##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI AgeI Aor51HI AviII
AvrII BamHI BbrPI BciVI BglI
BlnI BsiEI BsiWI BspDI BsrBI
BssSI BstBI ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoT22I
FseI FspI HindIII MluI NcoI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacI SalI SapI SbfI SfiI
SfuI SgrAI SphI Sse8387I StuI
SwaI Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
Acc65I 5293 GGTACC G^GTACC
AccI 5433 GTATAC GT^[AC][GT]AC
AccIII 4517 TCCGGA T^CCGGA
AcyI 63 GGCGCC G[GA]^CG[CT]C
AhdI 354 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5461 GTGCAC G^TGCAC
AlwNI 5184 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 4292 GGGCCC GGGCC^C
ApaLI 5461 GTGCAC G^TGCAC
AscI 47 GGCGCGCC GG^CGCGCC
Asp700I 3159 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5293 GGTACC G^GTACC
AspEI 354 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 5461 GTGCAC G[AT]GC[AT]^C
AspI 4942 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 63 GGCGCC GGCGC^C
BbvCI 4509 CCTCAGC CCTCAGC(-5/-2)
BcgI 4498 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2850 AGATCT A^GATCT
BsaAI 3664 TACGTA [CT]AC^GT[GA]
BsaHI 63 GGCGCC G[GA]^CG[CT]C
BsaWI 4517 TCCGGA [AT]^CCGG[AT]
BseAI 4517 TCCGGA T^CCGGA
BsiHKAI 5461 GTGCAC G[AT]GC[AT]^C
BsmBI 4413 GAGACG CGTCTC(1/5)
Bsp1407I 1320 TGTACA T^GTACA
BspEI 4517 TCCGGA T^CCGGA
BspHI 1901 TCATGA T^CATGA
BsrFI 2 GCCGGC [GA]^CCGG[CT]
BsrGI 1320 TGTACA T^GTACA
BssHII 48 GCGCGC G^CGCGC
Bst1107I 5433 GTATAC GTA^TAC
BstEII 2316 GGTTACC G^GT[ACGT]ACC
BstPI 2316 GGTTACC G^GT[ACGT]ACC
BstZ17I 5433 GTATAC GTA^TAC
Cfr10I 2 GCCGGC [GA]^CCGG[CT]
Eam1105I 354 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2316 GGTTACC G^GT[ACGT]ACC
EcoRI 2578 GAATTC G^AATTC
Hin1I 63 GGCGCC G[GA]^CG[CT]C
KasI 63 GGCGCC G^GCGCC
KpnI 5293 GGTACC GGTAC^C
MfeI 2552 CAATTG C^AATTG
MroI 4517 TCCGGA T^CCGGA
MunI 2552 CAATTG C^AATTG
NaeI 2 GCCGGC GCC^GGC
NarI 63 GGCGCC GG^CGCC
NgoMIV 2 GCCGGC G^CCGGC
PflFI 4942 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3810 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 724 CTGCAG CTGCA^G
RcaI 1901 TCATGA T^CATGA
ScaI 4739 AGTACT AGT^ACT
SexAI 4976 ACCTGGT A^CC[AT]GGT
SfoI 63 GGCGCC GGC^GCC
SmaI 248 CCCGGG CCC^GGG
SnaBI 3664 TACGTA TAC^GTA
SspBI 1320 TGTACA T^GTACA
Tth111I 4942 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3083 TCTAGA T^CTAGA
XmaCI 248 CCCGGG C^CCGGG
XmaI 248 CCCGGG C^CCGGG
XmnI 3159 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflII 2016 CTTAAG C^TTAAG
3146 CTTAAG
BbsI 2799 GTCTTC GAAGAC(2/6)
3725 GTCTTC
BclI 890 TGATCA T^GATCA
2169 TGATCA
BfrI 2016 CTTAAG C^TTAAG
3146 CTTAAG
BlpI 149 GCTGAGC GC^T[ACGT]AGC
416 GCTGAGC
Bpu1102I 149 GCTGAGC GC^T[ACGT]AGC
416 GCTGAGC
BpuAI 2799 GTCTTC GAAGAC(2/6)
3725 GTCTTC
BsaBI 867 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1662 GATGTCTATC
BseRI 3439 CTCCTC GAGGAG(10/8)
692 GAGGAG
BsmI 2434 GAATGC GAATGC(1/-1)
5139 GCATTC
BstYI 2778 GGATCT [GA]^GATC[CT]
2850 AGATCT
CelII 149 GCTGAGC GC^T[ACGT]AGC
416 GCTGAGC
DsaI 21 CCGCGG C^C[GA][CT]GG
364 CCGCGG
EcoNI 4917 CCTAGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4977 CCTGGTCTAGG
EcoRV 1259 GATATC GAT^ATC
2332 GATATC
EcoT14I 339 CCTTGG C^C[AT][AT]GG
2838 CCTTGG
FbaI 890 TGATCA T^GATCA
2169 TGATCA
HincII 986 GTTAAC GT[CT]^[GA]AC
1652 GTTAAC
HindII 986 GTTAAC GT[CT]^[GA]AC
1652 GTTAAC
HpaI 986 GTTAAC GTT^AAC
1652 GTTAAC
KspI 21 CCGCGG CCGC^GG
364 CCGCGG
MaeII 1746 ACGT A^CGT
3665 ACGT
MamI 867 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1662 GATGTCTATC
MflI 2778 GGATCT [GA]^GATC[CT]
2850 AGATCT
PvuII 701 CAGCTG CAG^CTG
3760 CAGCTG
SacII 21 CCGCGG CCGC^GG
364 CCGCGG
SfcI 724 CTGCAG C^T[GA][CT]AG
2846 CTATAG
SpeI 1471 ACTAGT A^CTAGT
5361 ACTAGT
StyI 339 CCTTGG C^C[AT][AT]GG
2838 CCTTGG
XhoII 2778 GGATCT [GA]^GATC[CT]
2850 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AocI ApoI AseI
AsnI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DraIII EaeI
EarI Eco81I EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV NspI PleI PpuMI
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I