 GACGCC G[GA]^CG[CT]C
BsaI 4738 GAGACC GGTCTC(1/5)
BspHI 5327 TCATGA T^CATGA
BsrFI 78 GCCGGC [GA]^CCGG[CT]
Bst1107I 2244 GTATAC GTA^TAC
BstAPI 4155 GCATTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2244 GTATAC GTA^TAC
CelII 1318 GCTAAGC GC^T[ACGT]AGC
Cfr10I 78 GCCGGC [GA]^CCGG[CT]
DsaI 13 CCGCGG C^C[GA][CT]GG
FseI 77 GGCCGGCC GGCCGG^CC
FspI 881 TGCGCA TGC^GCA
HaeII 106 GGCGCT [GA]GCGC^[CT]
Hin1I 5520 GACGCC G[GA]^CG[CT]C
HincII 1178 GTTGAC GT[CT]^[GA]AC
HindII 1178 GTTGAC GT[CT]^[GA]AC
KspI 13 CCGCGG CCGC^GG
MfeI 4228 CAATTG C^AATTG
MluNI 5456 TGGCCA TGG^CCA
MscI 5456 TGGCCA TGG^CCA
MunI 4228 CAATTG C^AATTG
NaeI 78 GCCGGC GCC^GGC
NgoMIV 78 GCCGGC G^CCGGC
PacI 2360 TTAATTAA TTAAT^TAA
PflMI 4805 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4809 CACGTG CAC^GTG
PmlI 4809 CACGTG CAC^GTG
PvuII 5534 CAGCTG CAG^CTG
RcaI 5327 TCATGA T^CATGA
SacII 13 CCGCGG CCGC^GG
SapI 320 GCTCTTC GCTCTTC(1/4)
SbfI 3523 CCTGCAGG CCTGCA^GG
SexAI 41 ACCTGGT A^CC[AT]GGT
SmaI 5572 CCCGGG CCC^GGG
Sse8387I 3523 CCTGCAGG CCTGCA^GG
TaqI 2300 TCGA T^CGA
TthHB8I 2300 TCGA T^CGA
Van91I 4805 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 5572 CCCGGG C^CCGGG
XmaI 5572 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AclI 1073 AACGTT AA^CGTT
5758 AACGTT
AflII 1016 CTTAAG C^TTAAG
3112 CTTAAG
AlwNI 326 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
5703 CAGGTACTG
AspI 40 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5639 GACTCTGTC
AvaI 5540 CTCGGG C^[CT]CG[GA]G
5572 CCCGGG
BanI 151 GGCACC G^G[CT][GA]CC
5729 GGCACC
BbsI 1087 GAAGAC GAAGAC(2/6)
46 GTCTTC
BclI 926 TGATCA T^GATCA
2972 TGATCA
BfrI 1016 CTTAAG C^TTAAG
3112 CTTAAG
BpuAI 1087 GAAGAC GAAGAC(2/6)
46 GTCTTC
BsaAI 3122 CACGTA [CT]AC^GT[GA]
4809 CACGTG
BsaBI 2020 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2296 GATGTCGATC
BsmI 626 GAATGC GAATGC(1/-1)
2830 GCATTC
BsoBI 5540 CTCGGG C^[CT]CG[GA]G
5572 CCCGGG
BspLU11I 894 ACATGT A^CATGT
4147 ACATGT
BsrDI 2396 CATTGC GCAATG(2/0)
1278 GCAATG
BstXI 1972 CCAGCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5396 CCAGTACTTTGG
BstYI 903 GGATCC [GA]^GATC[CT]
3772 AGATCC
FbaI 926 TGATCA T^GATCA
2972 TGATCA
HgaI 5520 GACGC GACGC(5/10)
9 GCGTC
MamI 2020 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2296 GATGTCGATC
MflI 903 GGATCC [GA]^GATC[CT]
3772 AGATCC
MspA1I 13 CCGCGG C[AC]G^C[GT]G
5534 CAGCTG
NdeI 3300 CATATG CA^TATG
4567 CATATG
PflFI 40 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5639 GACTCTGTC
Psp1406I 1073 AACGTT AA^CGTT
5758 AACGTT
ScaI 3116 AGTACT AGT^ACT
5398 AGTACT
Tth111I 40 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5639 GACTCTGTC
XhoII 903 GGATCC [GA]^GATC[CT]
3772 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AocI ApoI
AseI AsnI Asp700I AspHI AvaII
BanII BbvCI BbvI BciVI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstUI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI PshBI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XmnI