################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII AccIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AspEI AviII BalI BamHI BbeI BbrPI BcgI BclI BcnI BglI BlpI Bpu1102I BsaAI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BspEI BspHI BsrFI BssHII BssSI BstBI CelII Cfr10I CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I FbaI FseI FspI HaeII HapII HindIII HpaII KasI KspI MluI MluNI MroI MscI MspI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 1478 GGTACC G^GTACC AclI 1036 AACGTT AA^CGTT AcyI 1111 GACGCC G[GA]^CG[CT]C AlwNI 1962 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 5198 CCTTAGG CC^T[ACGT]AGG Asp700I 297 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1478 GGTACC G^GTACC AspHI 1560 GAGCTC G[AT]GC[AT]^C AspI 3976 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 481 CTCGGG C^[CT]CG[GA]G AvrII 4446 CCTAGG C^CTAGG BanI 1478 GGTACC G^G[CT][GA]CC BanII 1560 GAGCTC G[GA]GC[CT]^C BbsI 1543 GAAGAC GAAGAC(2/6) BbvCI 5079 CCTCAGC CCTCAGC(-5/-2) BciVI 3091 GTATCC GTATCC(6/5) BglII 742 AGATCT A^GATCT BlnI 4446 CCTAGG C^CTAGG BpuAI 1543 GAAGAC GAAGAC(2/6) BsaHI 1111 GACGCC G[GA]^CG[CT]C BsgI 3036 CTGCAC GTGCAG(16/14) BsiHKAI 1560 GAGCTC G[AT]GC[AT]^C BsoBI 481 CTCGGG C^[CT]CG[GA]G BspDI 4336 ATCGAT AT^CGAT BsrBI 584 GAGCGG CCGCTC(-3/-3) BstAPI 1066 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3781 GGTAACC G^GT[ACGT]ACC BstPI 3781 GGTAACC G^GT[ACGT]ACC Bsu36I 5198 CCTTAGG CC^T[ACGT]AGG CfoI 1808 GCGC GCG^C ClaI 4336 ATCGAT AT^CGAT DraII 2866 AGGGCCT [GA]G^G[ACGT]CC[CT] Eco81I 5198 CCTTAGG CC^T[ACGT]AGG EcoNI 4864 CCTTTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 2866 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 3781 GGTAACC G^GT[ACGT]ACC FauI 586 GCGGG CCCGC(4/6) HhaI 1808 GCGC GCG^C Hin1I 1111 GACGCC G[GA]^CG[CT]C HinP1I 1808 GCGC G^CGC HpaI 2522 GTTAAC GTT^AAC KpnI 1478 GGTACC GGTAC^C PflFI 3976 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 3555 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1036 AACGTT AA^CGTT SacI 1560 GAGCTC GAGCT^C SapI 213 GCTCTTC GCTCTTC(1/4) ScaI 5228 AGTACT AGT^ACT StuI 5065 AGGCCT AGG^CCT SwaI 4413 ATTTAAAT ATTT^AAAT Tth111I 3976 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 172 CCAGTCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 297 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 1809 CGCG CG^CG 4748 CGCG BmyI 1560 GAGCTC G[AGT]GC[ACT]^C 4440 GTGCCC BsaBI 2827 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3651 GATTCATATC Bsp1286I 1560 GAGCTC G[AGT]GC[ACT]^C 4440 GTGCCC BstUI 1809 CGCG CG^CG 4748 CGCG BstXI 2950 CCAACCATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5061 CCATAGGCCTGG BstYI 168 AGATCC [GA]^GATC[CT] 742 AGATCT DsaI 907 CCATGG C^C[GA][CT]GG 3786 CCATGG EcoRI 3283 GAATTC G^AATTC 4363 GAATTC EcoRV 1922 GATATC GAT^ATC 2064 GATATC HincII 2522 GTTAAC GT[CT]^[GA]AC 4584 GTCAAC HindII 2522 GTTAAC GT[CT]^[GA]AC 4584 GTCAAC MamI 2827 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3651 GATTCATATC MfeI 363 CAATTG C^AATTG 4662 CAATTG MflI 168 AGATCC [GA]^GATC[CT] 742 AGATCT MspA1I 968 CAGCTG C[AC]G^C[GT]G 5133 CAGCTG MunI 363 CAATTG C^AATTG 4662 CAATTG MvnI 1809 CGCG CG^CG 4748 CGCG NcoI 907 CCATGG C^CATGG 3786 CCATGG NdeI 2908 CATATG CA^TATG 2955 CATATG PflMI 329 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1520 CCAGGATTTGG PvuII 968 CAGCTG CAG^CTG 5133 CAGCTG Van91I 329 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1520 CCAGGATTTGG XhoII 168 AGATCC [GA]^GATC[CT] 742 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccI AciI AcsI AfaI AflII AflIII AluI AlwI ApoI AseI AsnI AvaII BbvI BfaI BfrI BpmI BsaJI BsiYI BslI BsmAI BsmFI BsmI Bsp1407I BspLU11I BspMI BsrDI BsrGI BsrI BssKI Bst1107I BstF5I BstNI BstZ17I Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HgaI HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PleI PshBI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI