##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AccIII AclI AflII AgeI AocI
Aor51HI AscI AspI AviII BamHI
BbeI BbvCI BcgI BcnI BfrI
BglI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BseRI BsiWI BsmBI
BspEI BsrFI BssHII BssSI BstBI
Bsu36I CelII Cfr10I CpoI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV FauI FseI FspI
HaeII HapII HpaII KasI KspI
MamI MfeI MluI MroI MspI
MunI NaeI NarI NciI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
Psp1406I RsrII SacII SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 4534 GGTACC G^GTACC
AccII 1 CGCG CG^CG
ApaI 1226 GGGCCC GGGCC^C
Asp700I 247 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4534 GGTACC G^GTACC
AvaI 3938 CCCGAG C^[CT]CG[GA]G
AvrII 2406 CCTAGG C^CTAGG
BalI 5063 TGGCCA TGG^CCA
BbrPI 1293 CACGTG CAC^GTG
BciVI 3399 GTATCC GTATCC(6/5)
BlnI 2406 CCTAGG C^CTAGG
BpmI 3456 CTCCAG CTGGAG(16/14)
BsaAI 1293 CACGTG [CT]AC^GT[GA]
BsmFI 3803 GTCCC GGGAC(10/14)
BsoBI 3938 CCCGAG C^[CT]CG[GA]G
Bsp1407I 3511 TGTACA T^GTACA
BsrBI 4302 GAGCGG CCGCTC(-3/-3)
BsrDI 2743 CATTGC GCAATG(2/0)
BsrGI 3511 TGTACA T^GTACA
Bst1107I 820 GTATAC GTA^TAC
BstEII 4821 GGTTACC G^GT[ACGT]ACC
BstPI 4821 GGTTACC G^GT[ACGT]ACC
BstUI 1 CGCG CG^CG
BstXI 1439 CCAAAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 820 GTATAC GTA^TAC
CfoI 4060 GCGC GCG^C
DraII 4361 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 4320 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2638 CCATGG C^C[GA][CT]GG
EaeI 5063 TGGCCA [CT]^GGCC[GA]
EcoO109I 4361 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 4821 GGTTACC G^GT[ACGT]ACC
EcoRI 316 GAATTC G^AATTC
HgaI 2 GCGTC GACGC(5/10)
HhaI 4060 GCGC GCG^C
HinP1I 4060 GCGC G^CGC
KpnI 4534 GGTACC GGTAC^C
MluNI 5063 TGGCCA TGG^CCA
MscI 5063 TGGCCA TGG^CCA
MspA1I 4473 CAGCTG C[AC]G^C[GT]G
MvnI 1 CGCG CG^CG
NcoI 2638 CCATGG C^CATGG
NdeI 1741 CATATG CA^TATG
PflMI 1882 CCAACTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1293 CACGTG CAC^GTG
PmlI 1293 CACGTG CAC^GTG
PshAI 374 GACTAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 4602 CTGCAG CTGCA^G
PvuII 4473 CAGCTG CAG^CTG
SacI 4288 GAGCTC GAGCT^C
SalI 3924 GTCGAC G^TCGAC
SapI 2736 GCTCTTC GCTCTTC(1/4)
ScaI 2998 AGTACT AGT^ACT
SspBI 3511 TGTACA T^GTACA
StuI 4748 AGGCCT AGG^CCT
Van91I 1882 CCAACTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4742 TCTAGA T^CTAGA
XmnI 247 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AatII 1847 GACGTC GACGT^C
4185 GACGTC
AcyI 1847 GACGTC G[GA]^CG[CT]C
4185 GACGTC
AvaII 1289 GGTCC G^G[AT]CC
5096 GGTCC
BanI 4534 GGTACC G^G[CT][GA]CC
5167 GGCACC
BanII 1226 GGGCCC G[GA]GC[CT]^C
4288 GAGCTC
BsaHI 1847 GACGTC G[GA]^CG[CT]C
4185 GACGTC
BsgI 985 CTGCAC GTGCAG(16/14)
3786 CTGCAC
BsiEI 4672 CGATCG CG[GA][CT]^CG
4685 CGATCG
BsmI 2443 GCATTC GAATGC(1/-1)
3361 GCATTC
BstAPI 3603 GCATATAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4604 GCAGTTGTTGC
EarI 2737 CTCTTC CTCTTC(1/4)
3132 CTCTTC
EcoT22I 2002 ATGCAT ATGCA^T
4014 ATGCAT
Hin1I 1847 GACGTC G[GA]^CG[CT]C
4185 GACGTC
HpaI 2890 GTTAAC GTT^AAC
4387 GTTAAC
Ksp632I 2737 CTCTTC CTCTTC(1/4)
3132 CTCTTC
NsiI 2002 ATGCAT ATGCA^T
4014 ATGCAT
PvuI 4672 CGATCG CGAT^CG
4685 CGATCG
SpeI 118 ACTAGT A^CTAGT
4064 ACTAGT
XcmI 792 CCATTTGTTAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1439 CCAAAGTAATGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AhdI AluI Alw44I AlwI AlwNI
ApaLI ApoI AseI AsnI AspEI
AspHI BbsI BbvI BclI BfaI
BglII BmyI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BspDI
BspHI BspLU11I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
ClaI DdeI DpnII DraI Eam1105I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HincII HindII HindIII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PshBI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII