################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BciVI BfrI BlnI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI Eam1105I EcoRI EcoRV EcoT22I HpaI KpnI MamI MfeI MroI MunI NdeI NheI NotI NsiI NspV PacI PinAI PmeI PshBI Psp1406I RsrII SalI SbfI SfuI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 16 enzymes) # ################################### AccI 1579 GTCTAC GT^[AC][GT]AC AspI 4719 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2423 TGCGCA TGC^GCA BglII 1391 AGATCT A^GATCT BsaWI 1596 ACCGGA [AT]^CCGG[AT] BsmI 5164 GCATTC GAATGC(1/-1) FspI 2423 TGCGCA TGC^GCA MluI 3317 ACGCGT A^CGCGT NruI 4193 TCGCGA TCG^CGA PflFI 4719 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuI 4262 CGATCG CGAT^CG SexAI 733 ACCTGGT A^CC[AT]GGT SfiI 4552 GGCCAGGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 870 CGCCGGCG C[GA]^CCGG[CT]G TfiI 2989 GATTC G^A[AT]TC Tth111I 4719 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AocI 2997 CCTCAGG CC^T[ACGT]AGG 3009 CCTCAGG BbeI 237 GGCGCC GGCGC^C 2002 GGCGCC BbrPI 1245 CACGTG CAC^GTG 3799 CACGTG BclI 2072 TGATCA T^GATCA 3444 TGATCA Bsu36I 2997 CCTCAGG CC^T[ACGT]AGG 3009 CCTCAGG Eco81I 2997 CCTCAGG CC^T[ACGT]AGG 3009 CCTCAGG FbaI 2072 TGATCA T^GATCA 3444 TGATCA FseI 4322 GGCCGGCC GGCCGG^CC 4697 GGCCGGCC HincII 451 GTCAAC GT[CT]^[GA]AC 1408 GTCAAC HindII 451 GTCAAC GT[CT]^[GA]AC 1408 GTCAAC HindIII 595 AAGCTT A^AGCTT 5363 AAGCTT KasI 237 GGCGCC G^GCGCC 2002 GGCGCC NarI 237 GGCGCC GG^CGCC 2002 GGCGCC PaeR7I 164 CTCGAG C^TCGAG 3985 CTCGAG PmaCI 1245 CACGTG CAC^GTG 3799 CACGTG PmlI 1245 CACGTG CAC^GTG 3799 CACGTG PshAI 3396 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4599 GACGGGCGTC ScaI 4316 AGTACT AGT^ACT 4349 AGTACT SfoI 237 GGCGCC GGC^GCC 2002 GGCGCC SmaI 1198 CCCGGG CCC^GGG 4424 CCCGGG SphI 3038 GCATGC GCATG^C 4238 GCATGC XhoI 164 CTCGAG C^TCGAG 3985 CTCGAG XmaCI 1198 CCCGGG C^CCGGG 4424 CCCGGG XmaI 1198 CCCGGG C^CCGGG 4424 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 150 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI Aor51HI ApaI AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrI BssHII BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI SacII SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII