##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BciVI BfrI
BlnI BsaBI BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrGI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI Eam1105I EcoRI EcoRV EcoT22I
HpaI KpnI MamI MfeI MroI
MunI NdeI NheI NotI NsiI
NspV PacI PinAI PmeI PshBI
Psp1406I RsrII SalI SbfI SfuI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 16 enzymes) #
###################################
AccI 1579 GTCTAC GT^[AC][GT]AC
AspI 4719 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2423 TGCGCA TGC^GCA
BglII 1391 AGATCT A^GATCT
BsaWI 1596 ACCGGA [AT]^CCGG[AT]
BsmI 5164 GCATTC GAATGC(1/-1)
FspI 2423 TGCGCA TGC^GCA
MluI 3317 ACGCGT A^CGCGT
NruI 4193 TCGCGA TCG^CGA
PflFI 4719 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 4262 CGATCG CGAT^CG
SexAI 733 ACCTGGT A^CC[AT]GGT
SfiI 4552 GGCCAGGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 870 CGCCGGCG C[GA]^CCGG[CT]G
TfiI 2989 GATTC G^A[AT]TC
Tth111I 4719 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AocI 2997 CCTCAGG CC^T[ACGT]AGG
3009 CCTCAGG
BbeI 237 GGCGCC GGCGC^C
2002 GGCGCC
BbrPI 1245 CACGTG CAC^GTG
3799 CACGTG
BclI 2072 TGATCA T^GATCA
3444 TGATCA
Bsu36I 2997 CCTCAGG CC^T[ACGT]AGG
3009 CCTCAGG
Eco81I 2997 CCTCAGG CC^T[ACGT]AGG
3009 CCTCAGG
FbaI 2072 TGATCA T^GATCA
3444 TGATCA
FseI 4322 GGCCGGCC GGCCGG^CC
4697 GGCCGGCC
HincII 451 GTCAAC GT[CT]^[GA]AC
1408 GTCAAC
HindII 451 GTCAAC GT[CT]^[GA]AC
1408 GTCAAC
HindIII 595 AAGCTT A^AGCTT
5363 AAGCTT
KasI 237 GGCGCC G^GCGCC
2002 GGCGCC
NarI 237 GGCGCC GG^CGCC
2002 GGCGCC
PaeR7I 164 CTCGAG C^TCGAG
3985 CTCGAG
PmaCI 1245 CACGTG CAC^GTG
3799 CACGTG
PmlI 1245 CACGTG CAC^GTG
3799 CACGTG
PshAI 3396 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4599 GACGGGCGTC
ScaI 4316 AGTACT AGT^ACT
4349 AGTACT
SfoI 237 GGCGCC GGC^GCC
2002 GGCGCC
SmaI 1198 CCCGGG CCC^GGG
4424 CCCGGG
SphI 3038 GCATGC GCATG^C
4238 GCATGC
XhoI 164 CTCGAG C^TCGAG
3985 CTCGAG
XmaCI 1198 CCCGGG C^CCGGG
4424 CCCGGG
XmaI 1198 CCCGGG C^CCGGG
4424 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI Aor51HI ApaI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcgI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrI
BssHII BssKI BssSI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
SacII SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII