##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccII AflII AflIII AgeI
AhdI Aor51HI AscI AseI AsnI
AspEI AspI AviII AvrII BcgI
BfrI BglI BlnI BlpI Bpu1102I
BsiEI BsiWI BsmBI BspDI BspLU11I
BsrBI BssHII BssSI BstUI CelII
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I FauI FseI
FspI KspI MluI MspA1I MvnI
NheI NotI NruI PacI PflFI
PinAI PmeI PshBI PvuI PvuII
RsrII SacI SacII SalI SbfI
SexAI SfiI SmaI SnaBI Sse8387I
SwaI Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AclI 4437 AACGTT AA^CGTT
AcyI 636 GGCGCC G[GA]^CG[CT]C
Alw44I 3620 GTGCAC G^TGCAC
ApaLI 3620 GTGCAC G^TGCAC
BamHI 2198 GGATCC G^GATCC
BbeI 636 GGCGCC GGCGC^C
BbvCI 648 GCTGAGG CCTCAGC(-5/-2)
BcnI 3101 CCCGG CC^[GC]GG
BsaHI 636 GGCGCC G[GA]^CG[CT]C
BsgI 3323 GTGCAG GTGCAG(16/14)
Bsp1407I 863 TGTACA T^GTACA
BsrDI 5620 CATTGC GCAATG(2/0)
BsrGI 863 TGTACA T^GTACA
Bst1107I 2480 GTATAC GTA^TAC
BstBI 5343 TTCGAA TT^CGAA
BstEII 1367 GGTTACC G^GT[ACGT]ACC
BstPI 1367 GGTTACC G^GT[ACGT]ACC
BstXI 120 CCAGATGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2480 GTATAC GTA^TAC
EcoO65I 1367 GGTTACC G^GT[ACGT]ACC
EcoRV 909 GATATC GAT^ATC
HgaI 4003 GCGTC GACGC(5/10)
Hin1I 636 GGCGCC G[GA]^CG[CT]C
KasI 636 GGCGCC G^GCGCC
MfeI 5014 CAATTG C^AATTG
MunI 5014 CAATTG C^AATTG
NaeI 3056 GCCGGC GCC^GGC
NarI 636 GGCGCC GG^CGCC
NciI 3101 CCCGG CC^[GC]GG
NgoMIV 3056 GCCGGC G^CCGGC
NspV 5343 TTCGAA TT^CGAA
PaeR7I 1357 CTCGAG C^TCGAG
PflMI 3775 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3429 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4437 AACGTT AA^CGTT
SapI 3286 GCTCTTC GCTCTTC(1/4)
ScaI 2103 AGTACT AGT^ACT
SfoI 636 GGCGCC GGC^GCC
SfuI 5343 TTCGAA TT^CGAA
SgrAI 2798 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 3294 ACTAGT A^CTAGT
SphI 5402 GCATGC GCATG^C
SspBI 863 TGTACA T^GTACA
StuI 3523 AGGCCT AGG^CCT
Van91I 3775 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3845 CCATTTCAACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1357 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 2515 TCCGGA T^CCGGA
3530 TCCGGA
ApaI 3098 GGGCCC GGGCC^C
3353 GGGCCC
Asp700I 20 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3803 GAACCAGTTC
AspHI 3620 GTGCAC G[AT]GC[AT]^C
3936 GAGCAC
AvaI 1357 CTCGAG C^[CT]CG[GA]G
3592 CCCGAG
BalI 939 TGGCCA TGG^CCA
3142 TGGCCA
BanII 3098 GGGCCC G[GA]GC[CT]^C
3353 GGGCCC
BbrPI 3222 CACGTG CAC^GTG
5323 CACGTG
BbsI 1824 GAAGAC GAAGAC(2/6)
3789 GAAGAC
BclI 712 TGATCA T^GATCA
805 TGATCA
BpuAI 1824 GAAGAC GAAGAC(2/6)
3789 GAAGAC
BsaAI 3222 CACGTG [CT]AC^GT[GA]
5323 CACGTG
BsaBI 995 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2786 GATTGCCATC
BseAI 2515 TCCGGA T^CCGGA
3530 TCCGGA
BsiHKAI 3620 GTGCAC G[AT]GC[AT]^C
3936 GAGCAC
BsmI 1127 GAATGC GAATGC(1/-1)
1868 GAATGC
BsoBI 1357 CTCGAG C^[CT]CG[GA]G
3592 CCCGAG
BspEI 2515 TCCGGA T^CCGGA
3530 TCCGGA
BspMI 1052 GCAGGT ACCTGC(4/8)
5252 GCAGGT
BstAPI 266 GCAATTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3909 GCAGGAGGTGC
DraIII 1314 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
3219 CACCACGTG
EcoNI 379 CCTGTTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
843 CCTGACACAGG
FbaI 712 TGATCA T^GATCA
805 TGATCA
HpaI 2658 GTTAAC GTT^AAC
4431 GTTAAC
MamI 995 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2786 GATTGCCATC
MluNI 939 TGGCCA TGG^CCA
3142 TGGCCA
MroI 2515 TCCGGA T^CCGGA
3530 TCCGGA
MscI 939 TGGCCA TGG^CCA
3142 TGGCCA
NcoI 2806 CCATGG C^CATGG
4082 CCATGG
NspI 3986 ACATGC [GA]CATG^[CT]
5402 GCATGC
PmaCI 3222 CACGTG CAC^GTG
5323 CACGTG
PmlI 3222 CACGTG CAC^GTG
5323 CACGTG
XmnI 20 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3803 GAACCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
Acc65I AccI AciI AcsI AfaI
AluI AlwI AlwNI AocI ApoI
Asp718I AvaII BanI BbvI BciVI
BfaI BglII BmyI BpmI BsaI
BsaJI BsaWI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NdeI NdeII NlaIII NlaIV
NsiI PleI PpuMI PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII