##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspI AviII AvrII BalI
BbrPI BbvCI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsiWI BsmBI
BspDI BspLU11I BsrBI BstBI BstEII
BstPI CelII ClaI CpoI DraIII
Eco47III EcoO65I EcoRI EcoT22I FbaI
FseI FspI HindIII KpnI MluI
MluNI MscI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SalI SbfI SfiI
SfuI SgrAI SnaBI SphI Sse8387I
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 2986 GACGTC GACGT^C
AccIII 2300 TCCGGA T^CCGGA
AhdI 2853 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 2148 ATTAAT AT^TAAT
AsnI 2148 ATTAAT AT^TAAT
Asp700I 2313 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2853 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 2041 GTATCC GTATCC(6/5)
BglII 1025 AGATCT A^GATCT
BseAI 2300 TCCGGA T^CCGGA
BsgI 1394 CTGCAC GTGCAG(16/14)
BsmI 3416 GAATGC GAATGC(1/-1)
BspEI 2300 TCCGGA T^CCGGA
BspMI 290 GCAGGT ACCTGC(4/8)
BssHII 205 GCGCGC G^CGCGC
BstAPI 4857 GCAGAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2736 CCAGTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 117 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 3951 GACAGCTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2853 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 117 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 3530 GATATC GAT^ATC
HgaI 2786 GACGC GACGC(5/10)
MroI 2300 TCCGGA T^CCGGA
PpuMI 117 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 2148 ATTAAT AT^TAAT
PstI 1975 CTGCAG CTGCA^G
SacI 4665 GAGCTC GAGCT^C
SexAI 2952 ACCTGGT A^CC[AT]GGT
SmaI 135 CCCGGG CCC^GGG
SpeI 4652 ACTAGT A^CTAGT
SwaI 4613 ATTTAAAT ATTT^AAAT
XbaI 2255 TCTAGA T^CTAGA
XmaCI 135 CCCGGG C^CCGGG
XmaI 135 CCCGGG C^CCGGG
XmnI 2313 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AlwNI 291 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
4474 CAGTCTCTG
AocI 1818 CCTGAGG CC^T[ACGT]AGG
3499 CCTTAGG
AspHI 4310 GTGCTC G[AT]GC[AT]^C
4665 GAGCTC
BanII 2731 GAGCCC G[GA]GC[CT]^C
4665 GAGCTC
BbeI 245 GGCGCC GGCGC^C
259 GGCGCC
BcgI 1802 CGAGTTCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1922 CGAAGAAGATGC
BglI 60 GCCAGAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3392 GCCCAGCTGGC
BsaBI 2601 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3819 GATTTGCATC
BsiEI 158 CGGCCG CG[GA][CT]^CG
226 CGGCCG
BsiHKAI 4310 GTGCTC G[AT]GC[AT]^C
4665 GAGCTC
BspHI 2576 TCATGA T^CATGA
4974 TCATGA
BsrFI 160 GCCGGC [GA]^CCGG[CT]
228 GCCGGC
BssSI 4760 CACGAG CACGAG(-5/-1)
5134 CACGAG
Bsu36I 1818 CCTGAGG CC^T[ACGT]AGG
3499 CCTTAGG
Cfr10I 160 GCCGGC [GA]^CCGG[CT]
228 GCCGGC
EagI 158 CGGCCG C^GGCCG
226 CGGCCG
EclXI 158 CGGCCG C^GGCCG
226 CGGCCG
Eco52I 158 CGGCCG C^GGCCG
226 CGGCCG
Eco81I 1818 CCTGAGG CC^T[ACGT]AGG
3499 CCTTAGG
HaeII 245 GGCGCC [GA]GCGC^[CT]
259 GGCGCC
HpaI 381 GTTAAC GTT^AAC
1137 GTTAAC
KasI 245 GGCGCC G^GCGCC
259 GGCGCC
KspI 81 CCGCGG CCGC^GG
124 CCGCGG
MamI 2601 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3819 GATTTGCATC
MfeI 538 CAATTG C^AATTG
910 CAATTG
MunI 538 CAATTG C^AATTG
910 CAATTG
NaeI 160 GCCGGC GCC^GGC
228 GCCGGC
NarI 245 GGCGCC GG^CGCC
259 GGCGCC
NdeI 2055 CATATG CA^TATG
4101 CATATG
NgoMIV 160 GCCGGC G^CCGGC
228 GCCGGC
NspI 4185 ACATGC [GA]CATG^[CT]
5065 GCATGT
PflMI 1590 CCATTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2912 CCATGGGGTGG
PshAI 2453 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3978 GACCATGGTC
PvuII 395 CAGCTG CAG^CTG
3395 CAGCTG
RcaI 2576 TCATGA T^CATGA
4974 TCATGA
SacII 81 CCGCGG CCGC^GG
124 CCGCGG
ScaI 3796 AGTACT AGT^ACT
4244 AGTACT
SfoI 245 GGCGCC GGC^GCC
259 GGCGCC
Van91I 1590 CCATTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2912 CCATGGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI ApoI AvaI
AvaII BamHI BanI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BsrDI BsrGI BsrI
BssKI Bst1107I BstF5I BstNI BstUI
BstYI BstZ17I Cac8I CfoI Cfr13I
DdeI DpnII DraI DsaI EaeI
EarI EcoNI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII