##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AspEI
AviII BalI BamHI BbeI BbrPI
BcgI BclI BcnI BglI BlpI
Bpu1102I BsaAI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BspEI
BspHI BsrFI BssHII BssSI BstBI
CelII Cfr10I CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I FbaI FseI FspI HaeII
HapII HindIII HpaII KasI KspI
MluI MluNI MroI MscI MspI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 1478 GGTACC G^GTACC
AclI 1036 AACGTT AA^CGTT
AcyI 1111 GACGCC G[GA]^CG[CT]C
AlwNI 1962 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 5198 CCTTAGG CC^T[ACGT]AGG
Asp700I 297 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1478 GGTACC G^GTACC
AspHI 1560 GAGCTC G[AT]GC[AT]^C
AspI 3976 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 481 CTCGGG C^[CT]CG[GA]G
AvrII 4446 CCTAGG C^CTAGG
BanI 1478 GGTACC G^G[CT][GA]CC
BanII 1560 GAGCTC G[GA]GC[CT]^C
BbsI 1543 GAAGAC GAAGAC(2/6)
BbvCI 5079 CCTCAGC CCTCAGC(-5/-2)
BciVI 3091 GTATCC GTATCC(6/5)
BglII 742 AGATCT A^GATCT
BlnI 4446 CCTAGG C^CTAGG
BpuAI 1543 GAAGAC GAAGAC(2/6)
BsaHI 1111 GACGCC G[GA]^CG[CT]C
BsgI 3036 CTGCAC GTGCAG(16/14)
BsiHKAI 1560 GAGCTC G[AT]GC[AT]^C
BsoBI 481 CTCGGG C^[CT]CG[GA]G
BspDI 4336 ATCGAT AT^CGAT
BsrBI 584 GAGCGG CCGCTC(-3/-3)
BstAPI 1066 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3781 GGTAACC G^GT[ACGT]ACC
BstPI 3781 GGTAACC G^GT[ACGT]ACC
Bsu36I 5198 CCTTAGG CC^T[ACGT]AGG
CfoI 1808 GCGC GCG^C
ClaI 4336 ATCGAT AT^CGAT
DraII 2866 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eco81I 5198 CCTTAGG CC^T[ACGT]AGG
EcoNI 4864 CCTTTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2866 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 3781 GGTAACC G^GT[ACGT]ACC
FauI 586 GCGGG CCCGC(4/6)
HhaI 1808 GCGC GCG^C
Hin1I 1111 GACGCC G[GA]^CG[CT]C
HinP1I 1808 GCGC G^CGC
HpaI 2522 GTTAAC GTT^AAC
KpnI 1478 GGTACC GGTAC^C
PflFI 3976 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3555 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1036 AACGTT AA^CGTT
SacI 1560 GAGCTC GAGCT^C
SapI 213 GCTCTTC GCTCTTC(1/4)
ScaI 5228 AGTACT AGT^ACT
StuI 5065 AGGCCT AGG^CCT
SwaI 4413 ATTTAAAT ATTT^AAAT
Tth111I 3976 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 172 CCAGTCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 297 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 1809 CGCG CG^CG
4748 CGCG
BmyI 1560 GAGCTC G[AGT]GC[ACT]^C
4440 GTGCCC
BsaBI 2827 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3651 GATTCATATC
Bsp1286I 1560 GAGCTC G[AGT]GC[ACT]^C
4440 GTGCCC
BstUI 1809 CGCG CG^CG
4748 CGCG
BstXI 2950 CCAACCATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5061 CCATAGGCCTGG
BstYI 168 AGATCC [GA]^GATC[CT]
742 AGATCT
DsaI 907 CCATGG C^C[GA][CT]GG
3786 CCATGG
EcoRI 3283 GAATTC G^AATTC
4363 GAATTC
EcoRV 1922 GATATC GAT^ATC
2064 GATATC
HincII 2522 GTTAAC GT[CT]^[GA]AC
4584 GTCAAC
HindII 2522 GTTAAC GT[CT]^[GA]AC
4584 GTCAAC
MamI 2827 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3651 GATTCATATC
MfeI 363 CAATTG C^AATTG
4662 CAATTG
MflI 168 AGATCC [GA]^GATC[CT]
742 AGATCT
MspA1I 968 CAGCTG C[AC]G^C[GT]G
5133 CAGCTG
MunI 363 CAATTG C^AATTG
4662 CAATTG
MvnI 1809 CGCG CG^CG
4748 CGCG
NcoI 907 CCATGG C^CATGG
3786 CCATGG
NdeI 2908 CATATG CA^TATG
2955 CATATG
PflMI 329 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1520 CCAGGATTTGG
PvuII 968 CAGCTG CAG^CTG
5133 CAGCTG
Van91I 329 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1520 CCAGGATTTGG
XhoII 168 AGATCC [GA]^GATC[CT]
742 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI ApoI AseI
AsnI AvaII BbvI BfaI BfrI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BsmI Bsp1407I BspLU11I BspMI
BsrDI BsrGI BsrI BssKI Bst1107I
BstF5I BstNI BstZ17I Cac8I Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HgaI HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PleI PshBI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI