##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AocI Aor51HI AscI Asp718I
AspI AvaI AviII AvrII BbeI
BbrPI BcgI BclI BcnI BlnI
BlpI Bpu1102I BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BsrBI
BsrFI BssHII BssSI BstAPI BstBI
BstUI Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoT22I
FauI FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MluI MroI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PvuI
PvuII RcaI RsrII SacII SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
StuI Tth111I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AclI 4390 AACGTT AA^CGTT
AhdI 679 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1809 GGGCCC GGGCC^C
AspEI 679 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1672 TGGCCA TGG^CCA
BamHI 3361 GGATCC G^GATCC
BanI 2522 GGTGCC G^G[CT][GA]CC
BbvCI 867 CCTCAGC CCTCAGC(-5/-2)
BglII 3754 AGATCT A^GATCT
BsaAI 4577 CACGTA [CT]AC^GT[GA]
BsaBI 3503 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1954 GAGGAG GAGGAG(10/8)
BsmI 4779 GAATGC GAATGC(1/-1)
BspMI 4414 GCAGGT ACCTGC(4/8)
Bst1107I 765 GTATAC GTA^TAC
BstEII 3867 GGTAACC G^GT[ACGT]ACC
BstPI 3867 GGTAACC G^GT[ACGT]ACC
BstXI 3673 CCAGAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 765 GTATAC GTA^TAC
Eam1105I 679 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3867 GGTAACC G^GT[ACGT]ACC
EcoRI 2168 GAATTC G^AATTC
EcoRV 1167 GATATC GAT^ATC
HgaI 310 GCGTC GACGC(5/10)
HindIII 3416 AAGCTT A^AGCTT
MamI 3503 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1672 TGGCCA TGG^CCA
MscI 1672 TGGCCA TGG^CCA
MspA1I 4711 CAGCGG C[AC]G^C[GT]G
PpuMI 4664 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 4390 AACGTT AA^CGTT
SalI 3666 GTCGAC G^TCGAC
SwaI 2271 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 765 GTATAC GT^[AC][GT]AC
3666 GTCGAC
AflII 1254 CTTAAG C^TTAAG
4463 CTTAAG
AvaII 1959 GGTCC G^G[AT]CC
4665 GGTCC
BciVI 47 GGATAC GTATCC(6/5)
2331 GGATAC
BfrI 1254 CTTAAG C^TTAAG
4463 CTTAAG
BglI 3843 GCCATTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3924 GCCCAATGGGC
BspLU11I 121 ACATGT A^CATGT
4825 ACATGT
BsrDI 4924 CATTGC GCAATG(2/0)
881 GCAATG
BstYI 3361 GGATCC [GA]^GATC[CT]
3754 AGATCT
DraII 1808 AGGGCCC [GA]G^G[ACGT]CC[CT]
4664 AGGTCCC
DrdI 1972 GACATACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2445 GACAAAGAAGTC
EaeI 1672 TGGCCA [CT]^GGCC[GA]
3850 TGGCCG
EcoO109I 1808 AGGGCCC [GA]G^G[ACGT]CC[CT]
4664 AGGTCCC
HapII 314 CCGG C^CGG
3853 CCGG
HpaII 314 CCGG C^CGG
3853 CCGG
MfeI 2788 CAATTG C^AATTG
2814 CAATTG
MflI 3361 GGATCC [GA]^GATC[CT]
3754 AGATCT
MspI 314 CCGG C^CGG
3853 CCGG
MunI 2788 CAATTG C^AATTG
2814 CAATTG
PstI 148 CTGCAG CTGCA^G
4434 CTGCAG
SacI 894 GAGCTC GAGCT^C
2210 GAGCTC
ScaI 3064 AGTACT AGT^ACT
4972 AGTACT
SpeI 1791 ACTAGT A^CTAGT
2092 ACTAGT
XhoII 3361 GGATCC [GA]^GATC[CT]
3754 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI ApaLI ApoI
AseI AsnI Asp700I AspHI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoNI EcoRII EcoT14I Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XmnI